中华细胞与干细胞杂志（电子版）
中華細胞與榦細胞雜誌（電子版）
중화세포여간세포잡지（전자판）
CHINESE JOURNAL OF CELL AND STEM CELL
2012年
1期
23-26
,共4页
白守民%薛卫平%刘宜敏%毕卓菲%梁碧玲
白守民%薛衛平%劉宜敏%畢卓菲%樑碧玲
백수민%설위평%류의민%필탁비%량벽령
神经干细胞%辐射损伤%超顺磁性氧化铁%脑损伤%磁共振成像
神經榦細胞%輻射損傷%超順磁性氧化鐵%腦損傷%磁共振成像
신경간세포%복사손상%초순자성양화철%뇌손상%자공진성상
Neural stem cell%Radiation injury%Superparamagnetic iron oxide%Braininjury%Magnetic resonance imaging
目的 探讨用超顺磁性氧化铁(SPIO)标记的神经干细胞(NSCs)在放射性脑损伤大鼠脑的磁共振(MR)显像及迁移.方法 从大鼠胎鼠脑组织中分离、培养NSCs.采用nestin抗原表达鉴定培养的NSCs.采用电子线半脑照射法制作大鼠放射损伤模型.用SPIO标记NSCs,根据大鼠脑立体定位图谱,将NSCs植入大鼠脑内.行MR检查,了解干细胞的成像情况及在放射后大鼠脑内的分布迁移.结果 分离、培养的细胞表达nestin.标记的NSCs可以在MR显像,成像可以持续1个月以上.移植术后可以发现干细胞向放射部位的迁移.结论 MR可以显示干细胞向放射损伤部位的定向迁移.
目的 探討用超順磁性氧化鐵(SPIO)標記的神經榦細胞(NSCs)在放射性腦損傷大鼠腦的磁共振(MR)顯像及遷移.方法 從大鼠胎鼠腦組織中分離、培養NSCs.採用nestin抗原錶達鑒定培養的NSCs.採用電子線半腦照射法製作大鼠放射損傷模型.用SPIO標記NSCs,根據大鼠腦立體定位圖譜,將NSCs植入大鼠腦內.行MR檢查,瞭解榦細胞的成像情況及在放射後大鼠腦內的分佈遷移.結果 分離、培養的細胞錶達nestin.標記的NSCs可以在MR顯像,成像可以持續1箇月以上.移植術後可以髮現榦細胞嚮放射部位的遷移.結論 MR可以顯示榦細胞嚮放射損傷部位的定嚮遷移.
목적 탐토용초순자성양화철(SPIO)표기적신경간세포(NSCs)재방사성뇌손상대서뇌적자공진(MR)현상급천이.방법 종대서태서뇌조직중분리、배양NSCs.채용nestin항원표체감정배양적NSCs.채용전자선반뇌조사법제작대서방사손상모형.용SPIO표기NSCs,근거대서뇌입체정위도보,장NSCs식입대서뇌내.행MR검사,료해간세포적성상정황급재방사후대서뇌내적분포천이.결과 분리、배양적세포표체nestin.표기적NSCs가이재MR현상,성상가이지속1개월이상.이식술후가이발현간세포향방사부위적천이.결론 MR가이현시간세포향방사손상부위적정향천이.
Objective To exploring the distribution and immigration of cells labeled with superparamagnetic iron oxide in irradiated brain of rats.Methods The NSCs were isolated from brain of fetal rat,cultured and stained with nestin antibody.The model of radiated rats brain were established by electron beam irradiation.The NSCs were labeled with SPIO and the labeled cell was transplanted to the irradiated brain of rats according to stereotaxic atlas of the rat beain.MRI was performed to evaluate the distribution and immigration of labeled cell in irradiated brain.Result The NSCs were positive for nestin after culture.They can been detected by MR imaging for at least one month.The radiation injury can induce the migration of NSCs to brain injured by irradiation.Conclusion MR can detect the immigration of labeled cell to the injured brain of rats.
