中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
6期
401-404
,共4页
宾萍%冷曙光%程娟%段化伟%潘祖飞%戴宇飞%牛勇%刘清君%陈泓%刘庆%郑玉新
賓萍%冷曙光%程娟%段化偉%潘祖飛%戴宇飛%牛勇%劉清君%陳泓%劉慶%鄭玉新
빈평%랭서광%정연%단화위%반조비%대우비%우용%류청군%진홍%류경%정옥신
多环碳氢化合物%环AMP受体基因%多态性,单核苷酸%端粒长度
多環碳氫化閤物%環AMP受體基因%多態性,單覈苷痠%耑粒長度
다배탄경화합물%배AMP수체기인%다태성,단핵감산%단립장도
Polycyclic hydrocarbons%Cyclic AMP receptor%Gene polymorphisms,single nucleotide%Telomere length
目的 探讨多环芳烃(polycyclic aromatic hydrocarbons,PAHs)接触工人外周血全基因组DNA的端粒长度与PAHs代谢相关基因多态性的关系.方法 选取145名焦炉作业工人(接触组)和68名无职业性PAHs及射线接触的医务人员(对照组)作为调查对象.测定尿中1-羟基芘(1-hydroxypyrene,1-OHP)水平反映PAHs接触内剂量,采用实时定量PCR的方法,测定研究对象外周血全基因组DNA的相对端粒长度(RTL),采用基于PCR的方法进行PAHs代谢相关基因多态性分析.结果 与对照组(1.43+1.06)相比,PAHs接触组的RTL(1.10±0.75)明显缩短,差异有统计学意义(P=0.026).校正了性别、年龄、每日吸烟量和尿中1-OHP后,接触组CYP1A1基因3801 T>C位点CT基因型个体RTL(1.25±0.93)长于TT基因型(0.93±0.51),差异有统计学意义(P=0.043);mEH基因Tyr113His位点Tyr/His基因型个体RTL(0.90±0.58)短于Tyr/Tyr基因型(1.24±0.90),差异有统计学意义(P=0.043);AHR基因rs10250822位点CT基因型个体RTL(1.02±0.64)短于CC基因型个体(1.36±1.14),差异有统计学意义(P=0.044);AHR基因m10247158位点AT基因型个体RTL(0.93±0.54)短于AA基因型(1.19±0.84),差异有统计学意义(P=0.047).结论 接触PAHs可以引起接触工人外周血全基因组DNA RTL缩短,在PAHs接触工人中,CYP1A1、mEH、AHR基因多态性可能是影响外周血全基因组DNA RTL的遗传因素之一.
目的 探討多環芳烴(polycyclic aromatic hydrocarbons,PAHs)接觸工人外週血全基因組DNA的耑粒長度與PAHs代謝相關基因多態性的關繫.方法 選取145名焦爐作業工人(接觸組)和68名無職業性PAHs及射線接觸的醫務人員(對照組)作為調查對象.測定尿中1-羥基芘(1-hydroxypyrene,1-OHP)水平反映PAHs接觸內劑量,採用實時定量PCR的方法,測定研究對象外週血全基因組DNA的相對耑粒長度(RTL),採用基于PCR的方法進行PAHs代謝相關基因多態性分析.結果 與對照組(1.43+1.06)相比,PAHs接觸組的RTL(1.10±0.75)明顯縮短,差異有統計學意義(P=0.026).校正瞭性彆、年齡、每日吸煙量和尿中1-OHP後,接觸組CYP1A1基因3801 T>C位點CT基因型箇體RTL(1.25±0.93)長于TT基因型(0.93±0.51),差異有統計學意義(P=0.043);mEH基因Tyr113His位點Tyr/His基因型箇體RTL(0.90±0.58)短于Tyr/Tyr基因型(1.24±0.90),差異有統計學意義(P=0.043);AHR基因rs10250822位點CT基因型箇體RTL(1.02±0.64)短于CC基因型箇體(1.36±1.14),差異有統計學意義(P=0.044);AHR基因m10247158位點AT基因型箇體RTL(0.93±0.54)短于AA基因型(1.19±0.84),差異有統計學意義(P=0.047).結論 接觸PAHs可以引起接觸工人外週血全基因組DNA RTL縮短,在PAHs接觸工人中,CYP1A1、mEH、AHR基因多態性可能是影響外週血全基因組DNA RTL的遺傳因素之一.
목적 탐토다배방경(polycyclic aromatic hydrocarbons,PAHs)접촉공인외주혈전기인조DNA적단립장도여PAHs대사상관기인다태성적관계.방법 선취145명초로작업공인(접촉조)화68명무직업성PAHs급사선접촉적의무인원(대조조)작위조사대상.측정뇨중1-간기비(1-hydroxypyrene,1-OHP)수평반영PAHs접촉내제량,채용실시정량PCR적방법,측정연구대상외주혈전기인조DNA적상대단립장도(RTL),채용기우PCR적방법진행PAHs대사상관기인다태성분석.결과 여대조조(1.43+1.06)상비,PAHs접촉조적RTL(1.10±0.75)명현축단,차이유통계학의의(P=0.026).교정료성별、년령、매일흡연량화뇨중1-OHP후,접촉조CYP1A1기인3801 T>C위점CT기인형개체RTL(1.25±0.93)장우TT기인형(0.93±0.51),차이유통계학의의(P=0.043);mEH기인Tyr113His위점Tyr/His기인형개체RTL(0.90±0.58)단우Tyr/Tyr기인형(1.24±0.90),차이유통계학의의(P=0.043);AHR기인rs10250822위점CT기인형개체RTL(1.02±0.64)단우CC기인형개체(1.36±1.14),차이유통계학의의(P=0.044);AHR기인m10247158위점AT기인형개체RTL(0.93±0.54)단우AA기인형(1.19±0.84),차이유통계학의의(P=0.047).결론 접촉PAHs가이인기접촉공인외주혈전기인조DNA RTL축단,재PAHs접촉공인중,CYP1A1、mEH、AHR기인다태성가능시영향외주혈전기인조DNA RTL적유전인소지일.
Objective To investigate the association between the polymorphisms of metabolic genes and telomere length of genomic DNA in peripheral blood of workers exposed to polycyclic aromatic hydrocarbons (PAHs).Methods One hundred and forty five coke-oven workers exposed to PAHs and sixty eight non-exposed medical staffs were recruited in this study.Urinary 1-hydroxypyrene (1-OHP) served as the internal exposure dose of PAHs for all subjects.Relative telomere length (RTL) of genomic DNA in peripheral blood was used as telomere length and measured by real-time PCR.Polymorphisms of metabolic genes were detected by PCR-based methods.Results Compared with control group,the exposure group shown a decreased RTL (1.10±0.75 vs 1.43±1.06,P<0.05).In the coke-oven workers,after adjusting the sex,age,cigarettes per day and urinary 1-OHP,RTL (1.25±0.93) of workers with CT genotype at the CYP1A1 3801 T>C was significantly longer than that (0.93±0.51) of workers with TT genotype (P<0.05).RTL (0.90±0.58) of individuals with the Tyr/His genotype at mEH Tyr113His was significantly shorter than that (1.24±0.90) of individuals with the Tyr/Tyr genotype (P<0.05).RTL (1.02±0.64) of individuals with the CT genotype at AHR rs 10250822 was significantly shorter than that (1.36+1.14) of individuals with the CC genotype (P<0.05 ).RTL (0.93±0.54) of individuals with the AT genotype at AHR rs10247158 was significantly shorter than that (1.19±0.84) of individuals with the AA genotype (P<0.05).Conclusion The results of present study suggested that PAHs exposure could induce the shorted RTL,CYP1A1,mEH,AHR polymorphisms might influence the change of telomere length of genomic DNA in peripheral blood of workers exposed to PAHs.
