中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2011年
12期
1043-1046
,共4页
树突细胞%血管内皮生长因子类%抗原,分化%免疫耐受
樹突細胞%血管內皮生長因子類%抗原,分化%免疫耐受
수돌세포%혈관내피생장인자류%항원,분화%면역내수
Dendritic cells%Vascular endothelial growth factors%Antigens,differentiation%Immune tolerance
目的 观察小鼠肺脏基质细胞分泌的血管内皮生长因子(VEGF)对成熟树突状细胞(mDC)分化和功能及免疫耐受的影响.方法 建立肺脏基质细胞/树突状细胞共培养体系作为对照组,体系中加入VEGF抗体作为实验组(VEGF抗体组).反转录聚合酶链反应法检测肺脏基质细胞微环境中细胞因子的表达；透射电镜观察细胞形态.流式细胞术检测细胞表型；CCK-8法检测树突状细胞诱导T细胞增殖的能力.结果 VEGF抗体组树突状细胞表型CD86表达与对照组相比明显上调(P＜0.05),诱导T细胞增殖的能力差异无统计学意义(P＞0.05).结论 VEGF通过下调CD86的表达参与树突状细胞诱导免疫耐受的过程.
目的 觀察小鼠肺髒基質細胞分泌的血管內皮生長因子(VEGF)對成熟樹突狀細胞(mDC)分化和功能及免疫耐受的影響.方法 建立肺髒基質細胞/樹突狀細胞共培養體繫作為對照組,體繫中加入VEGF抗體作為實驗組(VEGF抗體組).反轉錄聚閤酶鏈反應法檢測肺髒基質細胞微環境中細胞因子的錶達；透射電鏡觀察細胞形態.流式細胞術檢測細胞錶型；CCK-8法檢測樹突狀細胞誘導T細胞增殖的能力.結果 VEGF抗體組樹突狀細胞錶型CD86錶達與對照組相比明顯上調(P＜0.05),誘導T細胞增殖的能力差異無統計學意義(P＞0.05).結論 VEGF通過下調CD86的錶達參與樹突狀細胞誘導免疫耐受的過程.
목적 관찰소서폐장기질세포분비적혈관내피생장인자(VEGF)대성숙수돌상세포(mDC)분화화공능급면역내수적영향.방법 건립폐장기질세포/수돌상세포공배양체계작위대조조,체계중가입VEGF항체작위실험조(VEGF항체조).반전록취합매련반응법검측폐장기질세포미배경중세포인자적표체；투사전경관찰세포형태.류식세포술검측세포표형；CCK-8법검측수돌상세포유도T세포증식적능력.결과 VEGF항체조수돌상세포표형CD86표체여대조조상비명현상조(P＜0.05),유도T세포증식적능력차이무통계학의의(P＞0.05).결론 VEGF통과하조CD86적표체삼여수돌상세포유도면역내수적과정.
Objective To observe the effects of vascular endothelial growth factor(VEGF)from pulmonary stromal cells on differentiation and function of mature dendritic cells (mDC) and the immune tolerance. Methods Co-culture system of murine pulmonary stroma cells (MPSC)/mDC was established as control group,and VEGF-Ab add-on treatment to co-culture cells as experimental group.Cytokines secreted from pulmonary stroma cells were detected by RT-PCR.The expression of cellular phenotype and ability of the mDC-induced T cells reproductive activity were detected by flow cytometry and CCK-8,respectively.Results CD86 expression of VEGF-Ab group was higher than of control group (P ＜ 0.05).There was no difference in the ability of mDC-induced T cells reproductive activity between the two groups(P＞0.05).Conclusions VEGF might be involved in the regulation of immune tolerance by reducing expression of CD86 in dendritic cells.
