中药新药与临床药理
中藥新藥與臨床藥理
중약신약여림상약리
TRADITIONAL CHINESE DRUG RESEARCH&CLINICAL PHARMACOLOGY
2009年
4期
356-358
,共3页
叶会呈%叶其馨%王带媚%曾丽云
葉會呈%葉其馨%王帶媚%曾麗雲
협회정%협기형%왕대미%증려운
血府逐瘀汤%阿魏酸%含量测定%HPLC
血府逐瘀湯%阿魏痠%含量測定%HPLC
혈부축어탕%아위산%함량측정%HPLC
Xuefu Zhuyu Decoction%Ferulic acid%Determination%HPLC
目的 建立血府逐瘀汤中阿魏酸含量的测定方法.方法 采用高效液相色谱法,色谱柱:KromasilCn(250mm×4.6 mm,5 μm);流动相:乙腈-0.085%磷酸(17:83);流速:1.0 mL/min;检测波长:316 nm;柱温:35℃.结果 阿魏酸在0.0252μg～0.504μg范围内线性关系良好,r=0.9999,平均回收率为100.15%,RSD=1.69%.结论 该法简单,快捷,适用于血府逐瘀汤中阿魏酸的含量测定.
目的 建立血府逐瘀湯中阿魏痠含量的測定方法.方法 採用高效液相色譜法,色譜柱:KromasilCn(250mm×4.6 mm,5 μm);流動相:乙腈-0.085%燐痠(17:83);流速:1.0 mL/min;檢測波長:316 nm;柱溫:35℃.結果 阿魏痠在0.0252μg～0.504μg範圍內線性關繫良好,r=0.9999,平均迴收率為100.15%,RSD=1.69%.結論 該法簡單,快捷,適用于血府逐瘀湯中阿魏痠的含量測定.
목적 건립혈부축어탕중아위산함량적측정방법.방법 채용고효액상색보법,색보주:KromasilCn(250mm×4.6 mm,5 μm);류동상:을정-0.085%린산(17:83);류속:1.0 mL/min;검측파장:316 nm;주온:35℃.결과 아위산재0.0252μg～0.504μg범위내선성관계량호,r=0.9999,평균회수솔위100.15%,RSD=1.69%.결론 해법간단,쾌첩,괄용우혈부축어탕중아위산적함량측정.
Objective To establish a HPLC method for the determination of ferulic acid in Xuefu Zhuyu Decoction. Methods A Kromasil C18(250 mm ×4. 6 mm, 5 μm) column was adopted. The mobile phase consisted of acetonitrile-0. 085 % phos-phoric acid(17 : 83)with the flow rate of 1.0 mL/min. The column temperature was at 35 ℃ and detection wavelength was set at 316 nm. Results A good linearity of ferulic acid was in the range of 0. 0252μg to 0. 504μg and r=0. 999 9. The average recovery was 100. 15 % and RSD=1.69 %. Conclusion The method is simple and rapid, and it is suitable for the determi-nation of ferulic acid in Xuefu Zhuyu Decoction.
