中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2008年
6期
445-448
,共4页
石瑞如%张国龙%苑雪芹%刘新%王国斌
石瑞如%張國龍%苑雪芹%劉新%王國斌
석서여%장국룡%원설근%류신%왕국빈
γ干扰素%分枝杆菌,结核%结核,肺%感染%抗原%诊断
γ榦擾素%分枝桿菌,結覈%結覈,肺%感染%抗原%診斷
γ간우소%분지간균,결핵%결핵,폐%감염%항원%진단
Interferon-gamma%Mycobacterium,tuberculosis%Tuberculosis,pulmonary%Infection%Antigen%Diagnosis
目的 探讨结核分枝杆菌特异性抗原早期分泌抗原靶6000(ESAT-6)体外诱发的γ干扰素反应在结核感染和结核病诊断中的价值.方法 外周全血中加入ESAT-6抗原,诱导产生γ干扰素,以酶联免疫吸附法测定γ干扰素浓度,以增加的百分率为ESAT-6抗原诱发的γ干扰素反应值.进行下列研究:对比结核密切接触者(60例)、痰培养证实的结核患者(46例)以及健康人(55例);追踪调查儿童(68例)、青少年(52例)、老年(45例);治疗过程中动态观察20例结核患者;比较HIV(+)TB(+)者(78例)和HIV(+)TB(-)者(60例).结果 结核密切接触者与痰培养证实的结核患者ESAT-6反应具有很高的敏感性;儿童与青少年ESAT-6反应与临床诊断和预后具有很高的一致性;65%的结核患者在治疗过程中ESAT-6反应呈下降趋势;ESAT-6反应在HIV(+)TB(+)的诊断中敏感度为87.2%.结论 体外ESAT-6诱发的γ干扰素测定对活动性结核病尤其是儿童和青少年结核病的诊断有一定意义,也可用于鉴别HIV阳性者合并结核.
目的 探討結覈分枝桿菌特異性抗原早期分泌抗原靶6000(ESAT-6)體外誘髮的γ榦擾素反應在結覈感染和結覈病診斷中的價值.方法 外週全血中加入ESAT-6抗原,誘導產生γ榦擾素,以酶聯免疫吸附法測定γ榦擾素濃度,以增加的百分率為ESAT-6抗原誘髮的γ榦擾素反應值.進行下列研究:對比結覈密切接觸者(60例)、痰培養證實的結覈患者(46例)以及健康人(55例);追蹤調查兒童(68例)、青少年(52例)、老年(45例);治療過程中動態觀察20例結覈患者;比較HIV(+)TB(+)者(78例)和HIV(+)TB(-)者(60例).結果 結覈密切接觸者與痰培養證實的結覈患者ESAT-6反應具有很高的敏感性;兒童與青少年ESAT-6反應與臨床診斷和預後具有很高的一緻性;65%的結覈患者在治療過程中ESAT-6反應呈下降趨勢;ESAT-6反應在HIV(+)TB(+)的診斷中敏感度為87.2%.結論 體外ESAT-6誘髮的γ榦擾素測定對活動性結覈病尤其是兒童和青少年結覈病的診斷有一定意義,也可用于鑒彆HIV暘性者閤併結覈.
목적 탐토결핵분지간균특이성항원조기분비항원파6000(ESAT-6)체외유발적γ간우소반응재결핵감염화결핵병진단중적개치.방법 외주전혈중가입ESAT-6항원,유도산생γ간우소,이매련면역흡부법측정γ간우소농도,이증가적백분솔위ESAT-6항원유발적γ간우소반응치.진행하렬연구:대비결핵밀절접촉자(60례)、담배양증실적결핵환자(46례)이급건강인(55례);추종조사인동(68례)、청소년(52례)、노년(45례);치료과정중동태관찰20례결핵환자;비교HIV(+)TB(+)자(78례)화HIV(+)TB(-)자(60례).결과 결핵밀절접촉자여담배양증실적결핵환자ESAT-6반응구유흔고적민감성;인동여청소년ESAT-6반응여림상진단화예후구유흔고적일치성;65%적결핵환자재치료과정중ESAT-6반응정하강추세;ESAT-6반응재HIV(+)TB(+)적진단중민감도위87.2%.결론 체외ESAT-6유발적γ간우소측정대활동성결핵병우기시인동화청소년결핵병적진단유일정의의,야가용우감별HIV양성자합병결핵.
Objective To evaluate the value of early secreted antigen terget 6000(ESAT-6)-induced interferon-gamma(IFN-γ)response in vitro in diagnosis of tuberculosis and mycobacterium tuberculosis infections.Methods ESAT-6 was added into peripheral whole blood to induce IFN-γ,and concentration of IFN-γ was detected by ELISA.Comparing with saline control,the percentage of increase was regarded as the value of ESAT-6 induced IFN-γ response.ESAT-6-induced IFN-γ response was analysed in the following four aspects:to compare with the people intimate contact to tuberculosis(60 cases),the sputum culture confirmed active tuberculosis patients(46 eases)and healthy people(55 cases);to compare with children(68 cases),adolescents(52 cases)and elder people(45 cases)by follow up;dynamically observe 20 tuberculosis patients during long-time treatment;to compare HIV(+)and TB(+)people(78 cases)with HIV(+)and TB(-)people(60 cases).Results Between people intimate contacts to tuberculosis and active tuberculosis patients,ESAT-6 response has high sensitivity;ESAT-6 response in children and adolescents was completely consistent with the following clinical diagnosis and prognosis;ESAT-6 response decreased during treatment duration in 65%tuberculosis patients;in HIV(+)and TB(+)cases,the sensitivity of tuberculosis diagnosis by ESAT-6 response was 87.2%.Conclusion In vitro IFN-γ response induced by ESAT-6 is helpful in diagnosis of tuberculosis,especially for children and adolescents.And it is also available for differential diagnosis of HIV(+)patients with tuberculosis.
