CAJ | 학술논문

目的检测人原发性肝细胞肝癌组织中组织蛋白酶D(CD)、组织蛋白酶H(CH)和组织蛋白酶L(CL)表达,探讨其作用与机制.方法应用免疫组织化学法测定82例肝组织标本CD、CH和CL蛋白的表达,包括对照组17例,肝细胞肝癌(HCC)组37例和肝细胞肝癌癌旁组织(PTT)组28例,采用吸光度值分析其相对含量.数据采用SPSS 11.5软件统计分析,组间比较采用单因素方差分析.结果 HCC组CD(1.21±0.33)、CH(0.89±0.22)和CL蛋白(1.16±0.25)的平均吸光度值均明显高于对照组(分别为0.19±0.07,0.24±0.12和0.28±0.14)和PTT组(分别为0.27±0.13,0.31±0.14和0.36±0.15),差异有统计学意义(均P=0.0001),PTT组与对照组间3种蛋白表达均无明显差异(均P＞0.05).3种蛋白免疫组织化学表达形态显示,HCC组呈大量密集成弥漫性的点、片状深棕色满布整个细胞质,对照组和PTT组均仅见细胞质中有散在点状浅棕色分布.结论人肝癌组织CD、CH和CL蛋白高表达,可能是反映原发性肝癌发生发展的重要标记物之一.
목적 검측인원발성간세포간암조직중조직단백매D(CD)、조직단백매H(CH)화조직단백매L(CL)표체,탐토기작용여궤제.방법 응용면역조직화학법측정82례간조직표본CD、CH화CL단백적표체,포괄대조조17례,간세포간암(HCC)조37례화간세포간암암방조직(PTT)조28례,채용흡광도치분석기상대함량.수거채용SPSS 11.5연건통계분석,조간비교채용단인소방차분석.결과 HCC조CD(1.21±0.33)、CH(0.89±0.22)화CL단백(1.16±0.25)적평균흡광도치균명현고우대조조(분별위0.19±0.07,0.24±0.12화0.28±0.14)화PTT조(분별위0.27±0.13,0.31±0.14화0.36±0.15),차이유통계학의의(균P=0.0001),PTT조여대조조간3충단백표체균무명현차이(균P＞0.05).3충단백면역조직화학표체형태현시,HCC조정대량밀집성미만성적점、편상심종색만포정개세포질,대조조화PTT조균부견세포질중유산재점상천종색분포.결론 인간암조직CD、CH화CL단백고표체,가능시반영원발성간암발생발전적중요표기물지일.
Objective To determine the expression of cathepsin D (CD), cathepsin H (CH) and cathepsin L (CL) in human primary hepatocellular carcinoma (HCC), and to investigate their mechanisms. Methods The protein expression of CD, CH and CL in hepatic specimens consisted of control (n = 17), HCC (n = 37) and paracancerous (n = 28) tissues were detected by immunohistochemistry. The relative values of CD, CH and CL protein expression were examined with absorbent density analysis. Data were analyzed using SPSS11. 5 software. The univariate analysis was used to compare the difference among groups. Results The mean absorbance of CD, CH and CL proteins in HCC tissues (1.21± 0.33, 0. 89 ± 0.22 and 1.16± 0. 25, respectively) were significantly higher in comparison with those in control tissues (0. 19 ± 0. 07, 0. 24 ± 0. 12 and 0. 28 ± 0. 14,respectively) and in paracancerous tissues (0.27±0.13,0. 31± 0.14 and 0. 36±0.15)(all P values =0.0001). Whereas there was no difference between control and paracancerous tissues with respect to CD, CH and CL proteins (P ＞0. 05). Three proteins immunohistochemically appeared as a lot of diffused spots and stripes staining in cytoplasma of HCC tissues,but only a few scatted spots staining was found in control and paracancerous tissues. Conclusion The high expression of CD, CH and CL protein in primary HCC may be important markers for carcinogenesis and malignant progress.