CAJ | 학술논문

将人类巨细胞病毒HCMV Towne株接种至人包皮成纤维细胞(HFF)中,收集病毒感染后的细胞,抽提病毒RNA,运用RT-PCR技术扩增基因的序列,对PCR产物作TA克隆,重组体进行测序和生物信息学分析.结果表明,HCMV的UL49区域存在一个新转录本,暂命名为UL49X.将所获得的序列与HCMV基因组比对分析表明,UL49X序列为68 783 bp到78 608 bp处的基因片段,并发现从69 194 bp到71 530 bp处有个典型的内含子.
장인류거세포병독HCMV Towne주접충지인포피성섬유세포(HFF)중,수집병독감염후적세포,추제병독RNA,운용RT-PCR기술확증기인적서렬,대PCR산물작TA극륭,중조체진행측서화생물신식학분석.결과표명,HCMV적UL49구역존재일개신전록본,잠명명위UL49X.장소획득적서렬여HCMV기인조비대분석표명,UL49X서렬위68 783 bp도78 608 bp처적기인편단,병발현종69 194 bp도71 530 bp처유개전형적내함자.
Incubated HCMV Towne to the well growing HFF cells;Collected the cells while the HFF cells appearing eytopathic effects;Extracted the HCMV RNA from the infected cells.Amplified the cDNA of the gene by RT-PCR.Finally,the production of RT-PCR were cloned into pMD18-T vectors and sequenced subsequently.And the sequence of UL49X was analyzed by bioinformatics.Our research show that, there is a new gene in the UL49 region of HCMV which we called it UL49X.The sequence of UL49X gene was from 68 783 bp to 78 608 bp,and we found that there was a intron between 69 194 bp and 71 530 bp.