CAJ | 학술논문

目的观测抑郁症患者免疫失衡的特征,从5-羟色胺受体(5-HT1aR)和调节性T 细胞表达变化的关系,探讨抑郁症免疫失衡的可能机制.方法采集27例抑郁症患者外周血,利用ELISA方法测定血清细胞因子IL-2,IL-10,TGF-β1浓度,逆转录-聚合酶链反应(RT-PCR)方法检测患者5-HT1aR和FoxP3的mRNA水平,免疫磁珠分离调节性T细胞,共聚焦显微镜观察调节性T细胞5-HT1aR受体和FoxP3的共表达,并与正常对照组进行对照.结果与正常对照组标本相比,抑郁症患者血清IL-2表达水平上调[(184.681±8.472)pg/ml,(82.845±12.292)pg/ml],IL-10[(6.765±0.611)pg/ml,(9.593±0.921)pg/ml],TGF-β1[(14.042±2.170)ng/ml,(20.981±3.98)ng/ml]的表达水平降低(均P <0.01);外周血中调节性T细胞数量减少[(13.139±4.587)107个,(20.583±3.484)107个],单个核细胞5-HT1aR和FoxP3的mRNA表达水平降低(均P <0.01);同时通过共聚焦显微镜观察到调节T细胞上的5-HT1aR及FoxP3表达减弱.结论抑郁症患者中存在免疫失衡现象,5-HT1aR通过影响调节性T细胞在抑郁症患者免疫失衡的病理生理机制发挥着重要的作用.
목적 관측억욱증환자면역실형적특정,종5-간색알수체(5-HT1aR)화조절성T 세포표체변화적관계,탐토억욱증면역실형적가능궤제.방법 채집27례억욱증환자외주혈,이용ELISA방법측정혈청세포인자IL-2,IL-10,TGF-β1농도,역전록-취합매련반응(RT-PCR)방법검측환자5-HT1aR화FoxP3적mRNA수평,면역자주분리조절성T세포,공취초현미경관찰조절성T세포5-HT1aR수체화FoxP3적공표체,병여정상대조조진행대조.결과 여정상대조조표본상비,억욱증환자혈청IL-2표체수평상조[(184.681±8.472)pg/ml,(82.845±12.292)pg/ml],IL-10[(6.765±0.611)pg/ml,(9.593±0.921)pg/ml],TGF-β1[(14.042±2.170)ng/ml,(20.981±3.98)ng/ml]적표체수평강저(균P <0.01);외주혈중조절성T세포수량감소[(13.139±4.587)107개,(20.583±3.484)107개],단개핵세포5-HT1aR화FoxP3적mRNA표체수평강저(균P <0.01);동시통과공취초현미경관찰도조절T세포상적5-HT1aR급FoxP3표체감약.결론 억욱증환자중존재면역실형현상,5-HT1aR통과영향조절성T세포재억욱증환자면역실형적병리생리궤제발휘착중요적작용.
Objective To observe the immune imbalance in depression patients,as well as to explore the role of 5-HT,5-HT1a receptor(5-HT1aR) and the regular T cell in the pathological imbalance mechanism of depression disorder.Methods 27 depression patients were selected with the criteria as CCMD-3 and DSM-Ⅳ. The concentration of serum IL-2,IL-10,TGF-β1 were detected by ELISA, the serum 5-HT concentration was measured by high performance liquid chromatogram, the 5-HT1aR and FoxP3 mRNA expression level are obtained by RT-PCR, CD4+CD25+ T regular cells were detached from PBMCs by immunomagnetic beads and the coexpression of the 5-HT1aR and FoxP3 in regular T cells are observed by Confocal Microscope.Results The serum concentration of IL-10(6.765±0.611)pg/ml VS (9.593±0.921)pg/ml and TGF-β1(6.765±0.611)pg/ml VS (9.593±0.921)pg/ml were decreased while the IL-2(184.681±8.472)pg/ml VS (82.845±12.292)pg/ml was increased in patients compared with those of controls ( P <0.05). Both 5-HT1aR and FoxP3 mRNA expression in immune cells and the proportion of regular T cells were decreased in patients, meanwhile the 5-HT1aR and FoxP3 expression in regular T cells were also specially declined when observed by immunofluorescence method.Conclusion The results of this study support the hypothesis that the 5-HT plays a role in the immune imbalance of depression, probably through the regular T cell pathway via 5-HT1aR.