CAJ | 학술논문

本研究以含不育基因Ms的大白菜细胞核复等位基因型雄性不育两用系"AB01"的可育株(MsfMs)为供体亲本,以高代自交系'a20'(msms)为轮回亲本,采用杂交和连续回交转育方法,利用与不育基因Ms连锁的SCAR标记syau_scr01辅助不育基因Ms选择,成功地将不育基因转育到可育品系‘a20'中,育成了不育度和不育株率均为100%,植物学性状与自交系‘a20'相近的新核不育系GMS4.选择结果表明syau_scr01选择的准确率为100%,验证了该标记可以用于大白菜核不育系转育辅助选择.
본연구이함불육기인Ms적대백채세포핵복등위기인형웅성불육량용계"AB01"적가육주(MsfMs)위공체친본,이고대자교계'a20'(msms)위륜회친본,채용잡교화련속회교전육방법,이용여불육기인Ms련쇄적SCAR표기syau_scr01보조불육기인Ms선택,성공지장불육기인전육도가육품계‘a20'중,육성료불육도화불육주솔균위100%,식물학성상여자교계‘a20'상근적신핵불육계GMS4.선택결과표명syau_scr01선택적준학솔위100%,험증료해표기가이용우대백채핵불육계전육보조선택.
In this research,the fertile plant (MsfMs) which contain the male sterility gene Ms of genetic multiple allele male sterile AB line "AB01" in Chinese cabbage was used to be as donor parent,and the inbred line 'a20' (msms) as recurrent parent,based on cross and continuous back-crossing.The SCAR marker,yau_scr01,which is closely linked to the male sterility gene Ms,was applied in the selection of Ms gene.We successfully transferred the male-sterile gene Ms into fertile line 'a20'.The resulting new male sterile line 'GMS4' is of 100% sterility frequency and sterility degree,which contains similar botanical characters with inbred line 'a20'.The selection results showed that the accuracy of marker syau_scr01 has reached 100%,which indicates that marker syau_scr01 could be applied in the marker-assisted selection of the genetic male sterile line in Chinese cabbage.