CAJ | 학술논문

本文研究心房特殊颗粒(ASG)中的钙释放是否能促进颗粒内容的分泌.冷冻超薄切片技术和磷钨酸-乙醇(EPTA)特殊染色技术制备大鼠心房切片.X射线显微定量分析采用Hall连续谱法,用以测量在肾上腺素处理后15、30、60和120 min时ASG中钙含量的变化.用形态计量法的数密度作为判断ASG分泌状态的指标.结果显示心房特殊颗粒在生理状态下钙浓度高达80 mmol/公斤*干重.在肾上腺素注射后ASG的数密度逐渐下降,并于注射后60 min时下降至最低;ASG中的钙含量亦逐渐减低,但于注射后30 min即达最低,由此可见ASG钙含量的下降出现早于颗粒数的下降.推想肾上腺素处理引起的钙释放可能成为一种信号,启动心房肽的分泌,因此ASG在心房心肌细胞中可能具有细胞内钙库机能.本文同时讨论了Hall连续谱法近年来在生物试样定量分析中的应用和发展.
본문연구심방특수과립(ASG)중적개석방시부능촉진과립내용적분비.냉동초박절편기술화린오산-을순(EPTA)특수염색기술제비대서심방절편.X사선현미정량분석채용Hall련속보법,용이측량재신상선소처리후15、30、60화120 min시ASG중개함량적변화.용형태계량법적수밀도작위판단ASG분비상태적지표.결과현시심방특수과립재생리상태하개농도고체80 mmol/공근*간중.재신상선소주사후ASG적수밀도축점하강,병우주사후60 min시하강지최저;ASG중적개함량역축점감저,단우주사후30 min즉체최저,유차가견ASG개함량적하강출현조우과립수적하강.추상신상선소처리인기적개석방가능성위일충신호,계동심방태적분비,인차ASG재심방심기세포중가능구유세포내개고궤능.본문동시토론료Hall련속보법근년래재생물시양정량분석중적응용화발전.
Whether the release of Ca from atrial specific granules (ASG) can promote ASG secretion was studied. The ultrathin cryosections and ethanolic phospho tungstic acid staining sections of rat atrial cardiocytes were prepared. Quantit ative electron probe Xray microanalysis of Hall continuum method was adopted to measure the alteration of Ca content in ASG at the exact time intervals of 15, 30, 60, 120 min after the adrenaline (Ad) administration. The numerical density (Nv) was measured to estimate the ASG secretion. It was observed that the Nv of ASG gradually decreased and reached the lowest value at the time of 60 min after the Ad administration. Meanwhile, the Ca content of ASG gradually decreased and reached the lowest value at the time of 30 min after the Ad administration. It showed that the decrease in Ca content took place earlier than that of ASG number. Accordingly, it suggested that the Ca released from ASG due to Ad stimulation might become a messenger triggering atrial natriuretic peptide secretion. The quantitative electron probe Xray microananlysis of Hall continuum method was discussed in this paper.