CAJ | 학술논문

目的研究贲门腺癌(GCA)中分泌型卷曲相关蛋白1(SFRP1)、分泌型卷曲相关蛋白2(SFRP2)基因启动子区甲基化状态与贲门腺癌的关系.方法采用甲基化特异性PCR(MSP)方法检测GCA癌组织及相应癌旁正常组织中SFRP1和SFRP2基因的甲基化状态,分析其与临床病理特征之间的关系.结果 94例GCA组织中SFRP1、SFRP2基因甲基化率分别为87.2%(82/94)和83.0%(78/94),显著高于癌旁正常组织14.9%(7/47)和55.3%(26/47)(P<0.001).GCA患者中无淋巴结转移组SFRP1基因甲基化发生率73.7%(28/38)显著低于有淋巴结转移组的甲基化率96.4%(54/56)(P<0.05);SFRP2基因的甲基化与有无淋巴结转移无关.SFRP1和SFRP2基因的甲基化与GCA的组织分化无关.63例贲门腺癌组织中SFRP1和SFRP2基因同时发生甲基化,其中有淋巴结转移的36例高于无淋巴结转移的27例,高中分化腺癌组26例,低于低分化腺癌组37例.侵及肌层及浆膜层的16例低于侵及周围软组织的47例,但以上差异均无统计学意义(P>0.05).结论 SFRP1、SFRP2基因可能参与了贲门腺癌的发生发展,并且SFRP1高甲基化状态与贲门腺癌的恶性行为有关.
목적 연구분문선암(GCA)중분비형권곡상관단백1(SFRP1)、분비형권곡상관단백2(SFRP2)기인계동자구갑기화상태여분문선암적관계.방법 채용갑기화특이성PCR(MSP)방법검측GCA암조직급상응암방정상조직중SFRP1화SFRP2기인적갑기화상태,분석기여림상병리특정지간적관계.결과 94례GCA조직중SFRP1、SFRP2기인갑기화솔분별위87.2%(82/94)화83.0%(78/94),현저고우암방정상조직14.9%(7/47)화55.3%(26/47)(P<0.001).GCA환자중무림파결전이조SFRP1기인갑기화발생솔73.7%(28/38)현저저우유림파결전이조적갑기화솔96.4%(54/56)(P<0.05);SFRP2기인적갑기화여유무림파결전이무관.SFRP1화SFRP2기인적갑기화여GCA적조직분화무관.63례분문선암조직중SFRP1화SFRP2기인동시발생갑기화,기중유림파결전이적36례고우무림파결전이적27례,고중분화선암조26례,저우저분화선암조37례.침급기층급장막층적16례저우침급주위연조직적47례,단이상차이균무통계학의의(P>0.05).결론 SFRP1、SFRP2기인가능삼여료분문선암적발생발전,병차SFRP1고갑기화상태여분문선암적악성행위유관.
Objective To investigate the promoter methylation status of SFRP1 and SFRP2 gene in gastric cardia adenocarcinoma (GCA). Methods Methylation specific PCR (MSP) method was used to examine the methylation status of the 5' CpG island of SFRP1 and SFRP2 gene in tumors and corresponding normal tissues. Results Methylation frequencies of SFRP1 and SFRP2 gene in tumor specimens were 87.2 % (82/94) and 83 %(78/94), which was significantly higher than that in corresponding normal tissues (14.9 % and 55.3 %, respectively) (P <0.001). Methylation frequencies of SFRP1 in lymph node metastasis group (96.4 %) was significantly higher than that in no lymph node metastasis group (73.7 %). Methylation frequencies of SFRP1 and SFRP2 gene in poor differentiation group were all higher than that in moderate and poor-moderate differentiation groups, but both of them did not show significant difference(P >0.05). 63 cases of GCA showed both of SFRP1 and SFRP2 gene simultaneous methylation, which including 36 cases of lymph node metastasis group, 27 cases of no lymph node metastasis group. Simultaneous methylation frequencies of SFRP1 and SFRP2 gene in lymph node metastasis group was higher than that in no lymph node metastasis group, poor differentiation group was higher than that in moderate and poor-moderate differentiation groups, but both of them did not show significant difference (P >0.05). Conclusion Promoter methylation of SFRP1 and SFRP2 might be related with oncogenesis of GCA and hypermethylation of SFRP1 gene might be related with the malignant behavior of GCA.