CAJ | 학술논문

研究了注射促黄体激素类似物(LHRHa)后,泥鳅(Misgurnus anguillicaudatus)血清性类同醇激素的变化规律,并探讨在泥鳅繁殖季节时.孕酮(P)、睾酮(T)和雌二醇(E_2)对性腺发育的作用及调节机制.实验共分两组,对照组和实验组;对照组只注射生理盐水;实验组注射LHRHa,雌鱼0.2 μg/g,雄鱼减半.注射前尾静脉采血,作为血液样本分析基础水平(Oh),注射药物后分别在7、24、48、72和96h尾静脉采血.测定雌鱼睾酮、雌二醇和孕酮,雄鱼孕酮和睾酬血清浓度.实验结果表明:注射LHRHa,雌鱼血清睾酮和雌二醇浓度显著高于对照组,雄鱼血清睾酮和孕酮显著高于对照组(P<0.05);24h浓度较高.雌鱼孕酬、睾酮和雌二醇分别为(0.710±0.082)ng/mL、(9.00±0.57)ng/mL和(696.4±26.2)pg/mL,雄鱼孕酮和睾酮分别为(0.527±0.121)ng/mL和(9.62±0.62)ng/mL.实验组雌鱼孕酮变化基本规律为,基础水平(0-7h)-逐渐升至最高(7-24h)-逐渐降至基础水平(24-48h)-维持基础水平(48-96h).实验组雌鱼睾酮和雌二醇与雄鱼孕酮和睾酮变化规律基本相似,其规律为,逐渐上升至最高(0-24h)-逐渐降至基础水平(24-72h)-维持基础水(72-96h).24h对照组雌鱼睾酮和雌二醇显著升高,浓度分别为:睾酮(2.20±0.18)ng/mL,雌二醇(269.1±36.6)pg/mL.对照组雄鱼血清孕酮和睾酮浓度实验期间均无显著变化.研究认为:LHRHa能够刺激泥鳅性类同醇激素分泌,特别是睾酮的分泌,显著提高雌鱼性腺指数(GSI).但刺激P的分泌调控能力有限,实验期间处于较低水平,诱导排卵效果差,泥鳅的性类固醇激素可能有特殊的调节机制.雌二醇和睾酮对性腺成熟有重要作用,孕酮对介导卵细胞最终成熟和排卵可能起重要作用,而雌二醇和睾酮无明显效果.
연구료주사촉황체격소유사물(LHRHa)후,니추(Misgurnus anguillicaudatus)혈청성류동순격소적변화규률,병탐토재니추번식계절시.잉동(P)、고동(T)화자이순(E_2)대성선발육적작용급조절궤제.실험공분량조,대조조화실험조;대조조지주사생리염수;실험조주사LHRHa,자어0.2 μg/g,웅어감반.주사전미정맥채혈,작위혈액양본분석기출수평(Oh),주사약물후분별재7、24、48、72화96h미정맥채혈.측정자어고동、자이순화잉동,웅어잉동화고수혈청농도.실험결과표명:주사LHRHa,자어혈청고동화자이순농도현저고우대조조,웅어혈청고동화잉동현저고우대조조(P<0.05);24h농도교고.자어잉수、고동화자이순분별위(0.710±0.082)ng/mL、(9.00±0.57)ng/mL화(696.4±26.2)pg/mL,웅어잉동화고동분별위(0.527±0.121)ng/mL화(9.62±0.62)ng/mL.실험조자어잉동변화기본규률위,기출수평(0-7h)-축점승지최고(7-24h)-축점강지기출수평(24-48h)-유지기출수평(48-96h).실험조자어고동화자이순여웅어잉동화고동변화규률기본상사,기규률위,축점상승지최고(0-24h)-축점강지기출수평(24-72h)-유지기출수(72-96h).24h대조조자어고동화자이순현저승고,농도분별위:고동(2.20±0.18)ng/mL,자이순(269.1±36.6)pg/mL.대조조웅어혈청잉동화고동농도실험기간균무현저변화.연구인위:LHRHa능구자격니추성류동순격소분비,특별시고동적분비,현저제고자어성선지수(GSI).단자격P적분비조공능력유한,실험기간처우교저수평,유도배란효과차,니추적성류고순격소가능유특수적조절궤제.자이순화고동대성선성숙유중요작용,잉동대개도란세포최종성숙화배란가능기중요작용,이자이순화고동무명현효과.
This experiment was to clarify the change of sex steroid hormone in serum of loach (Misgurnus anguillicauda-tus) infected LHRHa, and investigate actions of progesterone (P) , testosterone (T) and estradiol (E_2) to gonadal development and regulatory mechanism. It contented two groups, control group and test group. The fish of control group were only injected physiological saline, and the fish of test group were injected chorionic gonadotropin analogue [ D-Ser( tBu)6, Pro9-NEt] ( LHRHa) (female fish 0.2ug/g and male fish 0. lug/g). Blood samples were collected from caudal vein at 0, 7, 24, 48, 72 and 96h after implantation respectively, and T, E_2 and P contents in serum were measured. These results showed that T and E_2 in serum of female fish infected LHRHa were significantly higher than those of control group (P < 0.05). 24h after infection LHRHa, P, T and E_2 contents of female fish were 0.710±0.082ng/mL, 9.00±0.57ng/mL and 696.4±26.2pg/mL respectively; P and T of male fish were 0.527±0.121ng/mL and 9. 62±0.62ng/mL respectively. Change discipline of female fish in test group was as following rule, basis level (0-7h)-to rise gradually to the peak value (7-24h) to go down to basis level (24-48h) to maintain basis level (48-96h). The change discipline of T and E_2in female fish and P and T in male fish were similar, to rise gradually to the peak value (0-24h) to go down gradually to basis level (24-48h) to maintain basis level (48-96h). After 24h, the T and E_2 contents of female fish in control group been significantly hoisted to 2.20+0.18ng/mL and 269.1+36.6pg /mL, but the P and T contents of male fish in control group changed unobviously ( P > 0.05). The experimental results indicated that LHRHa could stimulate sex steroid hormone secretion of loach to female loach, especially T, and raised significantly the gonadosomatic index (GSI) , but it had poor effect to induce ovulation. The reason may be that it had especial regulatory mechanism of steroid hormone. E_2 and T in loach could promote sex gland maturity. P may play important role in final maturity of oocyte and ovulation in loach, and E_2 and T had no obvious action in this way.