中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
12期
1065-1068
,共4页
陆丽明%邝枣园%罗振亮%林洁涛%陈晓瑜%曾婧纯
陸麗明%鄺棘園%囉振亮%林潔濤%陳曉瑜%曾婧純
륙려명%광조완%라진량%림길도%진효유%증청순
黄连素%巨噬细胞凋亡%动脉粥样硬化
黃連素%巨噬細胞凋亡%動脈粥樣硬化
황련소%거서세포조망%동맥죽양경화
Bererine%Macrophages apoptosis%Atherosclerosis
目的 研究黄连素抗小鼠巨噬细胞凋亡的作用,初步确定其抗凋亡的浓度,探讨其治疗动脉粥样硬化(athemsclemsis,As)的机制.方法 以0.5 μmol/L的毒胡萝卜素(Tg)和50 μg/ml的墨角藻聚糖(Fuc)诱导RAW264.7巨噬细胞凋亡为实验模型,通过细胞毒性试验(MTT)法检测细胞存活率,Annexin V-FITC/P1流式细胞术检测细胞凋亡率.结果 浓度为800 μg/ml的黄连素组与正常对照组细胞存活率相比差异有统计学意义(P<0.01);随着黄连素浓度的下降,细胞凋亡率旱现下降的趋势,黄连素(0.02~0.63 μg/ml)能有效抑制Tg和Fuc诱导的小鼠巨噬细胞凋亡.结论 黄连素对RAW267.4细胞的增殖影响不大.黄连素能有效抑制Tg和Fuc诱导的小鼠巨噬细胞凋亡.
目的 研究黃連素抗小鼠巨噬細胞凋亡的作用,初步確定其抗凋亡的濃度,探討其治療動脈粥樣硬化(athemsclemsis,As)的機製.方法 以0.5 μmol/L的毒鬍蘿蔔素(Tg)和50 μg/ml的墨角藻聚糖(Fuc)誘導RAW264.7巨噬細胞凋亡為實驗模型,通過細胞毒性試驗(MTT)法檢測細胞存活率,Annexin V-FITC/P1流式細胞術檢測細胞凋亡率.結果 濃度為800 μg/ml的黃連素組與正常對照組細胞存活率相比差異有統計學意義(P<0.01);隨著黃連素濃度的下降,細胞凋亡率旱現下降的趨勢,黃連素(0.02~0.63 μg/ml)能有效抑製Tg和Fuc誘導的小鼠巨噬細胞凋亡.結論 黃連素對RAW267.4細胞的增殖影響不大.黃連素能有效抑製Tg和Fuc誘導的小鼠巨噬細胞凋亡.
목적 연구황련소항소서거서세포조망적작용,초보학정기항조망적농도,탐토기치료동맥죽양경화(athemsclemsis,As)적궤제.방법 이0.5 μmol/L적독호라복소(Tg)화50 μg/ml적묵각조취당(Fuc)유도RAW264.7거서세포조망위실험모형,통과세포독성시험(MTT)법검측세포존활솔,Annexin V-FITC/P1류식세포술검측세포조망솔.결과 농도위800 μg/ml적황련소조여정상대조조세포존활솔상비차이유통계학의의(P<0.01);수착황련소농도적하강,세포조망솔한현하강적추세,황련소(0.02~0.63 μg/ml)능유효억제Tg화Fuc유도적소서거서세포조망.결론 황련소대RAW267.4세포적증식영향불대.황련소능유효억제Tg화Fuc유도적소서거서세포조망.
Objective To investigate the effect of berberine anti macrophages apoptosis, initially to determine the concentration of anti-apoptosis and to explore its mechanisms of treatment in atherosclerosis (AS). Methods Apoptosis of RAW264.7 macrophages is induced by 0.5 μmol/L Tg and 50 μg/ml Fuc. The cell survival rate and apoptosis rate was detected by MIT and flow cytometry, respectively. Results There was statistical significance between the berberine group of 800 μg/ml and normal group in the cell sur-vival rate(P<0.01). With the decline in concentration of berberine, the cell apoptosis rate showed a down-ward trend, berberine (0.02-0.63 μg/ml) effectively inhibited the cell apoptosis induced by Tg and Fuc. Conclusion Berberine group has little impact on the cell proliferation. Berberine can effectively inhibit the cell apoptosis induced by Tg and Fuc.