时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
2期
404-405
,共2页
付煊赫%刘同祥%张宗申%王继峰
付煊赫%劉同祥%張宗申%王繼峰
부훤혁%류동상%장종신%왕계봉
鹰嘴豆%胰蛋白酶抑制剂%正交实验
鷹嘴豆%胰蛋白酶抑製劑%正交實驗
응취두%이단백매억제제%정교실험
Cicer arietinum L.%Trypsin inhibitor%orthogonal design
目的 研究鹰嘴豆中胰蛋白酶抑制剂的最佳提取条件.方法 以提取液对胰蛋白酶的抑制率为指标,采用正交实验法对提取温度(A)、溶剂用量(B)、pH值(C)、提取时间(D)4个因素进行优选,并对优选的提取方案进行验证.结果 在所选水平范围内,因素C和B对提取效果有显著的影响,因素D和A影响不显著.结论 该文属国内首次关于鹰嘴豆胰蛋白酶抑制剂成分的研究报告,其有效部位的最佳提取工艺为C_1B_3D_1A_3.
目的 研究鷹嘴豆中胰蛋白酶抑製劑的最佳提取條件.方法 以提取液對胰蛋白酶的抑製率為指標,採用正交實驗法對提取溫度(A)、溶劑用量(B)、pH值(C)、提取時間(D)4箇因素進行優選,併對優選的提取方案進行驗證.結果 在所選水平範圍內,因素C和B對提取效果有顯著的影響,因素D和A影響不顯著.結論 該文屬國內首次關于鷹嘴豆胰蛋白酶抑製劑成分的研究報告,其有效部位的最佳提取工藝為C_1B_3D_1A_3.
목적 연구응취두중이단백매억제제적최가제취조건.방법 이제취액대이단백매적억제솔위지표,채용정교실험법대제취온도(A)、용제용량(B)、pH치(C)、제취시간(D)4개인소진행우선,병대우선적제취방안진행험증.결과 재소선수평범위내,인소C화B대제취효과유현저적영향,인소D화A영향불현저.결론 해문속국내수차관우응취두이단백매억제제성분적연구보고,기유효부위적최가제취공예위C_1B_3D_1A_3.
Objective To optimize the extraction procedure of trypsin inhibitor from Cicer arietinum L..Methods The inhibition rate of trypsin activity was used as screening parameter, and then optimize the four factors including temperature of extraction solution (A), volume of extraction solution (B), pH value of extraction solution(C) and time of extraction (D), thereafter, practise the optimal procedure. Results Factors C and B displayed the most significant effect on the extraction, and Factors D and A came the next.Conclusion For the first time, we documented the identification of TI and its extraction procedure from Cicer arietinum L., and the optimal extraction procedure was C_1B_3D_1A_3 when the inhibition ratio of trypsin activity was considered as preferential parameter.
