CAJ | 학술논문

目的研究成人乙肝疫苗免疫失败与HLA-DR、T细胞亚群相关性,探讨与成人乙肝疫苗免疫失败相关因素.方法随机选取接种10μg重组酵母乙型肝炎疫苗免疫失败者及成功者各20名,检测T细胞亚群及HLA-DR、HLA-B27、HLA DRB1 * 07、DRB1 * 04、DRB1 * 1001、DQB1 * 0401等DR区基因表型水平.结果免疫失败组CD4-/CD8-含量低于对照组,免疫失败组HLA-DR及HLA-B27平均水平高于对照组,两组间HLA DRB1 * 07基因频率差异有统计学意义(P<0.05),而两组间CD3、CD4、CD8、CD5、CD7、CD4+/CD8+、CD4-/CD8+、CD4+/CD8-含量及HLA DRB1 * 04、DRB1 * 1001、DQB1*0401水平差异均无统计学意义.结论成人乙肝免疫失败可能与HLA-DR、HLA-B27、HLA DRB1 *07、CD4-/CD8-等相关.
목적 연구성인을간역묘면역실패여HLA-DR、T세포아군상관성,탐토여성인을간역묘면역실패상관인소.방법 수궤선취접충10μg중조효모을형간염역묘면역실패자급성공자각20명,검측T세포아군급HLA-DR、HLA-B27、HLA DRB1 * 07、DRB1 * 04、DRB1 * 1001、DQB1 * 0401등DR구기인표형수평.결과 면역실패조CD4-/CD8-함량저우대조조,면역실패조HLA-DR급HLA-B27평균수평고우대조조,량조간HLA DRB1 * 07기인빈솔차이유통계학의의(P<0.05),이량조간CD3、CD4、CD8、CD5、CD7、CD4+/CD8+、CD4-/CD8+、CD4+/CD8-함량급HLA DRB1 * 04、DRB1 * 1001、DQB1*0401수평차이균무통계학의의.결론 성인을간면역실패가능여HLA-DR、HLA-B27、HLA DRB1 *07、CD4-/CD8-등상관.
Objective To study and explore the relativity of adults HBV vicinal failure and HLA-DR,T cell subset, trace viruses infection. To accumulate date for formulating preventing adult HBV infection prophylactic-therapeutic measures. Methods Select 20 adults randomly who had vaccinated with 10 μg YDV and produced anti-HBS successfully, and another 20 hadn't produced anti-HBs to form two groups-defeated group and contral group. Blood samples from two groups were taken for detecting the level of DR range gene phenotype: T cell subset, white blood cell HLA-DR, HLA-B27, HLA DRB1 * 07, DRB1 * 04, DRB1 * 1001, DQB1 * 0401 and so on. Results The level of CD4-/CD8- is lower in the infection group than in healthy group. But the average level of HLA-DR and HLA-B27 is higher in the infection group. The differences of HLA DRB1 * 07 gene frequency between two groups were significant ( P < 0.05), but the levels of CD3, CD4, CDS, CD7, CD4/CD8 and HLA DRB1 * 04, DRB1 * 1001, DQB1 * 0401 were not significant. Conclusion The failure of HBV vaccination on adults may have relation to HLA-DR, HLA-B27, HLA DRB1 * 07, CD4-/CD8- , etc.