CAJ | 학술논문

目的研究糖尿病大鼠深Ⅱ度烫伤创面中神经肽P物质(SP)表达及变化规律.方法 84只Wistar大鼠,随机分为糖尿病组和对照组,每组42只.糖尿病组腹腔内一次性注射链脲佐菌素(STZ)诱导建立糖尿病大鼠模型,对照组给予同等剂量的无菌柠檬酸缓冲液腹腔注射.所有大鼠麻醉后背部制成直径2 cm的深Ⅱ度烫伤创面.分别于烫伤前及烫伤后的不同时相采集创面组织标本,测算创面愈合率,免疫组化染色分析创面SP染色阳性区域面积,ELISA法创面组织中SP表达变化.结果烫伤后第7天起,糖尿病组各时相点的创面愈合率均显著低于对照组(P<0.01).糖尿病组各时相点创面SP染色阳性区域面积均显著低于对照组,其中以烫伤后7 d时尤为显著,为(1 350.93±99.28)μm2 vs(1 715.86±103.41)μm2(P<0.01);糖尿病组各时相创面组织中SP表达均明显低于对照组,其中以烫伤后7 d时最为显著,为(114.04±9.96)vs(143.39±8.94)(P<0.01).结论糖尿病大鼠创面组织中SP表达明显减少,可能是其创面难愈的原因之一.
목적 연구당뇨병대서심Ⅱ도탕상창면중신경태P물질(SP)표체급변화규률.방법 84지Wistar대서,수궤분위당뇨병조화대조조,매조42지.당뇨병조복강내일차성주사련뇨좌균소(STZ)유도건립당뇨병대서모형,대조조급여동등제량적무균저몽산완충액복강주사.소유대서마취후배부제성직경2 cm적심Ⅱ도탕상창면.분별우탕상전급탕상후적불동시상채집창면조직표본,측산창면유합솔,면역조화염색분석창면SP염색양성구역면적,ELISA법창면조직중SP표체변화.결과 탕상후제7천기,당뇨병조각시상점적창면유합솔균현저저우대조조(P<0.01).당뇨병조각시상점창면SP염색양성구역면적균현저저우대조조,기중이탕상후7 d시우위현저,위(1 350.93±99.28)μm2 vs(1 715.86±103.41)μm2(P<0.01);당뇨병조각시상창면조직중SP표체균명현저우대조조,기중이탕상후7 d시최위현저,위(114.04±9.96)vs(143.39±8.94)(P<0.01).결론 당뇨병대서창면조직중SP표체명현감소,가능시기창면난유적원인지일.
Objective To study the expression and change of neuropeptide substance P (SP) during the wound healing of deep partial thickness scalding in diabetic rats. Methods Eighty-four Wistar rats were randomly divided into diabetes mellitus group (n=42) and control group (n=42). Diabetic rat models were established by intraperitoneal injection of streptozotocin (STZ) in diabetes mellitus group, and those in control group were intraperitoneally injected with aseptic citrate buffer solution. Deep partial thickness scalding with diameter of 2 cm on the back were prepared in all the rats. The pre-scalding and post-scalding wound specimens of different time points were obtained, and the percentages of wound closure were calculated. The wound specimens were also obtained for immunohistological staining to compare the areas with positive staining of SP, and ELISA was employed to detect the expression of SP in the wound tissues. Results The percentage of wound closure was significantly lower in diabetes mellitus group than that in control group from 7 days post-scalding (P< 0.01). The areas with positive staining of SP in diabetes mellitus group were much smaller than those in control group at different time points, which was most significant on the seventh day post-scalding[(1 350.93±99.28) μm2 vs(1 715.86± 103.41) μm2](P < 0.01). The expression of SP in the wound tissues was significantly lower in diabetes mellitus group than that in control group at different time points, which was most significant on the seventh day post-scalding[(114.04±9.96) vs(143.39±8.94)](P<0.01). Conclusion The significantly lower expression of SP in wound site may be one of the causes of delayed wound healing in diabetic rats.