中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2012年
1期
1-4
,共4页
杨少波%郑继翠%肖现民%郑珊%徐挺
楊少波%鄭繼翠%肖現民%鄭珊%徐挺
양소파%정계취%초현민%정산%서정
神经母细胞瘤%Oct-4转录因子
神經母細胞瘤%Oct-4轉錄因子
신경모세포류%Oct-4전록인자
Neuroblastoma%Octamer transcription factor-4
目的 探讨神经母细胞瘤组织中胚胎干细胞基因Oct-4的表达情况及其意义.方法 收集39例神经母细胞瘤和相应癌旁组织,应用实时定量PCR和Western印迹法检测Oct-4的表达,并分析其表达与临床病理参数的相关性.结果 术前未化疗的神经母细胞瘤Oct-4的mRNA相对表达量为6.53±1.74,明显强于瘤旁组织1.04±0.65,两组间差异有统计学意义(P＜0.05)；其中Ⅲ～Ⅳ期肿瘤Oct-4 mRNA相对表达量8.81±0.67较Ⅰ～Ⅱ期肿瘤4.26±0.54高,差异有统计学意义(P＜0.05).Ⅲ～Ⅳ期肿瘤中术前未化疗的Oct-4 mRNA相对表达量8.81±0.67明显高于术前化疗的肿瘤3.15±0.42(P＜0.05).神经母细胞瘤Oct-4表达与患儿性别、年龄、肿瘤大小、肿瘤部位、病理分型等参数无相关性.结论 Oct-4可能与神经母细胞瘤的发生、发展相关,化疗药物的使用对其表达有抑制作用.
目的 探討神經母細胞瘤組織中胚胎榦細胞基因Oct-4的錶達情況及其意義.方法 收集39例神經母細胞瘤和相應癌徬組織,應用實時定量PCR和Western印跡法檢測Oct-4的錶達,併分析其錶達與臨床病理參數的相關性.結果 術前未化療的神經母細胞瘤Oct-4的mRNA相對錶達量為6.53±1.74,明顯彊于瘤徬組織1.04±0.65,兩組間差異有統計學意義(P＜0.05)；其中Ⅲ～Ⅳ期腫瘤Oct-4 mRNA相對錶達量8.81±0.67較Ⅰ～Ⅱ期腫瘤4.26±0.54高,差異有統計學意義(P＜0.05).Ⅲ～Ⅳ期腫瘤中術前未化療的Oct-4 mRNA相對錶達量8.81±0.67明顯高于術前化療的腫瘤3.15±0.42(P＜0.05).神經母細胞瘤Oct-4錶達與患兒性彆、年齡、腫瘤大小、腫瘤部位、病理分型等參數無相關性.結論 Oct-4可能與神經母細胞瘤的髮生、髮展相關,化療藥物的使用對其錶達有抑製作用.
목적 탐토신경모세포류조직중배태간세포기인Oct-4적표체정황급기의의.방법 수집39례신경모세포류화상응암방조직,응용실시정량PCR화Western인적법검측Oct-4적표체,병분석기표체여림상병리삼수적상관성.결과 술전미화료적신경모세포류Oct-4적mRNA상대표체량위6.53±1.74,명현강우류방조직1.04±0.65,량조간차이유통계학의의(P＜0.05)；기중Ⅲ～Ⅳ기종류Oct-4 mRNA상대표체량8.81±0.67교Ⅰ～Ⅱ기종류4.26±0.54고,차이유통계학의의(P＜0.05).Ⅲ～Ⅳ기종류중술전미화료적Oct-4 mRNA상대표체량8.81±0.67명현고우술전화료적종류3.15±0.42(P＜0.05).신경모세포류Oct-4표체여환인성별、년령、종류대소、종류부위、병리분형등삼수무상관성.결론 Oct-4가능여신경모세포류적발생、발전상관,화료약물적사용대기표체유억제작용.
Objective To investigate the expression of Oct-4 in neuroblastoma.Methods The Oct-4 expression in 39 neuroblastoma tissues and paracancerous tissues was determined by real-time PCR and Western blot.The relationship between the expression of Oct-4 and clinical data was also analyzed.Results The expression level of Oct-4 in preoperative neuroblastoma tissues was significantly higher than that in paracancerous tissues(P＜0.05),and its expression in stage Ⅲ-Ⅳ neuroblastoma was higher than that in stage Ⅰ Ⅱ.In tumors of stage Ⅲ-Ⅳ,the Oct-4 expression was significantly decreased in the subgroup with preoperative chemotherapy when compared with that without preoperative chemotherapy.Conclusions The expression of Oct-4 correlates positively with the progression of neuroblastoma.The Oct-4 expression can be inhibited by chemotherapy.
