CAJ | 학술논문

目的:已有一些临床试验和基础研究显示灯盏细辛(Gerigeron Breviscapus(vant)Hand-Maz2 EBHM),对青光眼患者及动物模型有神经保护的作用,本研究探讨灯盏细辛是否对NMDA导致的大鼠RGCL神经元兴奋毒性有保护作用.方法:60只健康SD大鼠随机分为4组,其中6只为正常对照组(A组),其余54只随机分为3组,分别为B组(EBHM组),C组(生理盐水+NMDA组),D组(EBHM+NMDA组),每组各18只大鼠.C、D两组大鼠右眼玻璃体内注射NMDA 10nmol/2μL制成视网膜损伤模型,左眼玻璃体内注射相同剂量PBS液作为自身对照.B组及D组均在NMDA注射前7d起按150mg/(kg·d)日剂量予60g/LEBHM腹腔内注射,C组予生理盐水0.5mL腹腔内注射.在NMDA处理后4,7,14d处死动物剥取视网膜作全层铺片行RGCL神经元计数分析.结果:正常对照组双眼RGCL神经元计数比较差异无显著性意义(P=0.200).NMDA干预后4,7,14d EBHM组RGCL神经元计数,双眼与正常对照组比较差异无显著性意义(P＞0.05).生理盐水+NMDA及EBHM+NMDA组实验眼在以上各时段RGCL神经元计数与正常组比较差异均有非常显著性意义(P＜0.001),左眼与正常对照组比较差异无显著性意义(P＞0.05).实验眼14d时RGCL神经元计数EBHM+NMDA组高于生理盐水+NMDA组,二者比较差异有显著性(P=0.044),但仍低于正常对照组(P＜0.05).结论:单独使用EBHM对正常大鼠RGCL神经元计数无影响,EBHM可对NMDA所致大鼠RGCL神经毒性提供部分保护作用.
목적:이유일사림상시험화기출연구현시등잔세신(Gerigeron Breviscapus(vant)Hand-Maz2 EBHM),대청광안환자급동물모형유신경보호적작용,본연구탐토등잔세신시부대NMDA도치적대서RGCL신경원흥강독성유보호작용.방법:60지건강SD대서수궤분위4조,기중6지위정상대조조(A조),기여54지수궤분위3조,분별위B조(EBHM조),C조(생리염수+NMDA조),D조(EBHM+NMDA조),매조각18지대서.C、D량조대서우안파리체내주사NMDA 10nmol/2μL제성시망막손상모형,좌안파리체내주사상동제량PBS액작위자신대조.B조급D조균재NMDA주사전7d기안150mg/(kg·d)일제량여60g/LEBHM복강내주사,C조여생리염수0.5mL복강내주사.재NMDA처리후4,7,14d처사동물박취시망막작전층포편행RGCL신경원계수분석.결과:정상대조조쌍안RGCL신경원계수비교차이무현저성의의(P=0.200).NMDA간예후4,7,14d EBHM조RGCL신경원계수,쌍안여정상대조조비교차이무현저성의의(P＞0.05).생리염수+NMDA급EBHM+NMDA조실험안재이상각시단RGCL신경원계수여정상조비교차이균유비상현저성의의(P＜0.001),좌안여정상대조조비교차이무현저성의의(P＞0.05).실험안14d시RGCL신경원계수EBHM+NMDA조고우생리염수+NMDA조,이자비교차이유현저성(P=0.044),단잉저우정상대조조(P＜0.05).결론:단독사용EBHM대정상대서RGCL신경원계수무영향,EBHM가대NMDA소치대서RGCL신경독성제공부분보호작용.
·AIM: To investigate whether Erigeron Breviscapus (vant) Hand-Mazz (EBHM) EBHM has neuroprotective effect against N-methyl-D-aspartate (NMDA)-induced neuron death in retinal ganglion cell layer (RGCL).· METHODS: 60 healthy SD rats were randomly divided into four groups. 6 animals were normal control group (group A). The others were divided as group B (EBHM group), group C (normal saline+NMDA group) and group D (EBHM + NMDA group). Each group had 18 rats.10nmol NMDA was intravitreally injected to induce partial damage of the neurons in RGCL in the right eyes of Groups C and D. Same volume PBS was intravitreally injected into the left eyes as self-control. Groups B and D were pre-treated intraperitoneally with 6g/L EBHM solution at a dose of 150mg/kg body weight/day seven days before and after NMDA treatment. Group C were administrated intraperitoneally with 9g/L normal saline at the same time of EBHM injection. Rats were sacrificed at 4,7,14d after NMDA treatment. Flat whole retinas were stained with 5g/L cresyl violet and neuron counting in RGCL from both eyes were observed. Each subgroup had 6 rats.· RESULTS: There was no significant difference of neuron counting in RGCL between the right eye and the left eye in group A (P=0.200). There was no significant difference between normal control group and EBHM group either in the right eyes or in the left eyes at 4, 7 and 14 d respectively after intravitreal injection of 10nmol NMDA in group C and group D. (P=0.636, P=0.193). Neuron counting of RGCL in group C and D was significantly decreased in the NMDA-treated eyes at 4, 7 and 14d after intravitreal injection (P＜0.001). There was no significant difference between self-control eyes group and normai control group(P＞0.05). However, neuron counting was significantly higher in the EBHM+NMDA group than normal saline +NMDA group at 14days after intravitreal injection (P=0.044), but was lowered than normal control group (P＜0.05).· CONCLUSION: EBHM has no effect on neuron counting of RGCL when administered alone in normal rats.The results indicates that EBHM plays a partial protective role in NMDA-induced neuron loss in RGCL in the rats.