CAJ | 학술논문

目的探讨急性吗啡暴露对大鼠前额叶皮质τ蛋白磷酸化及学习记忆的影响.方法 40只雄性SD大鼠随机均分成急性吗啡暴露组(M组)和对照组(C组).M组单次腹腔注射吗啡(30 mg/kg),C组同法注射等量生理盐水.注射后2 h和24 h分别断头处死10只大鼠.免疫印迹技术检测前额叶皮质r蛋白的磷酸化水平.后处死的大鼠于注射后2、4及24 h分别行水迷宫实验.结果 M组注射后2 h时平台寻找时间显著长于C组(P<0.01).M组大鼠前额叶皮质τ蛋白Ser396和Thr231位点的磷酸化水平于注射后2 h时显著高于C组(P<0.05).结论急性吗啡暴露可致大鼠学习记忆功能可逆性减退,前额叶皮质r蛋白过度磷酸化可能与其有关.
목적 탐토급성마배폭로대대서전액협피질τ단백린산화급학습기억적영향.방법 40지웅성SD대서수궤균분성급성마배폭로조(M조)화대조조(C조).M조단차복강주사마배(30 mg/kg),C조동법주사등량생리염수.주사후2 h화24 h분별단두처사10지대서.면역인적기술검측전액협피질r단백적린산화수평.후처사적대서우주사후2、4급24 h분별행수미궁실험.결과 M조주사후2 h시평태심조시간현저장우C조(P<0.01).M조대서전액협피질τ단백Ser396화Thr231위점적린산화수평우주사후2 h시현저고우C조(P<0.05).결론 급성마배폭로가치대서학습기억공능가역성감퇴,전액협피질r단백과도린산화가능여기유관.
Objective To explore the effect of acute morphine on memory and phosphorylation of the prefrontal cortex of rats. Methods Forty SD rats were randomly divided into two groups with 20 rats each. Group M received intraperitoneally injection of morphine 30 mg/kg. Group C was given normal saline instead of morphine as the control. Ten rats from each group were sacrificed to get the prefrontal cortex for detecting τ protein phosphorylation at the sites Ser396 and Thr231 with immunoblotting at 2 h and 24 h after injections. Laterly sacrificed rats in each group were tested with morris water maze(MWM) at 2 h,4 h and 24 h after injections. Results The latent period was significantly longer in group M than that of group C(P<0. 01) at 2 h after injections. The hyperphosphorylation at the sites of Ser396 and Thr231 of τ in group M was significantly higher than that in group C (P< 0. 05) at 2 h after injection. Conclusion The intraperitoneally injection of morphine 30 mg/ kg may lead to transient memory decrease in rats, which may be related to the hyperphosphorylation at the sites of Ser396 and Thr231of τ in the prefrontal cortex.