中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2010年
8期
486-489
,共4页
胡肖伟%张小平%李壁如%张建%王莹
鬍肖偉%張小平%李壁如%張建%王瑩
호초위%장소평%리벽여%장건%왕형
角化细胞生长因子%急性肺损伤%脂多糖%Ⅱ型肺泡上皮细胞
角化細胞生長因子%急性肺損傷%脂多糖%Ⅱ型肺泡上皮細胞
각화세포생장인자%급성폐손상%지다당%Ⅱ형폐포상피세포
Keratinocyte growth factor%Acute lung injury%Lipopolysaccharide%Type Ⅱ alveolar epithelial cell
目的 初步研究角化细胞生长因子(KGF)对脂多糖(LPS)诱导大鼠急性肺损伤(ALI)的保护作用及可能的机制.方法 将36只SD大鼠按随机数字表法分为3组,每组12只.模型组尾静脉注射LPS 5 mg/kg建立ALI动物模型.对照组和KGF组注射等量生理盐水.KGF组在注射LPS后气道给予KGF 5 mg/kg.8 h后处死各组大鼠观察肺组织病理改变,并测量肺血管通透性、肺上皮细胞通透性、肺湿/干重(W/D)比值以及Ⅱ型肺泡上皮细胞(ATⅡ)增殖、修复功能改变.结果 光镜下观察显示KGF可有效减轻LPS所致ALI的肺组织病理改变,表现为肺血管充血、水肿减轻,几乎无炎性细胞浸润.与模型组比较,KGF组肺血管通透性[(0.026±0.049)%比(0.087±0.027)%]和肺泡上皮通透性[(0.692±0.017)%比(0.931±0.029)%]及W/D比值(4.778±0.243比6.869±0.153)均明显降低(P<0.05或P<0.01),ATⅡ细胞增殖及修复功能则明显提高[ATⅡ数量(个):6.083±1.781比4.666±1.923,损伤面积(mm2):2.946±0.453比6.181±0.975,P<0.05和P<0.01].结论 KGF可减轻LPS所致ALI,其机制可能是通过增强ATⅡ细胞的增殖及修复能力从而起到有效的保护作用.
目的 初步研究角化細胞生長因子(KGF)對脂多糖(LPS)誘導大鼠急性肺損傷(ALI)的保護作用及可能的機製.方法 將36隻SD大鼠按隨機數字錶法分為3組,每組12隻.模型組尾靜脈註射LPS 5 mg/kg建立ALI動物模型.對照組和KGF組註射等量生理鹽水.KGF組在註射LPS後氣道給予KGF 5 mg/kg.8 h後處死各組大鼠觀察肺組織病理改變,併測量肺血管通透性、肺上皮細胞通透性、肺濕/榦重(W/D)比值以及Ⅱ型肺泡上皮細胞(ATⅡ)增殖、脩複功能改變.結果 光鏡下觀察顯示KGF可有效減輕LPS所緻ALI的肺組織病理改變,錶現為肺血管充血、水腫減輕,幾乎無炎性細胞浸潤.與模型組比較,KGF組肺血管通透性[(0.026±0.049)%比(0.087±0.027)%]和肺泡上皮通透性[(0.692±0.017)%比(0.931±0.029)%]及W/D比值(4.778±0.243比6.869±0.153)均明顯降低(P<0.05或P<0.01),ATⅡ細胞增殖及脩複功能則明顯提高[ATⅡ數量(箇):6.083±1.781比4.666±1.923,損傷麵積(mm2):2.946±0.453比6.181±0.975,P<0.05和P<0.01].結論 KGF可減輕LPS所緻ALI,其機製可能是通過增彊ATⅡ細胞的增殖及脩複能力從而起到有效的保護作用.
목적 초보연구각화세포생장인자(KGF)대지다당(LPS)유도대서급성폐손상(ALI)적보호작용급가능적궤제.방법 장36지SD대서안수궤수자표법분위3조,매조12지.모형조미정맥주사LPS 5 mg/kg건립ALI동물모형.대조조화KGF조주사등량생리염수.KGF조재주사LPS후기도급여KGF 5 mg/kg.8 h후처사각조대서관찰폐조직병리개변,병측량폐혈관통투성、폐상피세포통투성、폐습/간중(W/D)비치이급Ⅱ형폐포상피세포(ATⅡ)증식、수복공능개변.결과 광경하관찰현시KGF가유효감경LPS소치ALI적폐조직병리개변,표현위폐혈관충혈、수종감경,궤호무염성세포침윤.여모형조비교,KGF조폐혈관통투성[(0.026±0.049)%비(0.087±0.027)%]화폐포상피통투성[(0.692±0.017)%비(0.931±0.029)%]급W/D비치(4.778±0.243비6.869±0.153)균명현강저(P<0.05혹P<0.01),ATⅡ세포증식급수복공능칙명현제고[ATⅡ수량(개):6.083±1.781비4.666±1.923,손상면적(mm2):2.946±0.453비6.181±0.975,P<0.05화P<0.01].결론 KGF가감경LPS소치ALI,기궤제가능시통과증강ATⅡ세포적증식급수복능력종이기도유효적보호작용.
Objective To investigate the protective effect of keratinocyte growth factor (KGF) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and its potential mechanism in rats. Methods Thirty-six Sprague-Dawley (SD) rats were randomly divided into three groups, each group with 12 rats. LPS (5 mg/kg) was injected intravenously to induce ALI in model group, and same amount of normal saline was injected in control group and KGF group. The rats in KGF group were treated with KGF (5 mg/kg) intratracheally after injection of LPS. The rats were sacrificed after 8 hours, histologic assessments, wet/dry weight (W/D) ratio, pulmonary vascular permeability, lung epithelial cell permeability, the proliferation and repair capacity of type Ⅱ alveolar epithelial cells (ATⅡ) were analyzed. Results Under optical microscope, it was found that KGF could reduce injury to lung tissue induced by LPS. Compared with the model group, KGF could decrease pulmonary vascular permeability [(0.026±0.049)% vs. (0.087±0.027)%], lung epithelial cell permeability [(0.692±0.017)% vs. (0.931±0.029)%] and W/D ratio (4.778±0.243 vs. 6.869±0.153, P<0.05 or P<0.01), enhance the proliferation and repair capacity of ATⅡ cells (ATⅡ cells: 6.083±1.781 vs. 4.666±1.923, injury area (mm2): 2.946±0.453 vs. 6.181±0.975, P<0.05 and P<0.01). Conclusion KGF could reduce the injury to the lung in LPS-induced ALI, and it plays a protective role through enhancing the proliferation and repair capacity of ATⅡ cells.
