中国环境科学
中國環境科學
중국배경과학
CHINA ENVIRONMENTAL SCIENCE
2010年
1期
76-81
,共6页
理化因素%脱氮硫杆菌%自养反硝化%硫代硫酸钠
理化因素%脫氮硫桿菌%自養反硝化%硫代硫痠鈉
이화인소%탈담류간균%자양반초화%류대류산납
physicochemical factor%Thiobacillus denitrificans%autotrophic denitrification%sodium thiosulphate
从连续运行的UASB反应器厌氧污泥中分离得到一株脱氮硫杆菌T.d.a,采用分批摇床试验,采用脱氮硫杆菌标准培养基,以硫代硫酸钠为硫源,研究pH值、温度、氮源(NO_3-N,NH_4~+-N)、能源(S_2O_3~(2-))、碳源(HCO_3)、葡萄糖、无机盐(P,Mg~(2-),Fe~(2-))对该菌株自养反硝化的影响.结果表明,在pH6.5～8.0,温度20-35℃的范围内,T.d.a对NO_3-N均有较高的去除速率,其最佳反硝化pH值为7.04,温度为27.40℃.T.d.a对<416mg/L的NO_3-N有较好的适应性,>554mg/L的NO_3-N对T.d.a有一定的抑制作用;T.d.a反硝化所需NH_4~+-N的限制浓度为2.62mg/L;S_2O_3~(2-)浓度对T.d.a反硝化的影响主要取决于其与NO_3的比例关系,在NO_3过量的情况下,NO_3-N去除率与加入的S_2O_3~(2-)量成近似的正比关系.T.d.a以HCO_3作为无机碳源时其限制浓度为29.05mg/L;0～2000mg/L的葡萄糖对NO_3-N去除率没有明显影响.P和Mg~(2-)的限制浓度分别为0.034,0.059mg/L,Fe~(2+)的限制浓度低于0.058mg/L.
從連續運行的UASB反應器厭氧汙泥中分離得到一株脫氮硫桿菌T.d.a,採用分批搖床試驗,採用脫氮硫桿菌標準培養基,以硫代硫痠鈉為硫源,研究pH值、溫度、氮源(NO_3-N,NH_4~+-N)、能源(S_2O_3~(2-))、碳源(HCO_3)、葡萄糖、無機鹽(P,Mg~(2-),Fe~(2-))對該菌株自養反硝化的影響.結果錶明,在pH6.5～8.0,溫度20-35℃的範圍內,T.d.a對NO_3-N均有較高的去除速率,其最佳反硝化pH值為7.04,溫度為27.40℃.T.d.a對<416mg/L的NO_3-N有較好的適應性,>554mg/L的NO_3-N對T.d.a有一定的抑製作用;T.d.a反硝化所需NH_4~+-N的限製濃度為2.62mg/L;S_2O_3~(2-)濃度對T.d.a反硝化的影響主要取決于其與NO_3的比例關繫,在NO_3過量的情況下,NO_3-N去除率與加入的S_2O_3~(2-)量成近似的正比關繫.T.d.a以HCO_3作為無機碳源時其限製濃度為29.05mg/L;0～2000mg/L的葡萄糖對NO_3-N去除率沒有明顯影響.P和Mg~(2-)的限製濃度分彆為0.034,0.059mg/L,Fe~(2+)的限製濃度低于0.058mg/L.
종련속운행적UASB반응기염양오니중분리득도일주탈담류간균T.d.a,채용분비요상시험,채용탈담류간균표준배양기,이류대류산납위류원,연구pH치、온도、담원(NO_3-N,NH_4~+-N)、능원(S_2O_3~(2-))、탄원(HCO_3)、포도당、무궤염(P,Mg~(2-),Fe~(2-))대해균주자양반초화적영향.결과표명,재pH6.5～8.0,온도20-35℃적범위내,T.d.a대NO_3-N균유교고적거제속솔,기최가반초화pH치위7.04,온도위27.40℃.T.d.a대<416mg/L적NO_3-N유교호적괄응성,>554mg/L적NO_3-N대T.d.a유일정적억제작용;T.d.a반초화소수NH_4~+-N적한제농도위2.62mg/L;S_2O_3~(2-)농도대T.d.a반초화적영향주요취결우기여NO_3적비례관계,재NO_3과량적정황하,NO_3-N거제솔여가입적S_2O_3~(2-)량성근사적정비관계.T.d.a이HCO_3작위무궤탄원시기한제농도위29.05mg/L;0～2000mg/L적포도당대NO_3-N거제솔몰유명현영향.P화Mg~(2-)적한제농도분별위0.034,0.059mg/L,Fe~(2+)적한제농도저우0.058mg/L.
A strain of Thiobacillus denitrificans (T.d.a) was isolated from anaerobic sludge of consecutive running UASB reactor. Based on culture media of Thiobacillus denitrificans, the effects of pH, temperature, NO_3 -N, NH_4~+-N,S_2O_3~(2-), HCO_3, C_6H_(12)O_6, P, Mg~(2+) and Fe~(2+) on the denitrification by T.d.a were studied in batch experiments using sodium thiosulphate as sulfur source. The nitrate removal velocities were high at pH 6.5～8.0 and temperature 20～35℃. The optimal conditions were pH 7.04 and 27.40℃. T.d.a exhibited a good adaptation when the concentration of NO_3-N was below 416mg/L. At 554mg/L or above, however, the nitrate removal was markedly inhibited. The denitrification needed NH_4~+-N and its limiting concentration was 2.62mg/L. The effect of the concentration of S_2O_3~(2-) on the denitrificaion depended on different sulfur to nitrogen ratios. When NO_3 was enough, the NO_3-N removal efficiency was in similar direct proportion to the S_2O_3~(2-) added[0]. Using HCO_3 as sole carbon source, its limiting concentration was 29.05mg/L. The presence of glucose up to 2000mg/L didn't significantly affect the nitrate removal efficiency. The limiting concentrations of P and Mg~(2+) were 0.034mg/L and 0.059mg/L respectively. The limiting concentration of Fe~(2+) was below 0.058mg/L.
