中华手外科杂志
中華手外科雜誌
중화수외과잡지
CHINESE JOURNAL OF HAND SURGERY
2011年
2期
77-80
,共4页
方有生%刘杰%黄芳%顾玉东
方有生%劉傑%黃芳%顧玉東
방유생%류걸%황방%고옥동
坐骨神经%神经再生%模型,动物%人羊膜上皮细胞
坐骨神經%神經再生%模型,動物%人羊膜上皮細胞
좌골신경%신경재생%모형,동물%인양막상피세포
Sciatic nerve%Nerve regeneration%Models,animal%Human amniotic epithelial cells
目的 通过实验研究了解人羊膜上皮细胞(human amniotic epithelial cells,HAECs)对大鼠坐骨神经再生的促进作用.方法 取60只SD大鼠随机分为两组,羊膜细胞组和对照组,各组再分为2周和4周组,每组15只.制作大鼠周围神经损伤再生室模型,HAECs组局部应用培养的HAECs,对照组局部使用等量的生理盐水.术后分别于2周、4周检测神经传导功能和HE染色观察神经纤维形态学变化.结果 术后2周两组的神经电生理及组织学检测比较差异无统计学意义;4周HAECs组的大鼠坐骨神经传导功能明显恢复,HE染色显示术后坐骨神经手术部位出现大量炎性肉芽组织,呈现纤维性修复,吻合口以远HAECs组神经纤维形态结构较对照组完整,神经纤维与髓鞘直径均较对照组大.结论 HAECs移植可加速大鼠坐骨神经损伤后神经传导功能恢复,有助于有髓神经纤维损伤后的轴突与髓鞘的再生修复.
目的 通過實驗研究瞭解人羊膜上皮細胞(human amniotic epithelial cells,HAECs)對大鼠坐骨神經再生的促進作用.方法 取60隻SD大鼠隨機分為兩組,羊膜細胞組和對照組,各組再分為2週和4週組,每組15隻.製作大鼠週圍神經損傷再生室模型,HAECs組跼部應用培養的HAECs,對照組跼部使用等量的生理鹽水.術後分彆于2週、4週檢測神經傳導功能和HE染色觀察神經纖維形態學變化.結果 術後2週兩組的神經電生理及組織學檢測比較差異無統計學意義;4週HAECs組的大鼠坐骨神經傳導功能明顯恢複,HE染色顯示術後坐骨神經手術部位齣現大量炎性肉芽組織,呈現纖維性脩複,吻閤口以遠HAECs組神經纖維形態結構較對照組完整,神經纖維與髓鞘直徑均較對照組大.結論 HAECs移植可加速大鼠坐骨神經損傷後神經傳導功能恢複,有助于有髓神經纖維損傷後的軸突與髓鞘的再生脩複.
목적 통과실험연구료해인양막상피세포(human amniotic epithelial cells,HAECs)대대서좌골신경재생적촉진작용.방법 취60지SD대서수궤분위량조,양막세포조화대조조,각조재분위2주화4주조,매조15지.제작대서주위신경손상재생실모형,HAECs조국부응용배양적HAECs,대조조국부사용등량적생리염수.술후분별우2주、4주검측신경전도공능화HE염색관찰신경섬유형태학변화.결과 술후2주량조적신경전생리급조직학검측비교차이무통계학의의;4주HAECs조적대서좌골신경전도공능명현회복,HE염색현시술후좌골신경수술부위출현대량염성육아조직,정현섬유성수복,문합구이원HAECs조신경섬유형태결구교대조조완정,신경섬유여수초직경균교대조조대.결론 HAECs이식가가속대서좌골신경손상후신경전도공능회복,유조우유수신경섬유손상후적축돌여수초적재생수복.
Objective To study the effects of human amniotc epithelial cells (HAECs) on regeneration of rat sciatic nerve. Methods Sixty SD rats were randomly divided into two groups, the HAECs group and control group. Each group was further divided into two week group and four week group, with 15 rats each. The sciatic nerve was cut and the regeneration chamber was created. The HAECs were implanted into the regeneration chamber in the HAECs group and normal saline was injected into the regeneration chamber in the control group.Electro-physiological and the histological study was done at two weeks and four weeks after the surgery. The results of nerve conduction study and HE staining of regenerating nerve fibers were analyzed statistically.Results There were no statistically significant differences between the two groups at two weeks post-operatively.At four weeks, the experimental group showed significantly higher nerve conduction velocity and amplitude of evoked potentials comparing to those of the control group. Nerve fibers and inflammatory granulation tissue was seen near the lesion site by HE staining in the control group, whereas the HAECs group showed more integrated nerve fihers and mature myelination distal to the lesion site. Conclusion The application of human amniotic epithelial cells may enhance nerve regeneration in rats.
