遗传
遺傳
유전
HEREDITAS(BEIJING)
2001年
2期
147-150
,共4页
PCR%cDNAPCR文库%反转录反应
PCR%cDNAPCR文庫%反轉錄反應
PCR%cDNAPCR문고%반전록반응
使用PCR(polymerase chain reaction)技术,调制了mRNA的cDNA PCR文库,实验证明,cDNA PCR文库能使原cDNA的量放大数百倍。同时,使用人体K562培养细胞的总RNA,对cDNA PCR文库法和反转录中的β-Actin的cDNA量进行了比较,cDNA PCR文库法中的β-Actin的cDNA量大大高于反转录中的β-Actin的cDNA量。使用75pg的人体K562培养细胞的总RNA,调制成50μl的cDNA PCR文库,使用1μl的cDNA PCR文库进行PCR反应时,可对文库中的β-Actin的cDNA进行PCR检测。因此,cDNA PCR文库显示了良好的信息放大性能。
使用PCR(polymerase chain reaction)技術,調製瞭mRNA的cDNA PCR文庫,實驗證明,cDNA PCR文庫能使原cDNA的量放大數百倍。同時,使用人體K562培養細胞的總RNA,對cDNA PCR文庫法和反轉錄中的β-Actin的cDNA量進行瞭比較,cDNA PCR文庫法中的β-Actin的cDNA量大大高于反轉錄中的β-Actin的cDNA量。使用75pg的人體K562培養細胞的總RNA,調製成50μl的cDNA PCR文庫,使用1μl的cDNA PCR文庫進行PCR反應時,可對文庫中的β-Actin的cDNA進行PCR檢測。因此,cDNA PCR文庫顯示瞭良好的信息放大性能。
사용PCR(polymerase chain reaction)기술,조제료mRNA적cDNA PCR문고,실험증명,cDNA PCR문고능사원cDNA적량방대수백배。동시,사용인체K562배양세포적총RNA,대cDNA PCR문고법화반전록중적β-Actin적cDNA량진행료비교,cDNA PCR문고법중적β-Actin적cDNA량대대고우반전록중적β-Actin적cDNA량。사용75pg적인체K562배양세포적총RNA,조제성50μl적cDNA PCR문고,사용1μl적cDNA PCR문고진행PCR반응시,가대문고중적β-Actin적cDNA진행PCR검측。인차,cDNA PCR문고현시료량호적신식방대성능。
By the method of PCR (Polymerase Chain Reaction),we have constructed the cDNA PCR library from mRNA.The cDNA PCR library can amplify the original cDNA up to hundreds of times.With the total RNA of human K562 cultured cell,the cDNA of β-Actin has been obtained by the methods of cDNA PCR library and reverse transcription respectively.As contrast,the amount of β-Actin′s cDNA from the cDNA PCR library is much higher than from reverse transcription.75pg total RNA of human K562 Cultured cell is employed to construct 50μl cDNA PCR library,and the cDNA of β-Actin can even be detected by using 1μl of the library as template to perform the PCR.Therefore cDNA PCR library can greatly enlarge the amount of information.