中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2011年
5期
397-402
,共6页
孙大康%安新业%徐殿红%赵延婷%王健%阮月芹
孫大康%安新業%徐殿紅%趙延婷%王健%阮月芹
손대강%안신업%서전홍%조연정%왕건%원월근
三基序蛋白22%核因子κB%生物信息学%双萤光素酶报告基因系统
三基序蛋白22%覈因子κB%生物信息學%雙螢光素酶報告基因繫統
삼기서단백22%핵인자κB%생물신식학%쌍형광소매보고기인계통
Trimotif protein 22%Nuclear factor-kB%Bioinformatics%Dual-luciferase reporter gene system
目的 通过生物信息学方法寻找Trim30α的人同源基因,观察候选Trim蛋白对TAB2诱导的NF-κB荧光素酶报告基因活性的影响.方法 经MEGA4软件对具有RBCC和SPRY结构域的Trim分子行进化树分析,用DNAMAN 6.0软件对候选Trim与Trim30α进行氨基酸序列比对,比较Trim30α相毗邻基因在小鼠和人染色体上的排列分布规律.构建Trim22的真核表达载体,用双萤光素酶报告基因系统检测Trim22对TAB2诱导的NF- κB报告基因活化的影响.结果 Trim30α与人Trim5α.、Trim6、Trim22、Trim34α来自进化树的同一分支节点；Trim22、Trim5α与Trim30α的氨基酸序列同源性分别为42.51％、45.47％；Trim22可以抑制TAB2诱导的NF-κB报告基因的活化,而Trim5α无显著抑制作用.结论 Trim22对TAB2诱导的NF-κB报告基因活化有负向调节作用,可能与Trim30α具有相类似的生物学功能.
目的 通過生物信息學方法尋找Trim30α的人同源基因,觀察候選Trim蛋白對TAB2誘導的NF-κB熒光素酶報告基因活性的影響.方法 經MEGA4軟件對具有RBCC和SPRY結構域的Trim分子行進化樹分析,用DNAMAN 6.0軟件對候選Trim與Trim30α進行氨基痠序列比對,比較Trim30α相毗鄰基因在小鼠和人染色體上的排列分佈規律.構建Trim22的真覈錶達載體,用雙螢光素酶報告基因繫統檢測Trim22對TAB2誘導的NF- κB報告基因活化的影響.結果 Trim30α與人Trim5α.、Trim6、Trim22、Trim34α來自進化樹的同一分支節點；Trim22、Trim5α與Trim30α的氨基痠序列同源性分彆為42.51％、45.47％；Trim22可以抑製TAB2誘導的NF-κB報告基因的活化,而Trim5α無顯著抑製作用.結論 Trim22對TAB2誘導的NF-κB報告基因活化有負嚮調節作用,可能與Trim30α具有相類似的生物學功能.
목적 통과생물신식학방법심조Trim30α적인동원기인,관찰후선Trim단백대TAB2유도적NF-κB형광소매보고기인활성적영향.방법 경MEGA4연건대구유RBCC화SPRY결구역적Trim분자행진화수분석,용DNAMAN 6.0연건대후선Trim여Trim30α진행안기산서렬비대,비교Trim30α상비린기인재소서화인염색체상적배렬분포규률.구건Trim22적진핵표체재체,용쌍형광소매보고기인계통검측Trim22대TAB2유도적NF- κB보고기인활화적영향.결과 Trim30α여인Trim5α.、Trim6、Trim22、Trim34α래자진화수적동일분지절점；Trim22、Trim5α여Trim30α적안기산서렬동원성분별위42.51％、45.47％；Trim22가이억제TAB2유도적NF-κB보고기인적활화,이Trim5α무현저억제작용.결론 Trim22대TAB2유도적NF-κB보고기인활화유부향조절작용,가능여Trim30α구유상유사적생물학공능.
Objective To screen for human Trim30a homologous gene via bioinformatic approaches and to investigate the impact of Trim protein candidates on TAB2-induced NF-kB luciferase reporter gene activity.Methods Phylogenetic tree of Trim molecules containing RBCC and SPRY domains was analyzed using MEGA4 software.The amino acid sequences of Trim candidates and Trim30a were compared using DNAMAN 6.0 for further comparison on the sequence and distribution of Trim30a proximal genes in human and murine chromosomes.Trim22 eukaryotic expression vector was constructed,and the impact of Trim22 on TAB2- induced NF-kB reporter gene activity was determined using dual- luciferase reporter gene system.Results Phylogenetic tree analysis showed that Trim30a originated from the identical bifurcation point with human Trim5a,Trim6,Trim22 and Trim34a.The amino acid sequence of Trim22 and Trim5a exhibited homogeneity of 42.51％ and 45.47％ with that of Trim30a,respectively.Trim22,but not Trim5a,inhibited TAB2-induced NF-kB reporter gene activation.Conclusion Trim22 is involved in negative regulation of TAB2-induced NF-kB reporter gene activation and may share similar biological properties with Trim30a.
