CAJ | 학술논문

背景:骨组织中整合素表达水平可影响细胞功能,调控成骨和骨吸收,但整合索只有被其他因于激活后才能发挥强大的黏附作用,重组人骨形成蛋白2具有此作用吗?目的:观察不同质量重组人骨形成蛋白2对兔下颌骨牵引成骨区整合素B_1的表达影响.设计、时间及地点:随机分组动物实验,组织学观察,于2006-05/2007-05在辽宁医学院动物实验中心和免疫组化中心完成.材料:日本成年大耳白兔45只.重组人骨形成蛋白2由北京军事医学科学院三所八室提供.方法:选用日本成年大耳白兔45只建立下颌骨牵引模型,造模后采用随机数表法将分为3组:空白对照组、1.5,3.0 mg重组人骨形成蛋白2胶原海绵载体组,15只/组.分别将不含重组人骨形成蛋白2胶原海绵载体、含1.5,3.0 mg重组人骨形成蛋白胶原复合物植入3组兔下颌骨切开处,固定、牵引0.4 mm/次,2次/d,共计5 d,总延长下颌骨4 mm.主要观察指标:牵引后稳定期第1,3,7,14,28天取牵引区新生骨痂行免疫组织化学染色观察骨组织中整合素β_1的表达.结果:45只日本大耳白兔均建模成功,进入结果分析.整合素β_1在牵引区表达主要反映在牵引7 d后.在同一时间内,随着重组人骨形成蛋白2质量的增加,整合素β_1阳性细胞表达率、阳性面积百分比逐渐增多(P<0.05):在同一质量下,随着时间的延长,整合素β_1阳性细胞阳性表达率、阳性面积百分比逐渐上升(P<0.05).结论:局部应用外源性重组人骨形成蛋白2在牵引成骨中晚期对整合素β_1的表达有促进作用,并且整合素β_1的表达对重组人骨形成蛋白2呈质量依赖性.
배경:골조직중정합소표체수평가영향세포공능,조공성골화골흡수,단정합색지유피기타인우격활후재능발휘강대적점부작용,중조인골형성단백2구유차작용마?목적:관찰불동질량중조인골형성단백2대토하합골견인성골구정합소B_1적표체영향.설계、시간급지점:수궤분조동물실험,조직학관찰,우2006-05/2007-05재료녕의학원동물실험중심화면역조화중심완성.재료:일본성년대이백토45지.중조인골형성단백2유북경군사의학과학원삼소팔실제공.방법:선용일본성년대이백토45지건립하합골견인모형,조모후채용수궤수표법장분위3조:공백대조조、1.5,3.0 mg중조인골형성단백2효원해면재체조,15지/조.분별장불함중조인골형성단백2효원해면재체、함1.5,3.0 mg중조인골형성단백효원복합물식입3조토하합골절개처,고정、견인0.4 mm/차,2차/d,공계5 d,총연장하합골4 mm.주요관찰지표:견인후은정기제1,3,7,14,28천취견인구신생골가행면역조직화학염색관찰골조직중정합소β_1적표체.결과:45지일본대이백토균건모성공,진입결과분석.정합소β_1재견인구표체주요반영재견인7 d후.재동일시간내,수착중조인골형성단백2질량적증가,정합소β_1양성세포표체솔、양성면적백분비축점증다(P<0.05):재동일질량하,수착시간적연장,정합소β_1양성세포양성표체솔、양성면적백분비축점상승(P<0.05).결론:국부응용외원성중조인골형성단백2재견인성골중만기대정합소β_1적표체유촉진작용,병차정합소β_1적표체대중조인골형성단백2정질량의뢰성.
BACKGROUND:Integfin levels can affect cellular function,regulate bone formation and bone resorption.However,this process need activated by other factors,whether recombinant human bone morphogenetic protein 2 (rhBMP-2)has this effect is poorly understood.OBJECTIVE:To study the effects of the different concentration of rhBMP-2 on the expression of inergrin β_1 during the mandibular dfScraction in rabbits.DESIGN,TIME AND SETTING:The randomized,controlled.animal grouping experiment of histological observation was performed at the Animal ExperimentaI Center and Immunohistochemistry Center of Liaoning Medical University from May 2006 to May 2007.MATERIALS:A total of 45 Japanese white rabbits were used.rhBMP-2 was supplied by Beijing Academy of Military Medical Sciences.METHODS:The rabbits were prepared for mandibular distraction models and randomly assigned into control,1.5 mg rhBMP-2 and 3.0 mg rhBMP-2 groups.with 15 animals in each group.Collagen sponge and collagen binding proteins were implanted into mandibles of rabbits,respectively,and distraction 0.4 mm per time,twice per day for 5 days.The mandibles were lengthened 4 mm.MAIN OUTCOME MEASURES:The expression of integrin 131 was detected using immunohistochemistry at days 1,3,7,14,and 28 after distraction.RESULTS:All rabbits were included in the final analysis.The integdn β_1 mainly expressed at 7 days after distraction.At the same time point.when concenrtation rhBMP-2 was increased,the positive integrin β_1 rate and positive stained area percentage were increased(P<0.05).Compared to the same concentration,the positive integrin β_1 rate and positive stained area percentage were gradually increased as time prolonged (P<0.05).CONCLUSION:The integdn β_1 expression can be promoted by rhBMP-2 at the anaphase of mandibular distraction osteogenesis in a concentration-dependent manner.