CAJ | 학술논문

目的:在裸鼠体内转入HBx基因的QSG7701细胞株形成移植瘤,并探讨裸鼠成瘤的可能机制.方法:利用已成功构建的高表达HBx基因的pCMVX/QSG7701细胞株作为实验组,以pRcCMV2/QSG7701细胞株和QSG7701细胞株为对照组接种于裸鼠皮下,观察裸鼠能否成瘤.HE染色研究移植瘤的组织形态学特征,以RT-PCR法检测移植瘤组织、pCMVX/QSG7701细胞、pRcCMV2/QSG7701细胞及QSG7701细胞的p53基因和c-Myc基因表达情况.结果:接种pCMVX/QSG7701细胞株的裸鼠在第2周开始出现移植瘤生长,周围器官和组织未发现转移灶.对照组至接种后第35天无移植瘤生长.HE染色证实移植瘤为肝细胞癌组织.移植瘤和pCMVX/QSG7701细胞中突变型p53和c-Myc mRNA的表达量较pRcCMV2/QSG7701细胞和QSG7701细胞明显增高(P＜0.01).结论:HBx基因表达可以直接导致肝细胞癌变;HBx能上调移植瘤和pMVX/QSG7701细胞中突变型p53和c-Myc的表达,并可能通过这一机制导致肝细胞癌变.
목적:재라서체내전입HBx기인적QSG7701세포주형성이식류,병탐토라서성류적가능궤제.방법:이용이성공구건적고표체HBx기인적pCMVX/QSG7701세포주작위실험조,이pRcCMV2/QSG7701세포주화QSG7701세포주위대조조접충우라서피하,관찰라서능부성류.HE염색연구이식류적조직형태학특정,이RT-PCR법검측이식류조직、pCMVX/QSG7701세포、pRcCMV2/QSG7701세포급QSG7701세포적p53기인화c-Myc기인표체정황.결과:접충pCMVX/QSG7701세포주적라서재제2주개시출현이식류생장,주위기관화조직미발현전이조.대조조지접충후제35천무이식류생장.HE염색증실이식류위간세포암조직.이식류화pCMVX/QSG7701세포중돌변형p53화c-Myc mRNA적표체량교pRcCMV2/QSG7701세포화QSG7701세포명현증고(P＜0.01).결론:HBx기인표체가이직접도치간세포암변;HBx능상조이식류화pMVX/QSG7701세포중돌변형p53화c-Myc적표체,병가능통과저일궤제도치간세포암변.
Objective To determine whether HBx gene can directly induce hepatocellular carcinoma in vivo, and to explore the mechanism of transplantation tumor in nude mice.Methods pCMVX/QSG7701 cell lines were vaccinated into subcutaneous tissue of nude mice. pRcCMV2/QSG7701 and QSG7701 cell lines were used as controls. The sections of transplantation tumor were observed microscopically by HE staining. RT-PCR was used to detect the expression of mutant p53 and c-Myc mRNA in transplant tumor and an other 3 cell lines. Results The transplant tumor occurred within the subcutaneous tissue of the nude mice inoculated with pCMVX/QSG7701 cell lines at 2nd week after the vaccination. No metastatic tumor was found in other organs. Transplant tumor was not formed in all the controls. HE staining confirmed that the transplant tumor was hepatocellular carcinoma. The mutant p53 mRNA and c-Myc mRNA expression level of transplant tumor and pCMVX/QSG7701 cells was significantly higher than that of pRcCMV2/QSG7701 and QSG7701 cells, respectively (P＜0.01).Conclusion HBx gene can up-regulate the expression of mutant p53 and c-Myc genes, and directly induce hepatocellular carcinoma in vivo.