CAJ | 학술논문

目的探讨磷酸酶和张力蛋白同源基因(PTEN)及磷脂酰肌醇3激酶(PI3K)在直肠癌中的表达与临床病理特征的相关性.方法应用免疫组织化学染色MaxVisionTM法检测60例直肠癌、30例直肠腺瘤和10例正常直肠黏膜组织标本中PTEN及PI3K的表达情况,并对其表达水平与直肠癌临床病理特征之间的关系进行相关性分析.结果直肠癌组织PTEN阳性表达率明显低于正常直肠黏膜组织和直肠腺瘤组织[43.3％( 26/60)比100.0％(10/10)和93.3％( 28/30),P＜0.05],直肠癌组织PI3K阳性表达率明显高于正常直肠黏膜组织和直肠腺瘤组织[75.0％( 45/60)比30.0％(3/10)和33.3％(10/30),P＜0.05].PTEN及PI3K在直肠癌组织中的表达水平与患者术前癌胚抗原、分化程度、TNM分期、淋巴结转移和远处转移有关(P＜0.01或＜0.05),而与患者年龄、性别、肿瘤位置、肿瘤形态无明显相关性(P＞0.05).相关分析结果表明,PI3K与PTEN阳性表达呈显著负相关(r=-0.269,P=0.023).结论直肠癌中PI3K高表达及PTEN低表达,与直肠癌的侵袭和转移密切相关,监测两者的表达对判定直肠癌生物学行为有一定的价值.
목적 탐토린산매화장력단백동원기인(PTEN)급린지선기순3격매(PI3K)재직장암중적표체여림상병리특정적상관성.방법 응용면역조직화학염색MaxVisionTM법검측60례직장암、30례직장선류화10례정상직장점막조직표본중PTEN급PI3K적표체정황,병대기표체수평여직장암림상병리특정지간적관계진행상관성분석.결과 직장암조직PTEN양성표체솔명현저우정상직장점막조직화직장선류조직[43.3％( 26/60)비100.0％(10/10)화93.3％( 28/30),P＜0.05],직장암조직PI3K양성표체솔명현고우정상직장점막조직화직장선류조직[75.0％( 45/60)비30.0％(3/10)화33.3％(10/30),P＜0.05].PTEN급PI3K재직장암조직중적표체수평여환자술전암배항원、분화정도、TNM분기、림파결전이화원처전이유관(P＜0.01혹＜0.05),이여환자년령、성별、종류위치、종류형태무명현상관성(P＞0.05).상관분석결과표명,PI3K여PTEN양성표체정현저부상관(r=-0.269,P=0.023).결론 직장암중PI3K고표체급PTEN저표체,여직장암적침습화전이밀절상관,감측량자적표체대판정직장암생물학행위유일정적개치.
Objective To investigate the expression of phosphatase and tensin homolog gene (PTEN) and 1-phosphatidylinositol 3-kinase (PI3K) in rectal cancer and its correlation with clinical pathology.Methods MaxVisionTM immunohistochemical method was used to detect the expressions of PTEN and PI3K in 60 rectal cancer tissues,30 adenoma tissues and 10 normal rectal tissues,and the correlation between the expression levels and its clinicopathologic characteristics was discussed.Results The positive expression rate of PTEN in rectal cancer tissue was significantly lower than that in normal rectal tissue and adenoma tissue [43.3％ (26/60) vs.100.0％ (10/10),93.3％ (28/30),P ＜ 0.05].The positive expression rate of PI3K in rectal cancer tissue was significantly higher than that in normal rectal tissue and adenoma tissue [75.0％ (45/60) vs.30.0％ (3/10),33.3％ (10/30),P ＜ 0.05].The positive expression of PTEN and PI3K were correlated with carcinoembryonic antigen,degree of cell differentiation,TNM stage,lymph node metastasis and distant metastasis(P＜ 0.01 or ＜ 0.05 ),but had no correlation with age,gender,neoplasm location,neoplasm appearance (P ＞ 0.05).The correlated analysis showed that the positive expression of PTEN had negative correlation with PI3K (r =-0.269,P =0.023 ).Conclusions The high expression of PI3K and down regulation of PTEN in rectal cancer are closely related to its invasion and metastasis.There are some values to monitor PTEN and PI3K expression for determining biological behavior of rectal cancer.