中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2010年
10期
797-800
,共4页
王学强%方益锋%吕和平%单云峰%张启瑜
王學彊%方益鋒%呂和平%單雲峰%張啟瑜
왕학강%방익봉%려화평%단운봉%장계유
胰腺肿瘤%基因疗法%腺病毒,人%内皮抑素类
胰腺腫瘤%基因療法%腺病毒,人%內皮抑素類
이선종류%기인요법%선병독,인%내피억소류
Pancreatic neoplasms%Gene therapy%Adenovirus,human%Endostatin
目的 观察携带人内皮抑素基因的双重调控增殖型腺病毒(AdTPHre-hEndo)对胰腺癌的治疗作用.方法 通过病毒重组技术将人内皮抑素基因克隆入双重调控增殖型腺病毒基因组中,获得腺病毒滴度为3.25×1010pfu/ml;通过建立SW-1990胰腺癌细胞Balb/c裸鼠皮下移植瘤模型,分析基因转导后胰腺癌组织中内皮抑素的表达情况及对肿瘤血管的抑制作用.结果 构建了AdTPHre-hEndo;AdTPHre-hEndo组荷瘤裸鼠肿瘤体积显著低于携带人内皮抑素基因的重组腺病毒(Ad-hEndo)组(P＜0.01)和选择性增殖型腺病毒(ONYX-015)组(P＜0.05);AdTPHre-hEndo组内皮抑素表达量明显高于Ad-hEndo组和对照组(P＜0.01).AdTPHre-hEndo组肿瘤微血管密度(MVD)为6.8±2.5,Ad-hEndo组和对照组MVD分别为16.0±4.6(P＜0.01)、47.2±10.0(P＜0.01).结论 所构建的AdTPHre-hEndo可有效表达具有生物学活性的内皮抑素,使肿瘤内微血管生成减少,肿瘤细胞增殖减慢,具备应用于胰腺癌临床治疗的潜力.
目的 觀察攜帶人內皮抑素基因的雙重調控增殖型腺病毒(AdTPHre-hEndo)對胰腺癌的治療作用.方法 通過病毒重組技術將人內皮抑素基因剋隆入雙重調控增殖型腺病毒基因組中,穫得腺病毒滴度為3.25×1010pfu/ml;通過建立SW-1990胰腺癌細胞Balb/c裸鼠皮下移植瘤模型,分析基因轉導後胰腺癌組織中內皮抑素的錶達情況及對腫瘤血管的抑製作用.結果 構建瞭AdTPHre-hEndo;AdTPHre-hEndo組荷瘤裸鼠腫瘤體積顯著低于攜帶人內皮抑素基因的重組腺病毒(Ad-hEndo)組(P＜0.01)和選擇性增殖型腺病毒(ONYX-015)組(P＜0.05);AdTPHre-hEndo組內皮抑素錶達量明顯高于Ad-hEndo組和對照組(P＜0.01).AdTPHre-hEndo組腫瘤微血管密度(MVD)為6.8±2.5,Ad-hEndo組和對照組MVD分彆為16.0±4.6(P＜0.01)、47.2±10.0(P＜0.01).結論 所構建的AdTPHre-hEndo可有效錶達具有生物學活性的內皮抑素,使腫瘤內微血管生成減少,腫瘤細胞增殖減慢,具備應用于胰腺癌臨床治療的潛力.
목적 관찰휴대인내피억소기인적쌍중조공증식형선병독(AdTPHre-hEndo)대이선암적치료작용.방법 통과병독중조기술장인내피억소기인극륭입쌍중조공증식형선병독기인조중,획득선병독적도위3.25×1010pfu/ml;통과건립SW-1990이선암세포Balb/c라서피하이식류모형,분석기인전도후이선암조직중내피억소적표체정황급대종류혈관적억제작용.결과 구건료AdTPHre-hEndo;AdTPHre-hEndo조하류라서종류체적현저저우휴대인내피억소기인적중조선병독(Ad-hEndo)조(P＜0.01)화선택성증식형선병독(ONYX-015)조(P＜0.05);AdTPHre-hEndo조내피억소표체량명현고우Ad-hEndo조화대조조(P＜0.01).AdTPHre-hEndo조종류미혈관밀도(MVD)위6.8±2.5,Ad-hEndo조화대조조MVD분별위16.0±4.6(P＜0.01)、47.2±10.0(P＜0.01).결론 소구건적AdTPHre-hEndo가유효표체구유생물학활성적내피억소,사종류내미혈관생성감소,종류세포증식감만,구비응용우이선암림상치료적잠력.
Objective To investigate the effect of a dual regulation of replicating adenovirus vector carrying human endostatin gene (AdTPHre-hEndo) on pancreatic cancer. Methods Human endostatin (hEndo) gene was cloned into the genome of replicating adenovirus specific for the tumor cells by virus recombination technology. The virus titer was 3.25 × 1010pfu/ml. A Balb/c nude mouse model carring sw1990 cells pancreatic cancer was established, the expression of human endostain and inhibition of tumor cells in vivo were detected. Results We successfully constructed AdTPHre-hEndo. The inhibition on pancreatic cancer cell line SW-1990 of AdTPHre-hEndo is better than Ad-hEndo (P ＜0. 01 ), and ONYX-015 ( P ＜ 0. 05 ). The endostatin expression of AdTPHre-hEndo group was significantly higher than Ad-hEndo group and the control group (P ＜ 0. 01 ). The intratumoral MVD also decreased significantly in the treated tumors(6. 8 ±2. 5 vs. 16. 0 ±4. 6、47. 2 ± 10. 0, P ＜0. 01 ). Conclusions The recombination adenovirus can express biologically active hEndo effectively, which results in inhibiting the growth of micro-blood vessels and proliferation slowly.
