CAJ | 학술논문

目的探讨多巴胺递质代谢相关蛋白儿茶酚氧位甲基转移酶(COMT)Val108/158Met、多巴胺转运体(DAT)40bp可变数目串联重复多态(VNTR)两位点基因多态性与卒中后抑郁(PSD)的关系.方法选择南方医科大学珠江医院神经内科自2010年1月至2010年6月收治的PSD患者68例作为研究组,同期脑卒中后无抑郁患者91例作为对照组,利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术分析患者COMT Val108/158Met、DAT 40 bp VNTR位点的基因多态性.结果 COMT基因扩增产物分野生型(G/G)、突变纯合型(A/A)、杂合型(A/G)3种基因型,DAT基因扩增产物分7/7、9/7、10/7、10/9、10/10、11/10、11/11倍重复7种基因型;2组患者COMT等位基因、基因型分布比较差异均有统计学意义(x2=5.703,P=0.017;x2=6.489,P=0.039).研究组男性患者与对照组男性患者之间比较,COMT等位基因、基因型的分布差异均无统计学意义(P＞0.05);研究组女性患者与对照组女性患者之间比较,COMT等位基因、基因型的分布差异均有统计学意义(x2=4.610,P=0.032;x2=6.547,P=0.024);研究组与对照组患者DAT等位基因和基因型的总体分布、杂合度比较差异均无统计学意义(P＞0.05).结论 COMT Val108/158Met基因多态性与PSD发生可能有相关性,而DAT40 bp VNTR基因多态性与PSD发生无明显相关性.
목적 탐토다파알체질대사상관단백인다분양위갑기전이매(COMT)Val108/158Met、다파알전운체(DAT)40bp가변수목천련중복다태(VNTR)량위점기인다태성여졸중후억욱(PSD)적관계.방법 선택남방의과대학주강의원신경내과자2010년1월지2010년6월수치적PSD환자68례작위연구조,동기뇌졸중후무억욱환자91례작위대조조,이용취합매련식반응-한제성편단장도다태성(PCR-RFLP)기술분석환자COMT Val108/158Met、DAT 40 bp VNTR위점적기인다태성.결과 COMT기인확증산물분야생형(G/G)、돌변순합형(A/A)、잡합형(A/G)3충기인형,DAT기인확증산물분7/7、9/7、10/7、10/9、10/10、11/10、11/11배중복7충기인형;2조환자COMT등위기인、기인형분포비교차이균유통계학의의(x2=5.703,P=0.017;x2=6.489,P=0.039).연구조남성환자여대조조남성환자지간비교,COMT등위기인、기인형적분포차이균무통계학의의(P＞0.05);연구조녀성환자여대조조녀성환자지간비교,COMT등위기인、기인형적분포차이균유통계학의의(x2=4.610,P=0.032;x2=6.547,P=0.024);연구조여대조조환자DAT등위기인화기인형적총체분포、잡합도비교차이균무통계학의의(P＞0.05).결론 COMT Val108/158Met기인다태성여PSD발생가능유상관성,이DAT40 bp VNTR기인다태성여PSD발생무명현상관성.
Objective To investigate the association of post-stroke depression (PSD) with gene polymorphisms of catechol-O-methyl transferase (COMT) Val1 08/158Met and dopamine transporter 40bp variable number of tandem repeats (VNTR) in dopamine metabolism system. Methods Sixty-eight patients with PSD and 91 patients only suffered from stroke, admitted to our hospital from January 2010to June 2010, were chosen; the gene polymorphisms ofCOMT Val108/158Met and DAT 40 bp VNTR were analyzed by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The genotypes of COMT gene amplifications were wild type (G/G),homozygous mutant type (A/A) and heterozygous type (A/G); 7 repeated genotypes (7/7, 9/7, 10/7, 10/9,10/10, 11/10 and 11/11) were noted in the DA T gene amplifications; frequencies of COMT alleles and genotypes were significantly different between the 2 groups (x2=5.703, P=0.017;x2=6.489, P=0.039). The frequencies of COMT alleles and genotypes were significantly different between the 2 female groups (x2=4.610, P=0.032;x2=6.547, P=0.024), but no significant differences were found between the 2 male groups (P＞0.05). The frequencies and heterozygosity of DAT alleles and genotypes showed no obvious differences between the 2 groups (P＞0.05). Conclusion The gene polymorphism of COMT Val108/158Met may be associated with PSD, while that of DAT 40bp VNTR is not.