CAJ | 학술논문

目的观察水通道蛋白1(AQP1)、水通道蛋白3(AQP3)在供体鼠肺移植热缺血损伤过程中的表达,探讨其表达与肺水肿的关系.方法 30只SD大鼠按肺热缺血时间随机分为3组:A组:0 h(n=10);B组:1 h(n=10);C组:2 h(n=10).免疫组织化学、Western blot和逆转录-聚合酶链反应(RT-PCR)法检测肺组织AQP1、AQP3蛋白和mRNA的表达.结果免疫组织化学显示,A、B、C 3组均有AQP1,AQP3蛋白表达.3组中AQP1 mRNA表达A值分别为0.63±0.10、0.49±0.07、0.36±0.06,AQP3分别为0.85±0.06、0.76±0.08、0.52±0.07.3组中AQP1蛋白相对灰度值分别为1.13±0.18、0.88±0.13、0.76±0.09,AQP3分别为1.92±0.26、0.95±0.15、0.61±0.07,差异均有统计学意义(P<0.05).结论肺移植过程中热缺血损伤造成AQP1、AQP3蛋白表达下调和肺水肿的发生;手术应尽量缩短热缺血时间.
목적 관찰수통도단백1(AQP1)、수통도단백3(AQP3)재공체서폐이식열결혈손상과정중적표체,탐토기표체여폐수종적관계.방법 30지SD대서안폐열결혈시간수궤분위3조:A조:0 h(n=10);B조:1 h(n=10);C조:2 h(n=10).면역조직화학、Western blot화역전록-취합매련반응(RT-PCR)법검측폐조직AQP1、AQP3단백화mRNA적표체.결과 면역조직화학현시,A、B、C 3조균유AQP1,AQP3단백표체.3조중AQP1 mRNA표체A치분별위0.63±0.10、0.49±0.07、0.36±0.06,AQP3분별위0.85±0.06、0.76±0.08、0.52±0.07.3조중AQP1단백상대회도치분별위1.13±0.18、0.88±0.13、0.76±0.09,AQP3분별위1.92±0.26、0.95±0.15、0.61±0.07,차이균유통계학의의(P<0.05).결론 폐이식과정중열결혈손상조성AQP1、AQP3단백표체하조화폐수종적발생;수술응진량축단열결혈시간.
Objective To explore the expression of aquaporin1 and aquaporin3 in the donor lung with warm ischemia, and study whether the,expression level of aquaporinl and aquaporin3 is related to the acute lung injury caused by warm ischemia. Methods Adult Wistar rats were divided into 3 groups ac-cording to the warm ischemic time of 0 h (group A,n = 10) ,1 h (group B,n = 10) ,and 2 h (group C, n = 10). We examined the expression level of AQP1 and AQP3 protein and mRNA in the lung tissues by immunohistochemistry, Western blotting, and reverse transcriptase-polymerase chain reaction. Results Immunohistochemical examination revealed that AQP1 and AQP3 were expressed in 3 groups. The mRNA average A values of AQP1 were 0.63±0. 10,0.49±0.07, and 0.36±0. 06, and those of AQP3 were 0.85 ± 0. 06,0.76 ± 0.08, and 0. 52± 0. 07 in groups A, B, and C, respectively. The protein average A values of AQP1 were 1.13±0.18,0.88 ± 0.13, and 0.76±0.09, and those AQP3 were 1.92 ± 0. 26. 0. 95± 0. 15, and 0.61±0. 07 in groups A, B, and C, respectively. Conclusion We conclude that AQP1 and AQP3 may play an important role in the maintenance of water homeostasis and are related to water movement and acute lung injury caused by warm ischemia.