华人消化杂志
華人消化雜誌
화인소화잡지
WORLD CHINESE JOURNAL OF DIESTION
1998年
8期
647-649
,共3页
崔龙%曹广文%王元和%屠岳%孟荣贵%高军%仇小芳%吴宗娣
崔龍%曹廣文%王元和%屠嶽%孟榮貴%高軍%仇小芳%吳宗娣
최룡%조엄문%왕원화%도악%맹영귀%고군%구소방%오종제
herpes simplex virus%thymidine kinase gene%colorectal carcinoma%transcriptional regulatory sequences
AIM In order to express suicide gene specifically in colorectal carcinoma cells for targeted gene therapy, we constructed the recombinant retroviral vector promoted by transcriptional regulatory sequences of carcinoembryonic antigen (CEA) gene.METHODS A 1.3 kb EcoRⅠ/ BamHⅠ fragment of the HSV-tk gene was isolated from pLNtk and inserted into pUC118, then the HindⅢ/ BamHⅠ fragment from pCEA424/ 2CAT was isolated and subcloned into pUC118 to harvest HindⅢ/ EcoRⅠ CEA TRS. These two fragments were ligated with the retroviral vector GINa.RESULTS Restriction enzyme Sal Ⅰwas used to identify a positive clone B5. The recombinant retroviral vector, GICEAtkNa was constructed.CONCLUSION Success in construction of GICEAtkNa provides a basis for colorectal carcinoma-specific gene therapy.
