中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2010年
3期
147-150
,共4页
石燕%王胜军%陈建国%薛渊%何志强%周成林%郑东%杨恒%李雅贞%仝佳%苏兆亮%邵启祥%许化溪
石燕%王勝軍%陳建國%薛淵%何誌彊%週成林%鄭東%楊恆%李雅貞%仝佳%囌兆亮%邵啟祥%許化溪
석연%왕성군%진건국%설연%하지강%주성림%정동%양항%리아정%동가%소조량%소계상%허화계
关节炎,类风湿%高迁移率族蛋白类%白细胞介素17
關節炎,類風濕%高遷移率族蛋白類%白細胞介素17
관절염,류풍습%고천이솔족단백류%백세포개소17
Arthritis,rheumatoid%High mobility group proteins%Interleukin-17
目的 检测类风湿关节炎(RA)患者外周血高迁移率族蛋白1(HMGB1)及Th17细胞特异性转录因子、相关细胞因子的表达水平,同时分析其与C反应蛋白(CRP)、红细胞沉降率(ESR)和类风湿因子(RF)的关系,初步探讨RA患者HMGB1的表达水平及其与Th17的关系.方法 收集80例RA患者外周血标本,其中静止期32例、活动期48例,健康志愿者50名.采用实时荧光定量聚合酶链反应(QRT-PCR)检测外周血单个核细胞(PBMC)中HMGB1、RORγt和白细胞介素(IL)-17的mRNA水平;酶联免疫吸附试验(ELISA)检测血浆HMGB1、IL-17、IL-23的蛋白浓度,采用单因素方差分析和Spearman相关分析进行统计学处理.结果 RA患者PBMC中HMGB1、RORyt和IL-17 mRNA的表达水平均高于健康对照组(P<0.05).且活动期[HMGB1(0.424±0.262)pg/ml,RORγt(0.34±0.25)pg/ml,IL-17(1.42±0.38)pg/ml]明显高于静止期(P<0.01).o血浆HMGB1、IL-23和IL-17蛋白水平也显示类似的结果,RA组明显高于健康对照组(P<0.05),且与CRP、ESR、RF呈正相关(P<0.05).结论 检测RA患者外周血HMGB1和Th17细胞特异性转录因子及相关细胞因子水平,有助于探讨HMGB1和IL-17在RA发病过程中的病理生理作用,也为寻找RA的治疗靶点提供了新的思路.
目的 檢測類風濕關節炎(RA)患者外週血高遷移率族蛋白1(HMGB1)及Th17細胞特異性轉錄因子、相關細胞因子的錶達水平,同時分析其與C反應蛋白(CRP)、紅細胞沉降率(ESR)和類風濕因子(RF)的關繫,初步探討RA患者HMGB1的錶達水平及其與Th17的關繫.方法 收集80例RA患者外週血標本,其中靜止期32例、活動期48例,健康誌願者50名.採用實時熒光定量聚閤酶鏈反應(QRT-PCR)檢測外週血單箇覈細胞(PBMC)中HMGB1、RORγt和白細胞介素(IL)-17的mRNA水平;酶聯免疫吸附試驗(ELISA)檢測血漿HMGB1、IL-17、IL-23的蛋白濃度,採用單因素方差分析和Spearman相關分析進行統計學處理.結果 RA患者PBMC中HMGB1、RORyt和IL-17 mRNA的錶達水平均高于健康對照組(P<0.05).且活動期[HMGB1(0.424±0.262)pg/ml,RORγt(0.34±0.25)pg/ml,IL-17(1.42±0.38)pg/ml]明顯高于靜止期(P<0.01).o血漿HMGB1、IL-23和IL-17蛋白水平也顯示類似的結果,RA組明顯高于健康對照組(P<0.05),且與CRP、ESR、RF呈正相關(P<0.05).結論 檢測RA患者外週血HMGB1和Th17細胞特異性轉錄因子及相關細胞因子水平,有助于探討HMGB1和IL-17在RA髮病過程中的病理生理作用,也為尋找RA的治療靶點提供瞭新的思路.
목적 검측류풍습관절염(RA)환자외주혈고천이솔족단백1(HMGB1)급Th17세포특이성전록인자、상관세포인자적표체수평,동시분석기여C반응단백(CRP)、홍세포침강솔(ESR)화류풍습인자(RF)적관계,초보탐토RA환자HMGB1적표체수평급기여Th17적관계.방법 수집80례RA환자외주혈표본,기중정지기32례、활동기48례,건강지원자50명.채용실시형광정량취합매련반응(QRT-PCR)검측외주혈단개핵세포(PBMC)중HMGB1、RORγt화백세포개소(IL)-17적mRNA수평;매련면역흡부시험(ELISA)검측혈장HMGB1、IL-17、IL-23적단백농도,채용단인소방차분석화Spearman상관분석진행통계학처리.결과 RA환자PBMC중HMGB1、RORyt화IL-17 mRNA적표체수평균고우건강대조조(P<0.05).차활동기[HMGB1(0.424±0.262)pg/ml,RORγt(0.34±0.25)pg/ml,IL-17(1.42±0.38)pg/ml]명현고우정지기(P<0.01).o혈장HMGB1、IL-23화IL-17단백수평야현시유사적결과,RA조명현고우건강대조조(P<0.05),차여CRP、ESR、RF정정상관(P<0.05).결론 검측RA환자외주혈HMGB1화Th17세포특이성전록인자급상관세포인자수평,유조우탐토HMGB1화IL-17재RA발병과정중적병리생리작용,야위심조RA적치료파점제공료신적사로.
Objective To detect the expression levels of high mobility group box chromosomal protein 1 (HMGB1) and Th17 cells transcription factors, related cytokines in peripheral blood of rheumatoid arthritis (RA) patients and analyze the relations between HMGB1 and CRP, ESR, RF in RA patients. The other aim of this study is to identify the expression level of HMGBI and the relationship between HMGB1 and Th17 in RA patients. Methods The mRNA levels of HMGB1, RORyt, interleukin (IL)-17 in the peripheral blood mononuclear cells (PBMC) were determined by quantitative real-time PCR (QRT-PCR) from 80 patients with rheumatoid arthritis,including 32 RA patients in stable phase and 48 patients in active phase, and 50 healthy volunteers. The concentration of HMGB1, IL-23, IL-17 in plasma were detected by enzyme linked immunosorbent assay (ELISA), one-way ANOVA and Spearman's correleation were adopted for statistical analysis.Results The mRNAs of HMGBI, RORyt and IL-17 in RA patients were higher than that in healthy control group (P<0.05), especially in active RA patients [ HMGB 1 (0.424±0.262) pg/ml, RORγt (0.34±0.25) pg/ml,IL-17 (1.42±0.38) pg/ml,P<0.01 ] when compared with patients with stable disease. The concentration of HMGB1, IL-23 and IL-17 in the plasma of RA patients was higher than that of the healthy control group (P< 0.05), and was positively correlated with the expression levels of HMGB1, Th 17-associated factors and the level of CRP, ESR, RF in RA patients' plasma(P<0.05). Conclusion The HMGB1 and Thl7 cells levels are higher in active RA patients than those in patients with stable disease, arid there is significant positive correlation between them. Detection of peripheral HMGB1 and Thl7 cell-specific transcription factors or related cytokines can help to understand the development and progress of rheumatoid arthritis and provide clues for new treatment targets for RA.
