CAJ | 학술논문

巴西固氮螺菌(Azospirillrm brasilence)是重要的植物促生内生菌之一.用gfp基因标记固氮螺菌后接种无菌的水稻和烟草幼苗的根部,限制培养一定时间后,用共聚焦激光显微镜观察,结果表明:除了根部有发荧光的螺菌定殖外,螺菌还分布在茎、叶的表皮细胞,皮层细胞和维管系统组织的细胞间隙.从根、茎、叶器官分离固氮螺菌,都存在有较高的螺菌群体密度.这一结果证明螺菌在植物内存在着从根部向茎、叶顶端的迁移现象.这一发现为研究巴西固氮螺菌在窠主植物体内的迁移运动的机制、与植物细胞间的分子相互作用及其对植物的促生作用奠定了生态学和细胞形态学的基础,也为实际应用提示了进一步的科学依据,具有重要的科学和实践意义.
파서고담라균(Azospirillrm brasilence)시중요적식물촉생내생균지일.용gfp기인표기고담라균후접충무균적수도화연초유묘적근부,한제배양일정시간후,용공취초격광현미경관찰,결과표명:제료근부유발형광적라균정식외,라균환분포재경、협적표피세포,피층세포화유관계통조직적세포간극.종근、경、협기관분리고담라균,도존재유교고적라균군체밀도.저일결과증명라균재식물내존재착종근부향경、협정단적천이현상.저일발현위연구파서고담라균재과주식물체내적천이운동적궤제、여식물세포간적분자상호작용급기대식물적촉생작용전정료생태학화세포형태학적기출,야위실제응용제시료진일보적과학의거,구유중요적과학화실천의의.
Azospirillum brasilense Tarrand, Krieg et Dobereiner is one of the important plant growthpromotion endophytes. A. brasilense Yu62 tagged with gfp gene was inoculated into roots of rice and tobacco seedlings, which were then, cultured in gnotobiotic condition. At a certain days after inoculation the different portions of the seedling were observed under laser confocal microscope, resulting in that A.brasilense Yu62 bacteria were colonized in epidermal and cortical cells, intercellular spaces and vascular system of stem and leaf tissue interiors besides in roots. Higher populations of the bacteria isolated from roots, stems and leaves indicated that A. brasilense Yu62 bacteria could ascend themselves from roots to stems and leaves of rice and tobacco. This observation lays down the foundation for ecology and cell morphology of bacterial migration inside plants, interaction between A. brasilense Yu62 bacteria and host cells as well as the plant-growth promotion, provides scientific basis for further application, and is of importance in science and practice.