CAJ | 학술논문

目的探讨P53及METTL-9基因在结肠的正常或炎症组织,癌组织及癌旁组织中的表达情况,研究其与结肠癌发生发展的关系.方法将标本分为:正常或炎症组(5例正常和3例炎症结肠组织),癌组(22例)及癌旁组(22例).HE染色后在光学显微镜下观察组织细胞的形态学情况;应用实时荧光定量PCR方法检测各组织中P53和METTL-9基因的表达情况.结果正常或炎症组中P53基因的表达明显高于癌组和癌旁组(P＜0.05),癌组中P53基因的表达明显低于癌旁组(P＜0.05);正常或炎症组中METFL-9基因的表达明显低于癌组和癌旁组(P＜0.05),癌组织中METTL-9基因的表达明显高于癌旁组织(P＜0.05);癌症组织中P53基因与METTL-9基因呈负相关(P＜0.05).结论 P53基因的异常表达在结肠癌发生过程中起着重要的作用;其下游基因METTL-9在结肠癌中高表达,转录翻译生成的蛋白酶可能导致结肠癌中DNA高度甲基化,从而促进结肠癌的发生发展.
목적 탐토P53급METTL-9기인재결장적정상혹염증조직,암조직급암방조직중적표체정황,연구기여결장암발생발전적관계.방법 장표본분위:정상혹염증조(5례정상화3례염증결장조직),암조(22례)급암방조(22례).HE염색후재광학현미경하관찰조직세포적형태학정황;응용실시형광정량PCR방법 검측각조직중P53화METTL-9기인적표체정황.결과 정상혹염증조중P53기인적표체명현고우암조화암방조(P＜0.05),암조중P53기인적표체명현저우암방조(P＜0.05);정상혹염증조중METFL-9기인적표체명현저우암조화암방조(P＜0.05),암조직중METTL-9기인적표체명현고우암방조직(P＜0.05);암증조직중P53기인여METTL-9기인정부상관(P＜0.05).결론 P53기인적이상표체재결장암발생과정중기착중요적작용;기하유기인METTL-9재결장암중고표체,전록번역생성적단백매가능도치결장암중DNA고도갑기화,종이촉진결장암적발생발전.
Objective To explore the expression of P53 and mettl-9 gene in colon cancer tissues and inflammation tissues and normal adjacent tissues and study its relationship with colon cancer.Methods The morphologic situation of histiocyte after H.E.stained was observed.The real-time fluorescent quantitative PCR was applied to detect the gene expression of P53 and METFL-9.Results The expression of P53 gene in inflammation tissues was significantly higher than that in cancer tissues and adjacent tissues(P ＜0.05).The expression of P53 gene in cancer tissues was significantly lower than adjacent tissues and infiammation tissues.METTL-9 gene expression in inflammation tissues was significantly lower than cancer tissues and adjacent tissues(P ＜0.05).In cancer tissues,P53 gene and METFL-9 gene were negative related.Conclusions The mutations of P53 gene played an important role in the course of colon cancer,its downstream gene METTL-9 was highly expressed in colon cancer,and the 9-methyl-converting enzyme which produced by transcription and translation might lead gene hypermethylation in the colon cancer,and thereby promoted the development of colon cancer.