CAJ | 학술논문

Cre重组酶来源于噬菌体P1基因组中的一段DNA序列编码的蛋白,是一种无需辅助因子即可进行位点特异性重组的生物酶.它的发现为基因靶位操作创造了有利条件,由于其作用方式高效简单,Cre定位重组系统已在特定基因的删除、基因功能的鉴定、外源基因的整合、基因捕获及染色体工程等方面得到了有效的利用,文章就cre重组酶的结构、功能、突变分析和改造及Cre重组酶的应用潜力等方面的研究进行了综述.
Cre중조매래원우서균체P1기인조중적일단DNA서렬편마적단백,시일충무수보조인자즉가진행위점특이성중조적생물매.타적발현위기인파위조작창조료유리조건,유우기작용방식고효간단,Cre정위중조계통이재특정기인적산제、기인공능적감정、외원기인적정합、기인포획급염색체공정등방면득도료유효적이용,문장취cre중조매적결구、공능、돌변분석화개조급Cre중조매적응용잠력등방면적연구진행료종술.
The Cre recombinase,a DNA-encoded protein from bacteriophage P1 genome sequence,is a kind of enzyme of site-specific reorganization without supporting factor.Its found has created favorable conditions for the operation of the gene target.Because of its simplicity and high efficiency,Cre site-specific recombination system has been widely used in gene deletion and function identification,gene site-specific integration,gene trapping and chromosome engineering,it has been used as a useful tool for biology.In the article,it summarized the study of the structure,function,mutation analysis and modification,and potential applications of Cre recombinase.