CAJ | 학술논문

背景与目的:研究氯化镉对睾丸指数及睾丸细胞线粒体ATPase6、D-Loop基因突变的影响. 材料与方法:取40只体重(19.5±2.5)g的雄性ICR小鼠,随机分为4组,每组10只,分别以1、5、10 μmol/kg氯化镉腹腔注射,隔天注射1次,共10次,同时设阴性对照组(生理盐水).第21天取小鼠双侧睾丸称重并计算睾丸指数;提取睾丸组织基因组DNA,PCR扩增线粒体ATPase6、D-Loop基因,纯化后测序分析基因突变.结果:10 μmol/kg氯化镉组睾丸指数显著低于对照组(P<0.01),未检测到小鼠睾丸细胞线粒体ATPase6、D-Loop基因的突变.结论:10 μmol/kg浓度的氯化镉可致小鼠睾丸指数降低,氯化镉短期处理(21 d内)未能引起小鼠睾丸线粒体ATPase6、D-oop基因突变.睾丸指数与细胞线粒体ATPase6、D_oop基因突变可能不存在相关性.
배경여목적:연구록화력대고환지수급고환세포선립체ATPase6、D-Loop기인돌변적영향. 재료여방법:취40지체중(19.5±2.5)g적웅성ICR소서,수궤분위4조,매조10지,분별이1、5、10 μmol/kg록화력복강주사,격천주사1차,공10차,동시설음성대조조(생리염수).제21천취소서쌍측고환칭중병계산고환지수;제취고환조직기인조DNA,PCR확증선립체ATPase6、D-Loop기인,순화후측서분석기인돌변.결과:10 μmol/kg록화력조고환지수현저저우대조조(P<0.01),미검측도소서고환세포선립체ATPase6、D-Loop기인적돌변.결론:10 μmol/kg농도적록화력가치소서고환지수강저,록화력단기처리(21 d내)미능인기소서고환선립체ATPase6、D-oop기인돌변.고환지수여세포선립체ATPase6、D_oop기인돌변가능불존재상관성.
BACKGROUND AND AIM: To study testis index and mtATPase6 and D-Loop point mutations of mice testicle after exposure to cadmium. MATERIALS AND METHODS: 40 male mice were divided into 4 groups with 10 animals per group (weight: 19.5 2.5 g).3 groups were treated with intraperitoneal cadmium chloride of 1,5,10 μmolAg,once every 2 days, 10 times in total,and one normal saline control group. After exposure to cadmium chloride for 20 days, we determined the testis index. The mtATPase6 and D-Loop genes were amplified by PCR from the genomic DNA of mice testicle tissue, with their mutant sites sequenced. RESULTS: The testis index of mice in the 10 μmol/kg cadmium chloride group were lower than that of the control group (P < 0.01) . No mtATPase6 or D-Loop mutation was found in the murine spermatogenic cells. CONCLUSION: Mice testis index was lower by 10 μmol/ kg cadmium chloride. But cadmium chloride couldn' t induce mtATPase6 and D-Loop mutations in the mice testicle. Testis index and mtATPase6 and D-Loop point mutations did not seem to be related.