CAJ | 학술논문

目的:探索应用骨髓基质细胞治疗缺血性脑梗死的可行性和骨髓基质细胞在修复中枢神经损伤方面的作用. 方法:从健康人肋骨取原代骨髓基质细胞后扩增.线栓法制作大鼠大脑中动脉缺血模型 (n=56), 2 h后再灌注. 24 h后治疗组 (n=28)尾静脉注射 3× 106骨髓基质细胞,对照组 (n=28)和正常组 (n=16)尾静脉注射 1 mL生理盐水.采用神经损伤评分 (NSS)检测大鼠神经系统功能, ELISA检测脑源性神经生长因子 (BDNF)、神经生长因子 (NGF),流式细胞仪检测细胞凋亡,免疫组化方法检测脑内骨髓基质细胞或骨髓基质细胞来源的细胞. 结果:在模型制作后 7, 14, 21, 28 d,治疗组的 NSS较对照组明显低 (t=9.9～ 2.9,P< 0.05),治疗组的 BDNF和 NGF较对照组明显高 (F=708.9,846.2,P< 0.05),治疗组的凋亡和坏死细胞占细胞总数的 (13.6± 7.8)% ,(11.2± 4.5)%,较对照组 [(22.3± 7.8)% ,(16.8± 5.6)% ]明显减少 (F=125.9, 95.7,P< 0.05).在缺血侧半球可以发现骨髓基质细胞,并且一部分骨髓基质细胞表达了神经细胞表面标志物. 结论:骨髓基质细胞移植后缺血脑组织中生长因子的增加和细胞替代对神经功能的恢复起到了重要作用.
목적:탐색응용골수기질세포치료결혈성뇌경사적가행성화골수기질세포재수복중추신경손상방면적작용. 방법:종건강인륵골취원대골수기질세포후확증.선전법제작대서대뇌중동맥결혈모형 (n=56), 2 h후재관주. 24 h후치료조 (n=28)미정맥주사 3× 106골수기질세포,대조조 (n=28)화정상조 (n=16)미정맥주사 1 mL생리염수.채용신경손상평분 (NSS)검측대서신경계통공능, ELISA검측뇌원성신경생장인자 (BDNF)、신경생장인자 (NGF),류식세포의검측세포조망,면역조화방법검측뇌내골수기질세포혹골수기질세포래원적세포. 결과:재모형제작후 7, 14, 21, 28 d,치료조적 NSS교대조조명현저 (t=9.9～ 2.9,P< 0.05),치료조적 BDNF화 NGF교대조조명현고 (F=708.9,846.2,P< 0.05),치료조적조망화배사세포점세포총수적 (13.6± 7.8)% ,(11.2± 4.5)%,교대조조 [(22.3± 7.8)% ,(16.8± 5.6)% ]명현감소 (F=125.9, 95.7,P< 0.05).재결혈측반구가이발현골수기질세포,병차일부분골수기질세포표체료신경세포표면표지물. 결론:골수기질세포이식후결혈뇌조직중생장인자적증가화세포체대대신경공능적회복기도료중요작용.
AIM:To observe the effect of intravenously administration of human bone marrow stromal cells (hMSCs) on neurological functional defects after stroke in rats. METHODS:hMSCs were obtained from healthy human subjects and culture. Rats were subjected to transient middle cerebral artery occlusion and received intravenously administration of 3× 106 hMSCs 1 day later.Neurological function changes were measured before and at 1, 7, 14, and 28 day after cerebral infarction of the rats.Neurotrophin levels in the cerebral tissues of rats with or without hMSC treatment were analyzed by enzyme linked immunosorbent assay (ELISA).Apoptotic cells in the ischemic hemisphere of the rats were analyzed by flow cytometry,and immunohistochemistry was employed to identify hMSCs and cells derived from hMSCs in the rat brain. RESULTS:Significant recovery of neurological functions was observed in rats treated with hMSCs at 7,14,21,and 28 days as compared with ischemic control rats.In the ischemic hemisphere of the rats in the treatment group, brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) significantly increased,and apoptotic cells significantly decreased.With immunohistochemistry,hMSCs were found in the brain tissues of the treated rats,and a few hMSCs expressed proteins phenotypic of brain parenchymal cells. CONCLUSION:Intravenous administration of hMSCs is simple and effective to reduce neurological functional defects after stroke in rats,in which increased growth factors and cell replacement in the ischemic tissues play important roles.