CAJ | 학술논문

背景:间质干细胞或间质祖细胞的非造血细胞可在体内或体外分化为骨、软骨、肌肉、肌腱、脂肪及骨髓基质,这使得它们成为组织工程领域内非常有价值的种子细胞来源.目的:以骨髓间充质干细胞为种子细胞,以Ⅰ型胶原-聚羟基乙酸为支架构建组织工程化肌腱,观察其修复跟腱缺损的效果.设计,时间及地点:随机对照动物实验,于2003年在中南大学湘雅医学院进行.材料:1.5～2.5 kg健康成年家兔由中南大学湘雅医学院动物学部提供,雌雄不限.聚羟基乙酸由威高集团康利达医用制品有限公司提供.SD大鼠由中南大学实验动物学部提供.方法:提取家兔骨髓,通过离心和贴壁法培养获取骨髓间充质干细胞,将骨髓间充质干细胞进行分离、扩增.抽取SD大鼠尾腱,制备鼠尾Ⅰ型胶原溶液,并与聚羟基乙酸缝线混合培养构建胶原-聚羟基乙酸支架.将未经体外诱导的骨髓间充质干细胞接种于胶原-聚羟基乙酸支架中构建组织工程化兔肌腱模型,以不加入细胞的为对照.切开30只家兔踝部皮肤,分离出兔跟腱,造成3cm长缺损,实验组15只以自体骨髓间充质干细胞预制的组织工程化肌腱移植于缺损处,对照组15只以Ⅰ型胶原-聚羟基乙酸支架修复跟腱缺损.主要观察指标:组织工程化肌腱修复兔跟腱缺损的效果.结果:含自体骨髓间充质干细胞的Ⅰ型胶原一聚羟基乙酸支架及不含自体骨髓间充质干细胞的Ⅰ型胶原-聚羟基乙酸支架回植于体内时大体观察呈条形凝胶状.回植于体内4周后实验组和对照组均可见移植处形成条索状组织,聚羟基乙酸缝线已降解.8周时观察可见实验组组织工程化肌腱与受体肌腱组织完好连接,呈条索状.与周围其他组织无粘连,为白色、有光泽的致密腱样组织,对照组所形成的组织较实验组细小、与周围组织有粘连.结论:以骨髓间充质干细胞为种子细胞,以Ⅰ型胶原-聚羟基乙酸为支架构建的组织工程化肌腱可明显促进肌腱损伤的修复. 揹景:間質榦細胞或間質祖細胞的非造血細胞可在體內或體外分化為骨、軟骨、肌肉、肌腱、脂肪及骨髓基質,這使得它們成為組織工程領域內非常有價值的種子細胞來源.目的:以骨髓間充質榦細胞為種子細胞,以Ⅰ型膠原-聚羥基乙痠為支架構建組織工程化肌腱,觀察其脩複跟腱缺損的效果.設計,時間及地點:隨機對照動物實驗,于2003年在中南大學湘雅醫學院進行.材料:1.5～2.5 kg健康成年傢兔由中南大學湘雅醫學院動物學部提供,雌雄不限.聚羥基乙痠由威高集糰康利達醫用製品有限公司提供.SD大鼠由中南大學實驗動物學部提供.方法:提取傢兔骨髓,通過離心和貼壁法培養穫取骨髓間充質榦細胞,將骨髓間充質榦細胞進行分離、擴增.抽取SD大鼠尾腱,製備鼠尾Ⅰ型膠原溶液,併與聚羥基乙痠縫線混閤培養構建膠原-聚羥基乙痠支架.將未經體外誘導的骨髓間充質榦細胞接種于膠原-聚羥基乙痠支架中構建組織工程化兔肌腱模型,以不加入細胞的為對照.切開30隻傢兔踝部皮膚,分離齣兔跟腱,造成3cm長缺損,實驗組15隻以自體骨髓間充質榦細胞預製的組織工程化肌腱移植于缺損處,對照組15隻以Ⅰ型膠原-聚羥基乙痠支架脩複跟腱缺損.主要觀察指標:組織工程化肌腱脩複兔跟腱缺損的效果.結果:含自體骨髓間充質榦細胞的Ⅰ型膠原一聚羥基乙痠支架及不含自體骨髓間充質榦細胞的Ⅰ型膠原-聚羥基乙痠支架迴植于體內時大體觀察呈條形凝膠狀.迴植于體內4週後實驗組和對照組均可見移植處形成條索狀組織,聚羥基乙痠縫線已降解.8週時觀察可見實驗組組織工程化肌腱與受體肌腱組織完好連接,呈條索狀.與週圍其他組織無粘連,為白色、有光澤的緻密腱樣組織,對照組所形成的組織較實驗組細小、與週圍組織有粘連.結論:以骨髓間充質榦細胞為種子細胞,以Ⅰ型膠原-聚羥基乙痠為支架構建的組織工程化肌腱可明顯促進肌腱損傷的脩複.
배경:간질간세포혹간질조세포적비조혈세포가재체내혹체외분화위골、연골、기육、기건、지방급골수기질,저사득타문성위조직공정영역내비상유개치적충자세포래원.목적:이골수간충질간세포위충자세포,이Ⅰ형효원-취간기을산위지가구건조직공정화기건,관찰기수복근건결손적효과.설계,시간급지점:수궤대조동물실험,우2003년재중남대학상아의학원진행.재료:1.5～2.5 kg건강성년가토유중남대학상아의학원동물학부제공,자웅불한.취간기을산유위고집단강리체의용제품유한공사제공.SD대서유중남대학실험동물학부제공.방법:제취가토골수,통과리심화첩벽법배양획취골수간충질간세포,장골수간충질간세포진행분리、확증.추취SD대서미건,제비서미Ⅰ형효원용액,병여취간기을산봉선혼합배양구건효원-취간기을산지가.장미경체외유도적골수간충질간세포접충우효원-취간기을산지가중구건조직공정화토기건모형,이불가입세포적위대조.절개30지가토과부피부,분리출토근건,조성3cm장결손,실험조15지이자체골수간충질간세포예제적조직공정화기건이식우결손처,대조조15지이Ⅰ형효원-취간기을산지가수복근건결손.주요관찰지표:조직공정화기건수복토근건결손적효과.결과:함자체골수간충질간세포적Ⅰ형효원일취간기을산지가급불함자체골수간충질간세포적Ⅰ형효원-취간기을산지가회식우체내시대체관찰정조형응효상.회식우체내4주후실험조화대조조균가견이식처형성조색상조직,취간기을산봉선이강해.8주시관찰가견실험조조직공정화기건여수체기건조직완호련접,정조색상.여주위기타조직무점련,위백색、유광택적치밀건양조직,대조조소형성적조직교실험조세소、여주위조직유점련.결론:이골수간충질간세포위충자세포,이Ⅰ형효원-취간기을산위지가구건적조직공정화기건가명현촉진기건손상적수복.
BACKGROUND:Non-hematopoietic cell of mesenchymal stem cells (MSCs) or mesenchymal progenitor cells can differentiate into bone,cartilage,muscle,tendon,fat and bone marrow matrix in vivo and in vitro,thus becoming very valuable seed cells source in the field of tissue engineering.OBJECTIVE:To construct the tissue engineered tendon withⅠcollagen-polyglycolic acid as a scaffold and bone marrow MSCs as seed cells,and to observe the effect of tissue engineered tendon on repairing achilles tendon defects. DESIGN,TIME AND SETTING:Randomized controlled animal experiment was performed in the Xiangya Medical College of Central South University in 2003.MATERIALS:Healthy adult rabbits,irrespective of genders,weighing 1.5-2.5 kg,were offered by Animal Center of Xiangya Medical College,Central South University. Polyglycolic acid were purchased from Shandong Weigao Group Kanglida Medical Products Co.,Ltd (KLD Medical).SD rats were sourced from Department of Experimental Animals,Central South University.METHODS:Rabbit bone marrow was extracted to harvest MSCs with centrrfugation and adherence method,then bone marrow MSCs were isolated and amplified. Tail tendon was extracted from SD rats and prepared into type Ⅰ collagen solution,which was mixed and suture cultured with polyglycolic acid to construct collagen-polyglycolic acid scaffold. Other bone marrow MSCs,which were not induced in vitro,were incubated on collagen-polyglycolic acid scaffold to construct tissue engineered rabbit tendon models,with those without cells serving as controls. Thirty rabbit angle skins were cut open to separate tendon and produce a 3-cm defect. Fifteen rats in the experiment group was repaired with tissue engineered tendon,which was previously prepared with autologous bone marrow MSCs,while fifteen rats in the control group was given Ⅰ collagen-polyglycolic acid scaffold.MAIN OUTCOME MEASURES:Effect of tissue engineered tendon on repairing achilles tendon defects in rabbit.RESULTS:No matter whether contains autologous bone marrow MSCs,type I collagen-polyglycolic acid scaffold transplanted into animals exhibited column gel shape by general observation. At 4 weeks following transplantation,cordlike tissues were seen in the transplantation site,polyglycolic acid suture was degraded. At 8 weeks,the tissue engineered tendon tissues were cordlike shaped,white,lustrous and dense. They were well connected with receptor tendon tissues in the experiment group,without adhesion to peripheral tissues. In the control group,the tissues were slender and adhered to peripheral tissues. CONCLUSION:Using typeⅠ collagen-polyglycolic acid as a scaffold and bone marrow MSCs as seed cells,tissue engineered tendon can dramatically promote the repair of achilles tendon defects.