中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
10期
1662-1664
,共3页
彭艳%谢伟%汪付兵%阳芳%柳琨%方喜平%段丽%吴红艳%冯茂辉
彭豔%謝偉%汪付兵%暘芳%柳琨%方喜平%段麗%吳紅豔%馮茂輝
팽염%사위%왕부병%양방%류곤%방희평%단려%오홍염%풍무휘
癌,肝细胞%唾液酸化LewisX抗原%DNA适配子%侵袭
癌,肝細胞%唾液痠化LewisX抗原%DNA適配子%侵襲
암,간세포%타액산화LewisX항원%DNA괄배자%침습
Carcinoma,hepatocellular%Sialyl LweisX%DNA aptamer%Invasion
目的 观察特异结合唾液酸化LewisX (SLeX)抗原DNA适配子抑制HepG2细胞与E-选择素黏附能力及其体外抑制HepG2细胞浸润转移能力.方法 采用黏附实验、Transwell体外侵袭实验,检测该适配子对HepG2细胞与E选择素黏附及对HepG2细胞体外侵袭的影响.结果 该DNA适配子可有效抑制HepG2细胞与E-选择素黏附,黏附细胞数随适配子浓度的增加而减少(P＜0.01),20 nmol浓度的适配子与单克隆抗体CSLEX-1效果相似；Transwell侵袭实验中,5、10、20nmol适配子组的侵袭细胞数分别为159.00±3.27、142.00±5.50、115.00±5.07,与对照组(178.00±4.64)比较,差异有统计学意义(P＜0.01).结论 特异结合唾液酸化LewisX抗原DNA适配子可以抑制HepG2细胞与E-选择素黏附,阻断Lewis-selectin途径,抑制HepG2细胞体外侵袭转移.
目的 觀察特異結閤唾液痠化LewisX (SLeX)抗原DNA適配子抑製HepG2細胞與E-選擇素黏附能力及其體外抑製HepG2細胞浸潤轉移能力.方法 採用黏附實驗、Transwell體外侵襲實驗,檢測該適配子對HepG2細胞與E選擇素黏附及對HepG2細胞體外侵襲的影響.結果 該DNA適配子可有效抑製HepG2細胞與E-選擇素黏附,黏附細胞數隨適配子濃度的增加而減少(P＜0.01),20 nmol濃度的適配子與單剋隆抗體CSLEX-1效果相似；Transwell侵襲實驗中,5、10、20nmol適配子組的侵襲細胞數分彆為159.00±3.27、142.00±5.50、115.00±5.07,與對照組(178.00±4.64)比較,差異有統計學意義(P＜0.01).結論 特異結閤唾液痠化LewisX抗原DNA適配子可以抑製HepG2細胞與E-選擇素黏附,阻斷Lewis-selectin途徑,抑製HepG2細胞體外侵襲轉移.
목적 관찰특이결합타액산화LewisX (SLeX)항원DNA괄배자억제HepG2세포여E-선택소점부능력급기체외억제HepG2세포침윤전이능력.방법 채용점부실험、Transwell체외침습실험,검측해괄배자대HepG2세포여E선택소점부급대HepG2세포체외침습적영향.결과 해DNA괄배자가유효억제HepG2세포여E-선택소점부,점부세포수수괄배자농도적증가이감소(P＜0.01),20 nmol농도적괄배자여단극륭항체CSLEX-1효과상사；Transwell침습실험중,5、10、20nmol괄배자조적침습세포수분별위159.00±3.27、142.00±5.50、115.00±5.07,여대조조(178.00±4.64)비교,차이유통계학의의(P＜0.01).결론 특이결합타액산화LewisX항원DNA괄배자가이억제HepG2세포여E-선택소점부,조단Lewis-selectin도경,억제HepG2세포체외침습전이.
Objective To study the effect of the Sialyl LewisX (SLeX) binding DNA aptamer on adhesion and metastasis of hepatocellular carcinoma (HCC) HepG2 cells in vitro,and to select possible anti-adhesion drugs for HCC metastasis.Methods The adhesive potential of HepG2 cells to E-selectin and alterations of the adhesive potential after HepG2 cells were sealed by the SLeX binding DNA aptamer were studied by means of the adhesive test.The effect of DNA aptamer on the metastasis of HepG2 cells was evaluated by tumor cell migration invasion assays.Results Most of the interactions between E-selectin and the SLeX on the HepG2 cells are specific and could be inhibited by the SLeX binding DNA aptamer.Cell number on the down-side of transwell membrane was significantly less in cells treated with 5,10,20 nmol DNA aptamer than in the control cells ( 159.00 ±3.27,142.00±5.50 and 115.00 ±5.07,vs.178.00 ±4.64,P ＜0.01 ).Conclusion These results indicate that the SLeX binding DNA aptamer can effectively and significantly inhibit the in vitroadhesion,migration,and invasion of HepG2 cells,and also suggest that the SLeX binding DNA aptamer may have potential as an agent to inhibit cancer growth and metastasis.
