CAJ | 학술논문

目的:了解脐带血单个核细胞(CBMCs)及其CD4~+和CD8~+T细胞亚群中转录因子Elf-1的表达特点.方法:采用SYBR GreenⅠ荧光定量PCR和相对定量分析法检测12例脐带血CBMCs、CD4~+和CD8~+T细胞亚群中Elf-1基因的表达情况,以β_2微球蛋白基因(β_2M)作为内参照,10例健康成人作为对照,采用相对定量公式:2~(-△Ct)×100%,计算Elf-1 mRNA相对表达量.结果:脐带血和健康成人外周血单个核细胞(PBMC)、CD4~+和CD8~+T细胞均表达Elf-1,具体表达量呈现明显的个体差异性.Elf-1 mRNA相对表达量在CBMCs为18.55%±2.48%(其中最高为36.22%,最低为6.45%),在脐带血CD8~+T细胞为3.52%±0.45%(其中最高为6.75%,最低为1.71%),均明显高于健康成人PBMC(9.16%±1.92%)及其CD8~+ T细胞(2.02%±0.27%)(P<0.01,P<0.05),而脐带血CD4~+T细胞Elf-1 mRNA 相对表达量为3.83%±0.61%,与健康成人外周血CD4~+T细胞(2.73±0.52%)比较,无显著差异.结论:本研究率先报道脐带血T细胞亚群中Elf-1 mRNA表达特点,结果提示Elf-1基因在CBMCs及其CD8~+T细胞亚群中的高表达可能是T细胞的免疫学特点之一.
목적:료해제대혈단개핵세포(CBMCs)급기CD4~+화CD8~+T세포아군중전록인자Elf-1적표체특점.방법:채용SYBR GreenⅠ형광정량PCR화상대정량분석법검측12례제대혈CBMCs、CD4~+화CD8~+T세포아군중Elf-1기인적표체정황,이β_2미구단백기인(β_2M)작위내삼조,10례건강성인작위대조,채용상대정량공식:2~(-△Ct)×100%,계산Elf-1 mRNA상대표체량.결과:제대혈화건강성인외주혈단개핵세포(PBMC)、CD4~+화CD8~+T세포균표체Elf-1,구체표체량정현명현적개체차이성.Elf-1 mRNA상대표체량재CBMCs위18.55%±2.48%(기중최고위36.22%,최저위6.45%),재제대혈CD8~+T세포위3.52%±0.45%(기중최고위6.75%,최저위1.71%),균명현고우건강성인PBMC(9.16%±1.92%)급기CD8~+ T세포(2.02%±0.27%)(P<0.01,P<0.05),이제대혈CD4~+T세포Elf-1 mRNA 상대표체량위3.83%±0.61%,여건강성인외주혈CD4~+T세포(2.73±0.52%)비교,무현저차이.결론:본연구솔선보도제대혈T세포아군중Elf-1 mRNA표체특점,결과제시Elf-1기인재CBMCs급기CD8~+T세포아군중적고표체가능시T세포적면역학특점지일.
AIM: To investigate the expression level of transcriptional factor Elf-1 in mononuclear cells, CD4~+ and CD8~+ T cells from umbilical cord blood (UCB). METHODS: Real-time PCR with SYBR green I technique was used for detecting the Elf-1 expression in mononuclear cells, sorted CD4~+ and CD8~+ T cells from 12 cases of umbilical cord blood. The relative mRNA expression level of Elf-1 was analyzed by a formula of 2~(-△Ct)×100%. The expression level of β_2-microglobulin gene (β_2M) was used as an endogenous reference. The peripheral blood from 10 cases of healthy adults was served as control. RESULTS: Elf-1 mRNA expressed in all blood samples collected from both UCB and healthy adults. The expression level showed apparent diversity in different individuals. The relative mRNA expression of Elf-1 in both mononuclear cells (18.55%±2.48%) and CD8~+ T cells (3.52%±0.45%) from UCB were significantly higher than those from healthy adults (9.16%±1.92%, 2.02%±0.27%, respectively, P<0.01, P<0.05). CONCLUSION: The results of Elf-1 expression level from umbilical cord blood indicate that the over-expression of Elf-1 gene in mononuclear cells and CD8~+ T cells might be one of the features of T cell immune state in umbilical cord blood.