中华眼外伤职业眼病杂志
中華眼外傷職業眼病雜誌
중화안외상직업안병잡지
CHINESE JOURNAL OF OCULAR TRAUMA AND OCCUPATIONAL EYE DISEASE
2012年
6期
401-405
,共5页
小梁切除术%丹参单体IH764-3%纤维增生%青光眼
小樑切除術%丹參單體IH764-3%纖維增生%青光眼
소량절제술%단삼단체IH764-3%섬유증생%청광안
Trabeculectomy%IH764-3%Fibroblast proliferation%Glaucoma
目的 探讨丹参单体(IH764 -3)在兔眼小梁切除术后的抗增生作用.方法 将32只新西兰大耳白兔随机分为4组,每组8只:空白对照组、阳性对照组、实验Ⅰ组、实验Ⅱ组,双眼均行标准小梁切除术,实验Ⅰ组、Ⅱ组分别于术后结膜下注射2g·L-1或5g·L-1丹参单体0.2ml,术后第1周1次/d,第2周1次/2 d,共10次.空白对照组和阳性对照组同样注射等量生理盐水和25 g· L-1的5-氟脲嘧啶(5-FU).术前及术后3d、7d、15 d、30 d各组观察眼压、滤过泡、滤过道开放情况及瘢痕形成的组织结构、增生细胞核抗原阳性细胞计数等.结果 观察术后7d、15 d、30 d功能性滤过泡数、眼压控制情况、增生细胞核抗原(PCNA)阳性细胞等,实验Ⅱ组疗效均优于其他3组.且未发现结膜伤口漏或角膜上皮细胞损伤等副作用.结论 丹参单体在小梁切除术中有抑制成纤维细胞增生的作用.丹参单体应用于小梁切除术中及术后,其抗增生能力较5-FU强,而副作用小,并发症少.
目的 探討丹參單體(IH764 -3)在兔眼小樑切除術後的抗增生作用.方法 將32隻新西蘭大耳白兔隨機分為4組,每組8隻:空白對照組、暘性對照組、實驗Ⅰ組、實驗Ⅱ組,雙眼均行標準小樑切除術,實驗Ⅰ組、Ⅱ組分彆于術後結膜下註射2g·L-1或5g·L-1丹參單體0.2ml,術後第1週1次/d,第2週1次/2 d,共10次.空白對照組和暘性對照組同樣註射等量生理鹽水和25 g· L-1的5-氟脲嘧啶(5-FU).術前及術後3d、7d、15 d、30 d各組觀察眼壓、濾過泡、濾過道開放情況及瘢痕形成的組織結構、增生細胞覈抗原暘性細胞計數等.結果 觀察術後7d、15 d、30 d功能性濾過泡數、眼壓控製情況、增生細胞覈抗原(PCNA)暘性細胞等,實驗Ⅱ組療效均優于其他3組.且未髮現結膜傷口漏或角膜上皮細胞損傷等副作用.結論 丹參單體在小樑切除術中有抑製成纖維細胞增生的作用.丹參單體應用于小樑切除術中及術後,其抗增生能力較5-FU彊,而副作用小,併髮癥少.
목적 탐토단삼단체(IH764 -3)재토안소량절제술후적항증생작용.방법 장32지신서란대이백토수궤분위4조,매조8지:공백대조조、양성대조조、실험Ⅰ조、실험Ⅱ조,쌍안균행표준소량절제술,실험Ⅰ조、Ⅱ조분별우술후결막하주사2g·L-1혹5g·L-1단삼단체0.2ml,술후제1주1차/d,제2주1차/2 d,공10차.공백대조조화양성대조조동양주사등량생리염수화25 g· L-1적5-불뇨밀정(5-FU).술전급술후3d、7d、15 d、30 d각조관찰안압、려과포、려과도개방정황급반흔형성적조직결구、증생세포핵항원양성세포계수등.결과 관찰술후7d、15 d、30 d공능성려과포수、안압공제정황、증생세포핵항원(PCNA)양성세포등,실험Ⅱ조료효균우우기타3조.차미발현결막상구루혹각막상피세포손상등부작용.결론 단삼단체재소량절제술중유억제성섬유세포증생적작용.단삼단체응용우소량절제술중급술후,기항증생능력교5-FU강,이부작용소,병발증소.
Objective To explore the antiproliferative effect of salvia miltiorrhiza monomer IH764-3 on trabeculectomy of rabbit eyes.Methods Thirty-two rabbit eyes were divided into 4 groups randomly with eight rabbits in each group:control group,positive control group,experiment group I and experiment group Ⅱ.Both eyes of each rabbit underwent standard trabeculectomy.Subconjunctival injections of 0.2 mL 2 g/L IH764-3,5 g/L IH764-3,normal saline solution and 25 g/L 5-FU were performed in experiment group Ⅰ,group Ⅱ,control group and positive control group once per day in the first week and once every two days in the second week after surgery.Observation items included preoperative intraocular pressure (IOP),postoperative IOP,filtering bleb,filtering passway,scar structure and proliferating cell nuclear antigen (PCNA).Results Experiment group Ⅱ had better therapeutic effect than other three groups on functional filtering bleb,IOP and PCNA at 7 d,15 d and 30 d postoperatively.Conjunctival wound leaking or corneal epithelial damage had not been found.Conclusion IH764-3 has stronger antiproliferative effect on trabeculectomy than 5-FU with less intraoperative and postoperative side effects.
