中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2008年
2期
136-140
,共5页
谭兴容%杨刚毅%李伶%王毅%李钶%齐晓亚%朱伟%孙迅%唐毅%刘华%Guenther Boden
譚興容%楊剛毅%李伶%王毅%李鈳%齊曉亞%硃偉%孫迅%唐毅%劉華%Guenther Boden
담흥용%양강의%리령%왕의%리아%제효아%주위%손신%당의%류화%Guenther Boden
Exenatide%胰岛素抵抗%糖脂代谢%脂联素
Exenatide%胰島素牴抗%糖脂代謝%脂聯素
Exenatide%이도소저항%당지대사%지련소
Exenatide%Insulin resistance%Glucose-lipid metabolism%Adiponectin
目的 探讨Exenatide对高脂诱导胰岛素抵抗大鼠胰岛β细胞功能、胰岛素敏感性及糖脂代谢的影响. 方法 高脂诱导胰岛素抵抗大鼠给予Exenatide 6周后,采用静脉葡萄糖耐量(IVGTT)和胰岛素耐量(ITT)试验以及扩展胰岛素钳夹技术测定胰岛素敏感性和糖脂代谢,并观察血浆脂联素水平的变化.结果 高脂大鼠(HF)经Exenatide处理后,Lee′s指数、空腹血浆游离脂肪酸(FFA)、甘油三酯、胆固醇明显降低(均P＜0.01);IVGTT和ITT明显改善,胰岛素分泌水平增高,高剂量组(HFH)较低剂量组(HFL)上述指标改善更为明显.同时,HFH组血浆脂联素水平也明显升高(P＜0.01).在钳夹稳态时,HF组与对照组(NC)相比,血浆FFA、胰岛素水平均明显升高(均P＜0.01),葡萄糖输注率(GIR)、葡萄糖清除率(GRd)明显降低(均P＜0.01),且胰岛素对肝糖输出(HGP)的抑制作用明显障碍(仅抑制26%).经Exenatide(2 μg/kg)处理以后,血浆FFA、胰岛素水平则明显降低(均P＜0.01),GRd、GIR明显升高(均P＜0.01),胰岛素对HGP的抑制作用明显增强(抑制72%).结论 对高脂喂养大鼠用Exenatide预处理可能通过促进β细胞胰岛素分泌和上调血浆脂联素水平,改善糖脂代谢而使机体胰岛素敏感性增加.
目的 探討Exenatide對高脂誘導胰島素牴抗大鼠胰島β細胞功能、胰島素敏感性及糖脂代謝的影響. 方法 高脂誘導胰島素牴抗大鼠給予Exenatide 6週後,採用靜脈葡萄糖耐量(IVGTT)和胰島素耐量(ITT)試驗以及擴展胰島素鉗夾技術測定胰島素敏感性和糖脂代謝,併觀察血漿脂聯素水平的變化.結果 高脂大鼠(HF)經Exenatide處理後,Lee′s指數、空腹血漿遊離脂肪痠(FFA)、甘油三酯、膽固醇明顯降低(均P＜0.01);IVGTT和ITT明顯改善,胰島素分泌水平增高,高劑量組(HFH)較低劑量組(HFL)上述指標改善更為明顯.同時,HFH組血漿脂聯素水平也明顯升高(P＜0.01).在鉗夾穩態時,HF組與對照組(NC)相比,血漿FFA、胰島素水平均明顯升高(均P＜0.01),葡萄糖輸註率(GIR)、葡萄糖清除率(GRd)明顯降低(均P＜0.01),且胰島素對肝糖輸齣(HGP)的抑製作用明顯障礙(僅抑製26%).經Exenatide(2 μg/kg)處理以後,血漿FFA、胰島素水平則明顯降低(均P＜0.01),GRd、GIR明顯升高(均P＜0.01),胰島素對HGP的抑製作用明顯增彊(抑製72%).結論 對高脂餵養大鼠用Exenatide預處理可能通過促進β細胞胰島素分泌和上調血漿脂聯素水平,改善糖脂代謝而使機體胰島素敏感性增加.
목적 탐토Exenatide대고지유도이도소저항대서이도β세포공능、이도소민감성급당지대사적영향. 방법 고지유도이도소저항대서급여Exenatide 6주후,채용정맥포도당내량(IVGTT)화이도소내량(ITT)시험이급확전이도소겸협기술측정이도소민감성화당지대사,병관찰혈장지련소수평적변화.결과 고지대서(HF)경Exenatide처리후,Lee′s지수、공복혈장유리지방산(FFA)、감유삼지、담고순명현강저(균P＜0.01);IVGTT화ITT명현개선,이도소분비수평증고,고제량조(HFH)교저제량조(HFL)상술지표개선경위명현.동시,HFH조혈장지련소수평야명현승고(P＜0.01).재겸협은태시,HF조여대조조(NC)상비,혈장FFA、이도소수평균명현승고(균P＜0.01),포도당수주솔(GIR)、포도당청제솔(GRd)명현강저(균P＜0.01),차이도소대간당수출(HGP)적억제작용명현장애(부억제26%).경Exenatide(2 μg/kg)처리이후,혈장FFA、이도소수평칙명현강저(균P＜0.01),GRd、GIR명현승고(균P＜0.01),이도소대HGP적억제작용명현증강(억제72%).결론 대고지위양대서용Exenatide예처리가능통과촉진β세포이도소분비화상조혈장지련소수평,개선당지대사이사궤체이도소민감성증가.
Objective To investgate the effects of exenatide on islet β-cell function, insulin sensitivity and glucose-lipid metabolism in insulin resistant rats induced by high-fat-chow. Methods High fat-fed rats were treated with exenatide for 6 weeks. The insulin sensitivity, islet β-cell function and glucose lipid metabolism in awake rats were evaluated by intravenous glucose tolerance test (IVGTT), insulin tolerance test (ITT) and hyperinsulinemic-euglycemic clamp technique combined with 3-[3H] glucose as a tracer. In addition, plasma adiponectin level was measured by ELISA. Results Lee′s index and levels of plasma free fatty acids (FFA), triglyceride and total cholesterol were significantly reduced in high fat-fed rats after exenatide treatment for 6 weeks (all P＜0.01). In these rats exenatide also improved IVGTT and ITT, and increased the level of insulin secretion, especially when a high dose was given. In addition, plasma adiponectin level was also significantly increased in the group with high dose exenatide (HFH, P＜0.01). During the clamp steady-state, there were significant increases in plasma FFA and insulin and significant decreases in glucose infusion rate (GIR), glucose disposal rate (GRd) in high-fat group (HF) compared to control group (NC, all P＜0.01). The suppressive effect of insulin on hepatic glucose production (HGP) was significantly blunted (only 26%) in HF group. In HFH group, plasma insulin and FFA levels were significantly decreased (both P＜0.01), GIR and GRd were significantly increased (both all P＜0.01), and HGP was suppressed by 72%. Conclusion It is possible that exenatide pretreatment ameliorates high-fat induced insulin resistance by promoting β-cell insulin secretion, elevating adiponectin level, and improving glucose-lipid metabolism.
