CAJ | 학술논문

目的对分离自中国东海30 m深海泥的一株产新型大环内酯抗生素(macrolactin s,MLS)的菌株进行形态、生理生化特性及分子水平的分类学研究.方法通过细菌培养特征观察,菌株生长条件测定、生理生化指标及全细胞脂肪酸成分分析,并特异性扩增其16S rDNA序列,测序比对及构建系统发育进化树,利用本实验室所建立的抗菌模型评价代谢产物的抗菌活性.结果该菌株与芽孢杆菌属其他种共享97%～99%的保守序列,其中与解淀粉芽孢杆菌(Bacillus amyloliquefaciens)的16SrDNA序列保守度高达99.6%.15:1 iso H/13:0 3OH为该菌株的主要脂肪酸组分.结论该菌株属于芽孢杆菌属,定名为解淀粉芽孢杆菌MS1721株,其所产大环内酯MLS表现出较强的抗菌活性.
목적 대분리자중국동해30 m심해니적일주산신형대배내지항생소(macrolactin s,MLS)적균주진행형태、생리생화특성급분자수평적분류학연구.방법 통과세균배양특정관찰,균주생장조건측정、생리생화지표급전세포지방산성분분석,병특이성확증기16S rDNA서렬,측서비대급구건계통발육진화수,이용본실험실소건립적항균모형평개대사산물적항균활성.결과 해균주여아포간균속기타충공향97%～99%적보수서렬,기중여해정분아포간균(Bacillus amyloliquefaciens)적16SrDNA서렬보수도고체99.6%.15:1 iso H/13:0 3OH위해균주적주요지방산조분.결론 해균주속우아포간균속,정명위해정분아포간균MS1721주,기소산대배내지MLS표현출교강적항균활성.
Objective To perform taxonomic studies on a novel macrolactin-producing gram-positive bacterial strain MS1721 isolated from the mud at a depth of 30 m in the East China Sea. Methods Taxonomic studies were conducted based on the phenotypic and BIOLOG analysis, 16S rDNA gene sequencing and blast analysis, and fatty acid components analysis. The antibacterial activity of macrolactin S was determined by evaluation models established in our lab. Results MS1721 strain displayed high similarity with genus Bacillus in similarity values between 97%～99%, in which the nearest neighbor was Bacillus amyloliquefaciens with the similarity value of 99.6%. The predominant fatty acid component was 15:1 iso H/13:0 3OH. Conclusions MS1721 strain belongs to the genus Bacillus, and Bacillus amyloliquefaciens MS1721 was proposed as the name for this strain on the basis of the taxonomic data. The second metabolite-macrolactin s produced by this strain exhibited obvious antibacterial activity.