中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2009年
5期
303-306
,共4页
包红雨%江淼%马珍妮%沈飞%朱明清%陈琳%谢丽倩%董宁征%阮长耿
包紅雨%江淼%馬珍妮%瀋飛%硃明清%陳琳%謝麗倩%董寧徵%阮長耿
포홍우%강묘%마진니%침비%주명청%진림%사려천%동저정%원장경
RNA干扰%基因,Annexin Ⅱ%Jurkat细胞%细胞增殖%趋化作用
RNA榦擾%基因,Annexin Ⅱ%Jurkat細胞%細胞增殖%趨化作用
RNA간우%기인,Annexin Ⅱ%Jurkat세포%세포증식%추화작용
RNA interference%Gene,annexin Ⅱ%Jurkat cells%Cell proliferation%Invasivepotential
目的 观察沉默Annexin Ⅱ(AnxA2)基因对淋巴瘤细胞系Jurkat细胞增殖、趋化能力的影响.方法 应用实时荧光定量RT-PCR和流式细胞术鉴定小干扰RNA(siRNA)对人淋巴瘤细胞系Jurkat细胞的转染效果,应用MTT法检测AnxA2 siRNA对Jurkat细胞增殖的作用,应用Transwell检测对细胞趋化能力的影响.结果 AnxA2 siRNA干扰组在24、48、72 h细胞生长抑制率分别为(17.4±2.3)%、(22.4±3.8)%和(37.6±1.5)%,与阴性对照组[分别为(-1.3±5.1)%、(-5.5±4.4)%和(-10.8±5.5)%]比较差异有统计学意义(P<0.05),AnxA2 siRNA干扰对Jurkat细胞增殖起抑制作用;AnxA2 siRNA干扰组细胞趋化能力(11.3±4.2)显著低于阴性对照组(54.3±8.7,P<0.01),AnxA2 siRNA干扰可显著降低细胞趋化能力.结论 siRNA沉默AnxA2基因可抑制Jurkat细胞增殖、趋化能力.
目的 觀察沉默Annexin Ⅱ(AnxA2)基因對淋巴瘤細胞繫Jurkat細胞增殖、趨化能力的影響.方法 應用實時熒光定量RT-PCR和流式細胞術鑒定小榦擾RNA(siRNA)對人淋巴瘤細胞繫Jurkat細胞的轉染效果,應用MTT法檢測AnxA2 siRNA對Jurkat細胞增殖的作用,應用Transwell檢測對細胞趨化能力的影響.結果 AnxA2 siRNA榦擾組在24、48、72 h細胞生長抑製率分彆為(17.4±2.3)%、(22.4±3.8)%和(37.6±1.5)%,與陰性對照組[分彆為(-1.3±5.1)%、(-5.5±4.4)%和(-10.8±5.5)%]比較差異有統計學意義(P<0.05),AnxA2 siRNA榦擾對Jurkat細胞增殖起抑製作用;AnxA2 siRNA榦擾組細胞趨化能力(11.3±4.2)顯著低于陰性對照組(54.3±8.7,P<0.01),AnxA2 siRNA榦擾可顯著降低細胞趨化能力.結論 siRNA沉默AnxA2基因可抑製Jurkat細胞增殖、趨化能力.
목적 관찰침묵Annexin Ⅱ(AnxA2)기인대림파류세포계Jurkat세포증식、추화능력적영향.방법 응용실시형광정량RT-PCR화류식세포술감정소간우RNA(siRNA)대인림파류세포계Jurkat세포적전염효과,응용MTT법검측AnxA2 siRNA대Jurkat세포증식적작용,응용Transwell검측대세포추화능력적영향.결과 AnxA2 siRNA간우조재24、48、72 h세포생장억제솔분별위(17.4±2.3)%、(22.4±3.8)%화(37.6±1.5)%,여음성대조조[분별위(-1.3±5.1)%、(-5.5±4.4)%화(-10.8±5.5)%]비교차이유통계학의의(P<0.05),AnxA2 siRNA간우대Jurkat세포증식기억제작용;AnxA2 siRNA간우조세포추화능력(11.3±4.2)현저저우음성대조조(54.3±8.7,P<0.01),AnxA2 siRNA간우가현저강저세포추화능력.결론 siRNA침묵AnxA2기인가억제Jurkat세포증식、추화능력.
Objective To investigate the effects of Annexin Ⅱ (AnxA2) gene silencing by siRNA on proliferation and invasive potential of lymphoma cell line Jurkat cells. Methods A synthesized siRNA duplex targeting to AnxA2 was transfected into Jurkat cells. Transfection efficiency was analyzed by real-time PCR and flow cytometry. MTF assay for cell proliferation and transwell plates for invasive potential were performed. Results Compared with the negative controls, the cell proliferation inhibitory rate of the AnxA2 siRNA trans- fected Jurkat cells was significantly increased at 24 h, 48 h and 72 h [(17.4 ± 2.3) %, (22.4 ± 3.8) %, (37.6 ± 1.5)% vs (- 1.3±5.1)%, (-5.5±4.4)%, (- 10.8± 5.5)%, respectively, P <0.05]. The cell invasive potential of the transfected Jurkat cells was inhibited remarkably at 48 h (11.3 ± 4.2 vs 54.3 ± 8.7, P <0.01). Conclusion AnxA2 gene silenced by siRNA can inhibit the proliferation and the invasive potential of Jurkat cells remarkably.
