CAJ | 학술논문

背景:周期素依赖性蛋白激酶5是周期素依赖性蛋白激酶家族的成员之一,脑发育过程中周期素依赖性蛋白激酶5mRNA及其蛋白在脑内的表达及分布状况与神经退行性变思维认知学的关系一直被研究者关注.目的:探讨大脑发育过程中的周期素依赖性蛋白激酶5对神经系统的发生和退行性变的影响.设计:单因素方差分析实验.单位:解放军第一军医大学组织学与胚胎学教研室和神经生物学教研室.材料:实验在解放军第三军医大学组织学与胚胎学实验室完成.Wistar大鼠胚胎期(E8～E21)、新生期(P0～P15)、幼年期(P16～2个月)、成年期(＞ 2个月)及老年期(＞8个月后)5个阶段25只大鼠,每组5只.方法:采用胚胎期至老年期大鼠脑片行原位杂交组织化学和免疫细胞化学染色.主要观察指标:不同脑区内周期素依赖性蛋白激酶5mRNA及其蛋白的阳性细胞分布及表达状况.结果:25只大鼠全部进入结果分析.①周期素依赖性蛋白激酶5mRNA在大鼠E14～P350整个发育过程中均有表达,成年后趋于稳定;周期素依赖性蛋白激酶5mRNA主要定位于神经元,阳性区域主要分布于大脑皮质、海马、丘脑、下丘脑、小脑及部分神经核团.②出生后周期素依赖性蛋白激酶5表达较强,胚胎及老年鼠表达较弱;阳性区域集中在室周区、海马、小脑及部分神经核团内;老年鼠仅在海马、小脑蒲肯野细胞层内表达.结论:脑内周期素依赖性蛋白激酶5的作用贯穿了整个神经发育的各个时期,在新生期、幼年期有较为显著的表达,成年后表达下降,尤以老年期显著.老年大鼠海马内周期素依赖性蛋白激酶5的表达下调可能与老年性学习记忆减退的发生密切相关.
배경:주기소의뢰성단백격매5시주기소의뢰성단백격매가족적성원지일,뇌발육과정중주기소의뢰성단백격매5mRNA급기단백재뇌내적표체급분포상황여신경퇴행성변사유인지학적관계일직피연구자관주.목적:탐토대뇌발육과정중적주기소의뢰성단백격매5대신경계통적발생화퇴행성변적영향.설계:단인소방차분석실험.단위:해방군제일군의대학조직학여배태학교연실화신경생물학교연실.재료:실험재해방군제삼군의대학조직학여배태학실험실완성.Wistar대서배태기(E8～E21)、신생기(P0～P15)、유년기(P16～2개월)、성년기(＞ 2개월)급노년기(＞8개월후)5개계단25지대서,매조5지.방법:채용배태기지노년기대서뇌편행원위잡교조직화학화면역세포화학염색.주요관찰지표:불동뇌구내주기소의뢰성단백격매5mRNA급기단백적양성세포분포급표체상황.결과:25지대서전부진입결과분석.①주기소의뢰성단백격매5mRNA재대서E14～P350정개발육과정중균유표체,성년후추우은정;주기소의뢰성단백격매5mRNA주요정위우신경원,양성구역주요분포우대뇌피질、해마、구뇌、하구뇌、소뇌급부분신경핵단.②출생후주기소의뢰성단백격매5표체교강,배태급노년서표체교약;양성구역집중재실주구、해마、소뇌급부분신경핵단내;노년서부재해마、소뇌포긍야세포층내표체.결론:뇌내주기소의뢰성단백격매5적작용관천료정개신경발육적각개시기,재신생기、유년기유교위현저적표체,성년후표체하강,우이노년기현저.노년대서해마내주기소의뢰성단백격매5적표체하조가능여노년성학습기억감퇴적발생밀절상관.
BACKGROUND: Cyclin-dependent kinase-5 (CDK-5) is one of the members in cyclin-dependent protein kinase family. The attention has being drawn by researchers on the relationship between the expression and distribution of CDK-5 mRNA and its protein in the brain during brain development and neural degeneration in thought-cognition.OBJECTIVE: To probe into the influence of CDK-5 on neurogeny and neural degeneration during cerebral development.DESIGN: Single factor analysis of variance.SETTING: Histological and Embryological Department and Neurobiological Department in Third Military Medical University of Chinese PLA.MATERIALS: The experiment was performed in Histological and Embryological Department and Neurobiological Department in Third Military Medical University of Chinese PLA. Twenty-five Wistar rats of 5 phases were employed, named embryonicphase (E8-E21), neonatal phase (P0-P15),childhood (P16-2 months), grown-up phase (＞ 2 months) and senile phase (＞ 8 months), 5 rats in each group.METHODS: In situ hybridization histochemistry (ISH) and immunohistochemistry (IHC) staining was adopted in brain sections from embryonic phase to senile phase.MAIN OUTCOME MEASURFS: Distribution and expression of positive cells of CDK-5 mRNA and protein in various brain areas.RESULTS: Twenty-five rats entered result analysis for all. ① The expression of CDK-5 mRNA presented in entire development from E14 to P350and was in tendency of stability after growth-up. CDK-5 mRNA localized mainly in neurons and positive regions distributed mainly in cerebral cortex, hippocampus, thalamus, hypothalamus, cerebellum and a part of nerve nuclei. ② The expression of CDK-5 was strong after birth and it was weaker in embryonic and senile rats. Positive regions concentrated mainly in peripheral ventricle, hippocampus, cerebellum and a part of nerve nuclei.The expression only presented in hippocampus and Purkinje cellular layer of cerebellum in senile rats.CONCLUSION: CDK-5 in brain runs through entire phases of neural development, it expresses more significantly in neonatal phase and childhood and declines after growth-up, especially in senile phase. The declined expression of CDK-5 in hippocampus of senile rats is closely associated with decline of learning and memory in senility probably.