CAJ | 학술논문

采用戊二醛-牛血清蛋白(GA-BSA)交联法和聚碳酸酯膜直接固定法制备了大肠杆菌(E.coli)微生物电极,探讨了基于E.coil电极的微生物传感器在重金属和毒性有机物急性毒性分析中的应用性能.试验结果表明,采用戊二醛-牛血清蛋白交联固定法制备的E.coli微生物电极的灵敏性较差,难以满足毒性分析需要.聚碳酸酯膜直接固定法制备的E.coli微生物电极具有良好的灵敏性和稳定性,在冰箱4℃保存2个月,仍能很好地满足毒性分析需要,该固定方法具有制备简单和可实现电极的重复利用等特点,大幅降低了微生物传感器的毒性分析成本.
채용무이철-우혈청단백(GA-BSA)교련법화취탄산지막직접고정법제비료대장간균(E.coli)미생물전겁,탐토료기우E.coil전겁적미생물전감기재중금속화독성유궤물급성독성분석중적응용성능.시험결과표명,채용무이철-우혈청단백교련고정법제비적E.coli미생물전겁적령민성교차,난이만족독성분석수요.취탄산지막직접고정법제비적E.coli미생물전겁구유량호적령민성화은정성,재빙상4℃보존2개월,잉능흔호지만족독성분석수요,해고정방법구유제비간단화가실현전겁적중복이용등특점,대폭강저료미생물전감기적독성분석성본.
The bovine serum albumin-glutaraldehyde crosslinking process and polycarbonate membrane immobilization process were used to prepare E. coli microbial electrodes for toxicity microbial biosensor, and their performance for heavy metals and toxic organic compounds toxicity determination was studied. The results show that the sensitivity of the E. coli microbial electrodes prepared by GA - BSA immobilization method is very poor, and is not suitable for toxicity assessment. E. coli microbial electrode prepared by polycarbonate membrane immobilization process exhibits high sensitivity and good stability for toxicity determination, and is suitable for the toxicity assessment even after 2 months'storage at 4 ℃. The polycarbonate membrane immobilization process is very simple, and the reusing of electrodes can be realized and thus the cost of toxicity determination with microbial biosensor reduces.