CAJ | 학술논문

为了研究不同水分条件下组成型根系性状和适应性根系性状的遗传机制,利用由IR64/Azucena发展的双单倍体(DH)群体分析了淹水和干旱条件下水稻幼苗种子根长(SRL)、不定根数(ARN)、总根干重(RW)及其对应的相对参数(干旱和淹水条件下根系性状的比值)的QTLs.淹水与干旱条件下检测到一个共同的种子根长QTL和一个共同的总根干重QTL.同时对前人发表的遗传群体定位的根系性状QrLs进行比较分析,检测到几个共同的根系性状QTLs.对与细胞伸长、分裂相关的候选基因进行了定位,其中4个细胞壁相关的ESTs(OsEXP2,OsEXP4,EXT和Xet)被定位在与不同水分条件下检测出的根系性状QTLs的相同区间.
위료연구불동수분조건하조성형근계성상화괄응성근계성상적유전궤제,이용유IR64/Azucena발전적쌍단배체(DH)군체분석료엄수화간한조건하수도유묘충자근장(SRL)、불정근수(ARN)、총근간중(RW)급기대응적상대삼수(간한화엄수조건하근계성상적비치)적QTLs.엄수여간한조건하검측도일개공동적충자근장QTL화일개공동적총근간중QTL.동시대전인발표적유전군체정위적근계성상QrLs진행비교분석,검측도궤개공동적근계성상QTLs.대여세포신장、분렬상관적후선기인진행료정위,기중4개세포벽상관적ESTs(OsEXP2,OsEXP4,EXT화Xet)피정위재여불동수분조건하검측출적근계성상QTLs적상동구간.
To investigate the genetic factors underlying constitutive and adaptive root growth under different water-supply conditions, a double haploid (DH) population, derived from a cross between lowland rice variety IR64 and upland rice variety Azucena,with 284 molecular markers was used in cylindrical pot experiments. Several QTLs for seminal root length (SRL), adventitious root number (ARN) and total root dry weight (RW) respectively, under both flooding and upland conditions were detected. Two identical QTLs for SRL and RW were found under flooding and upland conditions. The relative parameters defined as the ratio of parameters under the two water-supply conditions were also used for QTL analysis. A comparative analysis among different genetic populations was performed for the QTLs for root traits and several consistent QTLs for root traits across genetic backgrounds were detected. Candidate genes for cell expansion and elongation were used for comparative mapping with the detected QTLs. Four cell wall-related expressed sequence tags (ESTs) for OsEXP2, OsEXP4, EXT and Xet were mapped on the intervals carrying the QTLs for root traits.