中南大学学报(医学版)
中南大學學報(醫學版)
중남대학학보(의학판)
JOURNAL OF CENTRAL SOUTH UNIVERSITY（MEDICAL SCIENCES）
2008年
5期
425-431
,共7页
袁运长%夏振坤%章庆春%尹邦良
袁運長%夏振坤%章慶春%尹邦良
원운장%하진곤%장경춘%윤방량
急性排斥反应%结缔组织生长因子%纤维化%心脏移植
急性排斥反應%結締組織生長因子%纖維化%心髒移植
급성배척반응%결체조직생장인자%섬유화%심장이식
acute rejection%connective transforming growth factor%fibrosis%heart transplantation
目的:检测结缔组织生长因子(connective tissue growth factor,CTGF)在大鼠移植心脏急性排斥期(acute rejection,AR)的表达,并探讨其与移植心脏心肌纤维化的关系.方法:取Wister大鼠16只作为供体,取SD大鼠16只作为受体,进行腹部异位心脏移植,采用10 mg/(kg·d) 环孢霉素A,40 mg/(kg·d)骁悉和3 mg/(kg·d)甲泼尼龙三联免疫抑制疗法,2周后取其中10例移植心脏标本作为实验组,10例健康Wister大鼠心脏标本作为对照组(normal heart,NH).采用HE及van Gieson染色观察心肌病理形态、心脏血管狭窄程度;并进行纤维化的半定量评分.采用免疫组化半定量方法检测CTGF在心脏组织中的表达,并分析其与心肌纤维化的相关性.结果:移植心脏血管壁及心肌间质组织内有大量粒细胞浸润,呈典型的急性排斥反应期改变;移植心脏血管内膜及大量纤维组织增生,与正常对照组相比,差异有统计学意义(P<0.05);CTGF在移植心脏组织中有明显表达,且主要表达于血管壁及心肌损伤部位,而正常心脏无表达,CTGF灰度值为 (AR vs NH: 103.52±6.42 vs. 182.61±8.72,P<0.05).CTGF灰度值与移植心脏纤维化显著负相关(r=-0.734,P<0.01).结论:CTGF在急性移植心脏组织中有明显表达且可能与移植心脏心肌纤维化相关.
目的:檢測結締組織生長因子(connective tissue growth factor,CTGF)在大鼠移植心髒急性排斥期(acute rejection,AR)的錶達,併探討其與移植心髒心肌纖維化的關繫.方法:取Wister大鼠16隻作為供體,取SD大鼠16隻作為受體,進行腹部異位心髒移植,採用10 mg/(kg·d) 環孢黴素A,40 mg/(kg·d)驍悉和3 mg/(kg·d)甲潑尼龍三聯免疫抑製療法,2週後取其中10例移植心髒標本作為實驗組,10例健康Wister大鼠心髒標本作為對照組(normal heart,NH).採用HE及van Gieson染色觀察心肌病理形態、心髒血管狹窄程度;併進行纖維化的半定量評分.採用免疫組化半定量方法檢測CTGF在心髒組織中的錶達,併分析其與心肌纖維化的相關性.結果:移植心髒血管壁及心肌間質組織內有大量粒細胞浸潤,呈典型的急性排斥反應期改變;移植心髒血管內膜及大量纖維組織增生,與正常對照組相比,差異有統計學意義(P<0.05);CTGF在移植心髒組織中有明顯錶達,且主要錶達于血管壁及心肌損傷部位,而正常心髒無錶達,CTGF灰度值為 (AR vs NH: 103.52±6.42 vs. 182.61±8.72,P<0.05).CTGF灰度值與移植心髒纖維化顯著負相關(r=-0.734,P<0.01).結論:CTGF在急性移植心髒組織中有明顯錶達且可能與移植心髒心肌纖維化相關.
목적:검측결체조직생장인자(connective tissue growth factor,CTGF)재대서이식심장급성배척기(acute rejection,AR)적표체,병탐토기여이식심장심기섬유화적관계.방법:취Wister대서16지작위공체,취SD대서16지작위수체,진행복부이위심장이식,채용10 mg/(kg·d) 배포매소A,40 mg/(kg·d)효실화3 mg/(kg·d)갑발니룡삼련면역억제요법,2주후취기중10례이식심장표본작위실험조,10례건강Wister대서심장표본작위대조조(normal heart,NH).채용HE급van Gieson염색관찰심기병리형태、심장혈관협착정도;병진행섬유화적반정량평분.채용면역조화반정량방법검측CTGF재심장조직중적표체,병분석기여심기섬유화적상관성.결과:이식심장혈관벽급심기간질조직내유대량립세포침윤,정전형적급성배척반응기개변;이식심장혈관내막급대량섬유조직증생,여정상대조조상비,차이유통계학의의(P<0.05);CTGF재이식심장조직중유명현표체,차주요표체우혈관벽급심기손상부위,이정상심장무표체,CTGF회도치위 (AR vs NH: 103.52±6.42 vs. 182.61±8.72,P<0.05).CTGF회도치여이식심장섬유화현저부상관(r=-0.734,P<0.01).결론:CTGF재급성이식심장조직중유명현표체차가능여이식심장심기섬유화상관.
Objective To detect the expression of connective tissue growth factor (CTGF) in acute heart allograft rejection in rats and to investigate the relationship between CTGF expression and cardiac allograft fibrosis. Methods Sixteen Wister rats served as donors and another 16 Sprague-Dawely (SD) rats served as recipients. Intra-abdominal heterotopic heart transplantation was performed. All rats received 10 mg/(kg·d) cyclosporine,40 mg/(kg·d)CellCept, and 3 mg/(kg·d)methylprednisolone immunosuppression after the surgery. Ten allografts were harvested 2 weeks postoperation while 10 normal Wister rats served as controls. The paraffin sections of harvested heart specimens were stained with hematoxylin and eosin (HE),and van Gieson(VG) for the examination of morphological changes to observe the lumen loss of myocardial coronary arteries and myocardial fibrosis. The expression of CTGF was studied by immunnohistochemical method and was measured semi quantitatively. The correlation between the CTGF expression and allograft fibrosis was studied. Results The allografts showed a typical symbol of acute rejection with excessive granulocyte infiltration around the vessel wall and myocardial interstice. There were also intimal proliferation and obvious fibrosis in the acute group and the differences between the acute and control group were significant (P<0.05). The expression of CTGF protein was mainly located around the vascular and myocardial lesions in the acute group while the control group showed no CTGF expression. The gray scale value of CTGF was (AR vs NH: 103.52±6.42 vs. 182.61±8.72,P<0.05). Strong negative correlations were found between the gray scale value and fibrosis formation(r=-0.734,P<0.01). Conclusion CTGF was overexpressed in acute allograft rejection rat hearts and might be involved in the pathogenesis of transplanted heart fibrosis.
