天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2009年
4期
577-580
,共4页
刘浩%张娜%阴春晖%王蓟花%马占鸿%周立刚
劉浩%張娜%陰春暉%王薊花%馬佔鴻%週立剛
류호%장나%음춘휘%왕계화%마점홍%주립강
抗真菌化合物%带毒平板法%液体培养法%植物病原真菌%菌丝生长
抗真菌化閤物%帶毒平闆法%液體培養法%植物病原真菌%菌絲生長
항진균화합물%대독평판법%액체배양법%식물병원진균%균사생장
antifungal compound%radial growth assay%liquid suspension culture assay%plant pathogenic fungus%mycelial growth
采用带毒平板法和液体培养法测定两种植物抗菌化合物血根碱和白屈菜红碱对植物病原真菌水稻纹枯病菌(Rhizoctonia solani)、番茄枯萎病菌(Fusarium oxysporium f. sp.lycopersici)和棉花枯萎病菌(F.oxysporium f.sp.vasinfectum)的抑制活性.论文对两种测定方法进行了比较和讨论,结果发现:和带毒平板法相比,采用液体培养法获得的化合物半抑制浓度要低.
採用帶毒平闆法和液體培養法測定兩種植物抗菌化閤物血根堿和白屈菜紅堿對植物病原真菌水稻紋枯病菌(Rhizoctonia solani)、番茄枯萎病菌(Fusarium oxysporium f. sp.lycopersici)和棉花枯萎病菌(F.oxysporium f.sp.vasinfectum)的抑製活性.論文對兩種測定方法進行瞭比較和討論,結果髮現:和帶毒平闆法相比,採用液體培養法穫得的化閤物半抑製濃度要低.
채용대독평판법화액체배양법측정량충식물항균화합물혈근감화백굴채홍감대식물병원진균수도문고병균(Rhizoctonia solani)、번가고위병균(Fusarium oxysporium f. sp.lycopersici)화면화고위병균(F.oxysporium f.sp.vasinfectum)적억제활성.논문대량충측정방법진행료비교화토론,결과발현:화대독평판법상비,채용액체배양법획득적화합물반억제농도요저.
Inhibitory activities of sanguinarine and chelerythrine on the mycelial growth of plant pathogenic fungi (i. e. Rhizoctonia solani ,Fusarium oxysporium f. sp. lycopersici and F. oxysporium f. sp. vasinfectum) were screened by using both radial growth assay and liquid suspension culture assay. The two assays for screening of antifungal activity of natural compounds on mycelial growth were compared and discussed. As a result, median inhibitory concentration (IC_(50)) values of the compounds obtained by liquid suspension culture assay were lower than those by radial growth assay.