CAJ | 학술논문

背景:冠状动脉微血管阻塞在急性冠状动脉综合征时相当常见,并且对预后产生不利影响.设计:随机对照的实验研究.目的:建立大鼠冠状动脉微血栓模型,为进一步研究冠状动脉微血管阻塞的病理生理意义奠定基础.地点、材料和干预:实验在福建医科大学附属协和医院鼠类动物实验室完成.实验选取24只SPF级雄性成年SD大鼠,按随机数字表法将大鼠分为实验组和对照组,每组各12只.钳夹升主动脉后自主动脉根部注入月桂酸钠1.0 mg/kg,1.5 h后取标本.主要观察指标:注入月桂酸钠后大鼠冠状动脉微血管内皮损害及微血栓形成情况;早期缺氧心肌情况.结果:①注入月桂酸钠1.5h后冠状动脉微血栓组所有大鼠均出现微动脉血管内皮损伤及微动脉血栓形成,对照组无此情况(P＜0.01);冠状动脉微血栓组微动脉内皮损伤率为(10.17±2.33)%,血栓形成率为(9.42±2.02)%.②Nagar-Olsen染色显示心肌缺血区域占所观察心肌横截面面积的(7.52±2.26)%,对照组无心肌缺血(P＜0.01).结论:月桂酸钠可稳定地诱发冠状动脉微血栓形成,此模型可作为冠状动脉微血栓及微栓塞研究的平台.
배경:관상동맥미혈관조새재급성관상동맥종합정시상당상견,병차대예후산생불리영향.설계:수궤대조적실험연구.목적:건립대서관상동맥미혈전모형,위진일보연구관상동맥미혈관조새적병리생리의의전정기출.지점、재료화간예:실험재복건의과대학부속협화의원서류동물실험실완성.실험선취24지SPF급웅성성년SD대서,안수궤수자표법장대서분위실험조화대조조,매조각12지.겸협승주동맥후자주동맥근부주입월계산납1.0 mg/kg,1.5 h후취표본.주요관찰지표:주입월계산납후대서관상동맥미혈관내피손해급미혈전형성정황;조기결양심기정황.결과:①주입월계산납1.5h후관상동맥미혈전조소유대서균출현미동맥혈관내피손상급미동맥혈전형성,대조조무차정황(P＜0.01);관상동맥미혈전조미동맥내피손상솔위(10.17±2.33)%,혈전형성솔위(9.42±2.02)%.②Nagar-Olsen염색현시심기결혈구역점소관찰심기횡절면면적적(7.52±2.26)%,대조조무심기결혈(P＜0.01).결론:월계산납가은정지유발관상동맥미혈전형성,차모형가작위관상동맥미혈전급미전새연구적평태.
BACKGROUND:Coronary microcirculation occlusion is very common in acute coronary syndrome(ACS), more importantly it leads to adverse prognosis.DESIGN:Single-blinded randomized controlled trial.OBJECTIVE:To develop a new model of microthrombosis in the coronary microcirculation for further study of pathophysiology of coronary microvascular occlusion.SETTING, PARTICIPANTS and INTERVENTIONS:The experiment was finished in the Laboratory of Union Hospital, Fujian Medical University.Twenty-four adult male rats(SPF grade) were randomly divided into experimental and control group by random number table, each consisted of 12.In the experimental group, 1.0 mg/kg of sodium laurate was injected into aorta during clamping the ascending aorta.The Samples were harvested 1.5 hours after injection.MAIN OUTCOME MEASURES:To observe endothelial damage, microthrombus in the coronary arterioles and myocardial ischemia after injection of sodium laurate.endothelial damage [(10.17±2.33)% of the coronary arterioles] and platelet-fibrin microthrombi [ (9.42 ± 2.02)% of the coronary arterioles]was induced in the coronary microcirculation 1.5 hours after injection of sodium laurate in all animals of the experimental group, without any changes in all control animals( P ＜ 0.01 ); Nagar-Olsen' s staining showed that ischemic myocardium was (7.52 ± 2.26)% of the total area of myocardial sections, without myocardial ischemic alteration in the control group ( P ＜0.01).CONCLUSION:Sodium laurate can induce coronary microthrombosis steadily, and this model can be used as a platform for the further study of coronary microthrombosis and microembolism.